Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids
Rheumatoid arthritis (RA) disease activity fluctuates over time. The disease activity score 28 (DAS28<sub>ESR</sub>) is a widely used and validated scoring system for assessing RA activity; however, it requires time and expertise. This study aimed to develop a new molecular assay capable...
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MDPI AG
2020-10-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/11/3499 |
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| author | Jung Hee Koh Saseong Lee Hyun-Sook Kim Kyuheon Lee Chang Seop Lee Seung-Ah Yoo Naeun Lee Wan-Uk Kim |
| author_facet | Jung Hee Koh Saseong Lee Hyun-Sook Kim Kyuheon Lee Chang Seop Lee Seung-Ah Yoo Naeun Lee Wan-Uk Kim |
| author_sort | Jung Hee Koh |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) disease activity fluctuates over time. The disease activity score 28 (DAS28<sub>ESR</sub>) is a widely used and validated scoring system for assessing RA activity; however, it requires time and expertise. This study aimed to develop a new molecular assay capable of rapidly and objectively assessing RA activity. We used a rapid immuno-assay system (FREND™) to measure soluble CD14 (sCD14) levels, which reflect the DAS28<sub>ESR</sub>. SCD14 concentrations in urine and serum of RA patients were measured, and RA activity and responses to anti-rheumatic drugs were examined at baseline and after 6 months. FREND™ quantified sCD14 levels in a drop of serum and urine accurately and within 5 min. Serum sCD14 concentrations and its changes correlated well with disease activity and treatment responses, and the results were comparable to C-reactive protein. The new composite indices, including the DAS28<sub>CD14</sub> and simplified DAS<sub>CD14</sub>, better detected RA activity than a single sCD14 value and correlated strongly with the DAS28<sub>ESR</sub>. These indices exhibited excellent diagnostic performance for discriminating a good response 6 months after treatment. We developed a new system for assessing RA activity and therapeutic outcome within 5 min. CD14-based composite indices may have utility for accurate and frequent monitoring of RA status. |
| first_indexed | 2024-03-10T15:14:50Z |
| format | Article |
| id | doaj.art-67c51a1f298a4ef6a4cc46ddb065797f |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T15:14:50Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-67c51a1f298a4ef6a4cc46ddb065797f2023-11-20T19:03:01ZengMDPI AGJournal of Clinical Medicine2077-03832020-10-01911349910.3390/jcm9113499Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-FluidsJung Hee Koh0Saseong Lee1Hyun-Sook Kim2Kyuheon Lee3Chang Seop Lee4Seung-Ah Yoo5Naeun Lee6Wan-Uk Kim7Division of Rheumatology, Department of Internal Medicine, The Catholic University of Korea, Seoul 06591, KoreaCenter for Integrative Rheumatoid Transcriptomics and Dynamics, The Catholic University of Korea, Seoul 06591, KoreaDepartment of Internal Medicine, Soonchunhyang University College of Medicine, Seoul 04401, KoreaNanoEntek Inc., Seoul 08389, KoreaNanoEntek Inc., Seoul 08389, KoreaCenter for Integrative Rheumatoid Transcriptomics and Dynamics, The Catholic University of Korea, Seoul 06591, KoreaCenter for Integrative Rheumatoid Transcriptomics and Dynamics, The Catholic University of Korea, Seoul 06591, KoreaDivision of Rheumatology, Department of Internal Medicine, The Catholic University of Korea, Seoul 06591, KoreaRheumatoid arthritis (RA) disease activity fluctuates over time. The disease activity score 28 (DAS28<sub>ESR</sub>) is a widely used and validated scoring system for assessing RA activity; however, it requires time and expertise. This study aimed to develop a new molecular assay capable of rapidly and objectively assessing RA activity. We used a rapid immuno-assay system (FREND™) to measure soluble CD14 (sCD14) levels, which reflect the DAS28<sub>ESR</sub>. SCD14 concentrations in urine and serum of RA patients were measured, and RA activity and responses to anti-rheumatic drugs were examined at baseline and after 6 months. FREND™ quantified sCD14 levels in a drop of serum and urine accurately and within 5 min. Serum sCD14 concentrations and its changes correlated well with disease activity and treatment responses, and the results were comparable to C-reactive protein. The new composite indices, including the DAS28<sub>CD14</sub> and simplified DAS<sub>CD14</sub>, better detected RA activity than a single sCD14 value and correlated strongly with the DAS28<sub>ESR</sub>. These indices exhibited excellent diagnostic performance for discriminating a good response 6 months after treatment. We developed a new system for assessing RA activity and therapeutic outcome within 5 min. CD14-based composite indices may have utility for accurate and frequent monitoring of RA status.https://www.mdpi.com/2077-0383/9/11/3499rapid quantitative immunoassaysFREND™ systemsoluble CD14rheumatoid arthritisbiomarker |
| spellingShingle | Jung Hee Koh Saseong Lee Hyun-Sook Kim Kyuheon Lee Chang Seop Lee Seung-Ah Yoo Naeun Lee Wan-Uk Kim Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids Journal of Clinical Medicine rapid quantitative immunoassays FREND™ system soluble CD14 rheumatoid arthritis biomarker |
| title | Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids |
| title_full | Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids |
| title_fullStr | Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids |
| title_full_unstemmed | Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids |
| title_short | Development of Monitoring System for Assessing Rheumatoid Arthritis within 5 Minutes Using a Drop of Bio-Fluids |
| title_sort | development of monitoring system for assessing rheumatoid arthritis within 5 minutes using a drop of bio fluids |
| topic | rapid quantitative immunoassays FREND™ system soluble CD14 rheumatoid arthritis biomarker |
| url | https://www.mdpi.com/2077-0383/9/11/3499 |
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