Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia
Abstract Background Tibial dyschondroplasia (TD) is a bone disorder in which dead chondrocytes accumulate as a result of apoptosis and non-vascularization in the tibial bone of broiler chickens. The pathogenicity of TD is under extensive research but is yet not fully understood. Several studies have...
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| Format: | Article |
| Language: | English |
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BMC
2022-04-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-022-08494-9 |
| _version_ | 1818127743070502912 |
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| author | Ali Raza Jahejo Sayyad Ali Raza Bukhari Nasir Rajput Nazeer Hussain Kalhoro Imdad Hussain Leghari Sayed Haidar Abbas Raza Zhen Li Wen-zhong Liu Wen-xia Tian |
| author_facet | Ali Raza Jahejo Sayyad Ali Raza Bukhari Nasir Rajput Nazeer Hussain Kalhoro Imdad Hussain Leghari Sayed Haidar Abbas Raza Zhen Li Wen-zhong Liu Wen-xia Tian |
| author_sort | Ali Raza Jahejo |
| collection | DOAJ |
| description | Abstract Background Tibial dyschondroplasia (TD) is a bone disorder in which dead chondrocytes accumulate as a result of apoptosis and non-vascularization in the tibial bone of broiler chickens. The pathogenicity of TD is under extensive research but is yet not fully understood. Several studies have linked it to apoptosis and non-vascularization in the tibial growth plate (GP). We conceived the idea to find the differentially expressed genes (DEGs) in chicken erythrocytes which vary in expression over time using a likelihood-ratio test (LRT). Thiram was used to induce TD in chickens, and then injected Ex-FABP protein at 0, 20, and 50 μg.kg-1 to evaluate its therapeutic effect on 30 screened immunity and angiogenesis-related genes using quantitative PCR (qPCR). The histopathology was also performed in TD chickens to explore the shape, circularity, arrangements of chondrocytes and blood vessels. Results Clinical lameness was observed in TD chickens, which decreased with the injection of Ex-FABP. Histopathological findings support Ex-FABP as a therapeutic agent for the morphology and vascularization of affected chondrocytes in TD chickens. qPCR results of 10 immunity (TLR2, TLR3, TLR4, TLR5, TLR7, TLR15, IL-7, MyD88, MHCII, and TRAF6) and 20 angiogenesis-related genes (ITGAV, ITGA2, ITGB2, ITGB3, ITGA5, IL1R1, TBXA2R, RPL17, F13A1, CLU, RAC2, RAP1B, GIT1, FYN, IQGAP2, PTCH1, NCOR2, VAV-like, PTPN11, MAML3) regulated when Ex-FABP is injected to TD chickens. Conclusion Immunity and angiogenesis-related genes can be responsible for apoptosis of chondrocytes and vascularization in tibial GP. Injection of Ex-FABP protein to thiram induced TD chickens decrease the chondrocytes damage and improves vascularization. |
| first_indexed | 2024-12-11T07:22:12Z |
| format | Article |
| id | doaj.art-67c3dcb1b98e413c965fa640a2e1cf52 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T07:22:12Z |
| publishDate | 2022-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-67c3dcb1b98e413c965fa640a2e1cf522022-12-22T01:16:04ZengBMCBMC Genomics1471-21642022-04-0123111710.1186/s12864-022-08494-9Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasiaAli Raza Jahejo0Sayyad Ali Raza Bukhari1Nasir Rajput2Nazeer Hussain Kalhoro3Imdad Hussain Leghari4Sayed Haidar Abbas Raza5Zhen Li6Wen-zhong Liu7Wen-xia Tian8College of Animal Science, Shanxi Agricultural UniversityDepartment of Biotechnology, University of SargodhaDepartment of Poultry Husbandry, Sindh Agriculture UniversitySindh Institute of Animal HealthDepartment of Poultry Husbandry, Sindh Agriculture UniversityCollege of Animal Science and Technology, Northwest A&F UniversityCollege of Veterinary Medicine, Shanxi Agricultural UniversityCollege of Animal Science, Shanxi Agricultural UniversityCollege of Veterinary Medicine, Shanxi Agricultural UniversityAbstract Background Tibial dyschondroplasia (TD) is a bone disorder in which dead chondrocytes accumulate as a result of apoptosis and non-vascularization in the tibial bone of broiler chickens. The pathogenicity of TD is under extensive research but is yet not fully understood. Several studies have linked it to apoptosis and non-vascularization in the tibial growth plate (GP). We conceived the idea to find the differentially expressed genes (DEGs) in chicken erythrocytes which vary in expression over time using a likelihood-ratio test (LRT). Thiram was used to induce TD in chickens, and then injected Ex-FABP protein at 0, 20, and 50 μg.kg-1 to evaluate its therapeutic effect on 30 screened immunity and angiogenesis-related genes using quantitative PCR (qPCR). The histopathology was also performed in TD chickens to explore the shape, circularity, arrangements of chondrocytes and blood vessels. Results Clinical lameness was observed in TD chickens, which decreased with the injection of Ex-FABP. Histopathological findings support Ex-FABP as a therapeutic agent for the morphology and vascularization of affected chondrocytes in TD chickens. qPCR results of 10 immunity (TLR2, TLR3, TLR4, TLR5, TLR7, TLR15, IL-7, MyD88, MHCII, and TRAF6) and 20 angiogenesis-related genes (ITGAV, ITGA2, ITGB2, ITGB3, ITGA5, IL1R1, TBXA2R, RPL17, F13A1, CLU, RAC2, RAP1B, GIT1, FYN, IQGAP2, PTCH1, NCOR2, VAV-like, PTPN11, MAML3) regulated when Ex-FABP is injected to TD chickens. Conclusion Immunity and angiogenesis-related genes can be responsible for apoptosis of chondrocytes and vascularization in tibial GP. Injection of Ex-FABP protein to thiram induced TD chickens decrease the chondrocytes damage and improves vascularization.https://doi.org/10.1186/s12864-022-08494-9AngiogenesisBone-deformityBiomarker-genesBroilerEx-FABPImmunity |
| spellingShingle | Ali Raza Jahejo Sayyad Ali Raza Bukhari Nasir Rajput Nazeer Hussain Kalhoro Imdad Hussain Leghari Sayed Haidar Abbas Raza Zhen Li Wen-zhong Liu Wen-xia Tian Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia BMC Genomics Angiogenesis Bone-deformity Biomarker-genes Broiler Ex-FABP Immunity |
| title | Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia |
| title_full | Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia |
| title_fullStr | Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia |
| title_full_unstemmed | Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia |
| title_short | Transcriptome-based biomarker gene screening and evaluation of the extracellular fatty acid-binding protein (Ex-FABP) on immune and angiogenesis-related genes in chicken erythrocytes of tibial dyschondroplasia |
| title_sort | transcriptome based biomarker gene screening and evaluation of the extracellular fatty acid binding protein ex fabp on immune and angiogenesis related genes in chicken erythrocytes of tibial dyschondroplasia |
| topic | Angiogenesis Bone-deformity Biomarker-genes Broiler Ex-FABP Immunity |
| url | https://doi.org/10.1186/s12864-022-08494-9 |
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