Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients
<p>Abstract</p> <p>Background</p> <p>The immune system reconstitution in HIV-1- infected patients undergoing combined antiretroviral therapy is routinely evaluated by T-cell phenotyping, even though the infection also impairs the B-cell mediated immunity. To find new la...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-05-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://www.translational-medicine.com/content/10/1/94 |
| _version_ | 1828258912107757568 |
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| author | Quiros-Roldan Eugenia Serana Federico Chiarini Marco Zanotti Cinzia Sottini Alessandra Gotti Daria Torti Carlo Caimi Luigi Imberti Luisa |
| author_facet | Quiros-Roldan Eugenia Serana Federico Chiarini Marco Zanotti Cinzia Sottini Alessandra Gotti Daria Torti Carlo Caimi Luigi Imberti Luisa |
| author_sort | Quiros-Roldan Eugenia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The immune system reconstitution in HIV-1- infected patients undergoing combined antiretroviral therapy is routinely evaluated by T-cell phenotyping, even though the infection also impairs the B-cell mediated immunity. To find new laboratory markers of therapy effectiveness, both B- and T- immune recovery were evaluated by means of a follow-up study of long-term treated HIV-1- infected patients, with a special focus on the measure of new B- and T-lymphocyte production.</p> <p>Methods</p> <p>A longitudinal analysis was performed in samples obtained from HIV-1-infected patients before therapy beginning and after 6, 12, and 72 months with a duplex real-time PCR allowing the detection of K-deleting recombination excision circles (KRECs) and T-cell receptor excision circles (TRECs), as measures of bone-marrow and thymic output, respectively. A cross sectional analysis was performed to detect B- and T-cell subsets by flow cytometry in samples obtained at the end of the follow-up, which were compared to those of untreated HIV-1-infected patients and uninfected controls.</p> <p>Results</p> <p>The kinetics and the timings of B- and T-cell release from the bone marrow and thymus during antiretroviral therapy were substantially different, with a decreased B-cell release and an increased thymic output after the prolonged therapy. The multivariable regression analysis showed that a longer pre-therapy infection duration predicts a minor TREC increase and a major KREC reduction.</p> <p>Conclusions</p> <p>The quantification of KRECs and TRECs represents an improved method to monitor the effects of therapies capable of influencing the immune cell pool composition in HIV-1-infected patients.</p> |
| first_indexed | 2024-04-13T02:59:46Z |
| format | Article |
| id | doaj.art-67cb46619217449088809d4116ae51f0 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-13T02:59:46Z |
| publishDate | 2012-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-67cb46619217449088809d4116ae51f02022-12-22T03:05:28ZengBMCJournal of Translational Medicine1479-58762012-05-011019410.1186/1479-5876-10-94Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patientsQuiros-Roldan EugeniaSerana FedericoChiarini MarcoZanotti CinziaSottini AlessandraGotti DariaTorti CarloCaimi LuigiImberti Luisa<p>Abstract</p> <p>Background</p> <p>The immune system reconstitution in HIV-1- infected patients undergoing combined antiretroviral therapy is routinely evaluated by T-cell phenotyping, even though the infection also impairs the B-cell mediated immunity. To find new laboratory markers of therapy effectiveness, both B- and T- immune recovery were evaluated by means of a follow-up study of long-term treated HIV-1- infected patients, with a special focus on the measure of new B- and T-lymphocyte production.</p> <p>Methods</p> <p>A longitudinal analysis was performed in samples obtained from HIV-1-infected patients before therapy beginning and after 6, 12, and 72 months with a duplex real-time PCR allowing the detection of K-deleting recombination excision circles (KRECs) and T-cell receptor excision circles (TRECs), as measures of bone-marrow and thymic output, respectively. A cross sectional analysis was performed to detect B- and T-cell subsets by flow cytometry in samples obtained at the end of the follow-up, which were compared to those of untreated HIV-1-infected patients and uninfected controls.</p> <p>Results</p> <p>The kinetics and the timings of B- and T-cell release from the bone marrow and thymus during antiretroviral therapy were substantially different, with a decreased B-cell release and an increased thymic output after the prolonged therapy. The multivariable regression analysis showed that a longer pre-therapy infection duration predicts a minor TREC increase and a major KREC reduction.</p> <p>Conclusions</p> <p>The quantification of KRECs and TRECs represents an improved method to monitor the effects of therapies capable of influencing the immune cell pool composition in HIV-1-infected patients.</p>http://www.translational-medicine.com/content/10/1/94KRECsTRECsHIV-1cARTT lymphocytesB lymphocytes |
| spellingShingle | Quiros-Roldan Eugenia Serana Federico Chiarini Marco Zanotti Cinzia Sottini Alessandra Gotti Daria Torti Carlo Caimi Luigi Imberti Luisa Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients Journal of Translational Medicine KRECs TRECs HIV-1 cART T lymphocytes B lymphocytes |
| title | Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients |
| title_full | Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients |
| title_fullStr | Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients |
| title_full_unstemmed | Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients |
| title_short | Effects of combined antiretroviral therapy on B- and T-cell release from production sites in long-term treated HIV-1<sup>+</sup> patients |
| title_sort | effects of combined antiretroviral therapy on b and t cell release from production sites in long term treated hiv 1 sup sup patients |
| topic | KRECs TRECs HIV-1 cART T lymphocytes B lymphocytes |
| url | http://www.translational-medicine.com/content/10/1/94 |
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