Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection
Objective We aimed to investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi) in vitro and in vivo. Materials and Methods Phosphorylcholine (PC) expression of 21 strai...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2020.1808425 |
| _version_ | 1818915229381165056 |
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| author | Hiroyuki Iuchi Junichiro Ohori Takayuki Kyutoku Kotoko Ito Masaki Kawabata |
| author_facet | Hiroyuki Iuchi Junichiro Ohori Takayuki Kyutoku Kotoko Ito Masaki Kawabata |
| author_sort | Hiroyuki Iuchi |
| collection | DOAJ |
| description | Objective We aimed to investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi) in vitro and in vivo. Materials and Methods Phosphorylcholine (PC) expression of 21 strains each of Spn and NTHi was evaluated using fluorescence-activated cell sorting; the adherence of bacteria to Detroit 562 cells and to the nasal mucosa of BALB/c mice was determined. MPC polymer-mediated inhibitory effects were compared with PC-keyhole limpet hemocyanin (PC-KLH)-mediated inhibitory effects. Results In vitro experiments showed that pretreatment with MPC polymer markedly inhibited the adherence of Spn and NTHi in a concentration dose–dependent manner independently of PC expression. No correlation was observed between PC expression and MPC polymer-mediated inhibitory effects. Contrarily, there was a significant negative correlation between PC-KLH-mediated inhibitory effects and PC expression in Spn and NTHi. The same results were obtained via in vivo experiments. The MPC polymer did not affect the histology of the nasal mucosa. Conclusions MPC polymer might be effective to reduce the occurrence of upper respiratory tract infection caused by Spn and NTHi and could be applied for the development of local treatments, such as topical gargles and nebulizer medications. |
| first_indexed | 2024-12-19T23:58:57Z |
| format | Article |
| id | doaj.art-67c7140e04cd48a493b4954e6df12161 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-19T23:58:57Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-67c7140e04cd48a493b4954e6df121612022-12-21T20:00:55ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972020-01-0112110.1080/20002297.2020.18084251808425Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infectionHiroyuki Iuchi0Junichiro Ohori1Takayuki Kyutoku2Kotoko Ito3Masaki Kawabata4Kagoshima University Graduate School of Medical and Dental SciencesKagoshima University Graduate School of Medical and Dental SciencesKagoshima University Graduate School of Medical and Dental SciencesKagoshima University Graduate School of Medical and Dental SciencesKagoshima University Graduate School of Medical and Dental SciencesObjective We aimed to investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi) in vitro and in vivo. Materials and Methods Phosphorylcholine (PC) expression of 21 strains each of Spn and NTHi was evaluated using fluorescence-activated cell sorting; the adherence of bacteria to Detroit 562 cells and to the nasal mucosa of BALB/c mice was determined. MPC polymer-mediated inhibitory effects were compared with PC-keyhole limpet hemocyanin (PC-KLH)-mediated inhibitory effects. Results In vitro experiments showed that pretreatment with MPC polymer markedly inhibited the adherence of Spn and NTHi in a concentration dose–dependent manner independently of PC expression. No correlation was observed between PC expression and MPC polymer-mediated inhibitory effects. Contrarily, there was a significant negative correlation between PC-KLH-mediated inhibitory effects and PC expression in Spn and NTHi. The same results were obtained via in vivo experiments. The MPC polymer did not affect the histology of the nasal mucosa. Conclusions MPC polymer might be effective to reduce the occurrence of upper respiratory tract infection caused by Spn and NTHi and could be applied for the development of local treatments, such as topical gargles and nebulizer medications.http://dx.doi.org/10.1080/20002297.2020.18084252-methacryloyloxyethyl phosphorylcholinenontypeable haemophilus influenzastreptococcuspneumoniaphosphorylcholineinhibitory effectdetroit 562 cellsbalb/c mice |
| spellingShingle | Hiroyuki Iuchi Junichiro Ohori Takayuki Kyutoku Kotoko Ito Masaki Kawabata Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection Journal of Oral Microbiology 2-methacryloyloxyethyl phosphorylcholine nontypeable haemophilus influenza streptococcuspneumonia phosphorylcholine inhibitory effect detroit 562 cells balb/c mice |
| title | Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection |
| title_full | Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection |
| title_fullStr | Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection |
| title_full_unstemmed | Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection |
| title_short | Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection |
| title_sort | inhibitory effects of 2 methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper respiratory tract infection |
| topic | 2-methacryloyloxyethyl phosphorylcholine nontypeable haemophilus influenza streptococcuspneumonia phosphorylcholine inhibitory effect detroit 562 cells balb/c mice |
| url | http://dx.doi.org/10.1080/20002297.2020.1808425 |
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