Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast
Nanoemulsions (NEs) have been made for improving the delivery and disperse of bioactive compounds. In this study, it was found that the best ingredients for the stable Massoia lactone-loaded and food-grade NEs making were 560.0 µL of Tween-80, 240.0 µL of Span-80 and 200.0 µL of Massoia lactone. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/10/3/339 |
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| author | Li Yuan Hong-Qian Zhang Zhe Chi Guang-Lei Liu Zhen-Ming Chi |
| author_facet | Li Yuan Hong-Qian Zhang Zhe Chi Guang-Lei Liu Zhen-Ming Chi |
| author_sort | Li Yuan |
| collection | DOAJ |
| description | Nanoemulsions (NEs) have been made for improving the delivery and disperse of bioactive compounds. In this study, it was found that the best ingredients for the stable Massoia lactone-loaded and food-grade NEs making were 560.0 µL of Tween-80, 240.0 µL of Span-80 and 200.0 µL of Massoia lactone. Then, 9.0 mL of distilled water was titrated into the mixture under continuous magnetic stirring (750 rotations min<sup>−1</sup>) with about 2 drops per second for 20 min. Finally, the system was treated by ultrasonication using an ultrasonic generator (180 W and 22 KHz) for 5 min. All the prepared particles with a mean droplet diameter of 43 nm were spherical, had uniform size distribution and were equally distributed in the Massoia lactone-loaded NEs. The obtained Massoia lactone-loaded nanoemulsions (NEs) were very stable without changes of the mean droplet diameter and polydispersity indexes (PDI) for over two months under different conditions. As with free Massoia lactone, Massoia lactone loaded in the NEs had high anti-fungal activity against <i>Metschnikowia bicuspidate</i> LIAO, a pathogenic yeast causing milky disease in the Chinese mitten crab by damaging its cell membrane and causing cellular necrosis. Massoia lactone loaded in the NEs also had the DPPH radical scavenging activity and the hydroxyl radical scavenging activity. |
| first_indexed | 2024-03-09T19:35:35Z |
| format | Article |
| id | doaj.art-67d1312d2a064c5e95125b9acc715a6d |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-09T19:35:35Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-67d1312d2a064c5e95125b9acc715a6d2023-11-24T01:56:56ZengMDPI AGJournal of Marine Science and Engineering2077-13122022-03-0110333910.3390/jmse10030339Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic YeastLi Yuan0Hong-Qian Zhang1Zhe Chi2Guang-Lei Liu3Zhen-Ming Chi4College of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, ChinaCollege of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, ChinaCollege of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, ChinaCollege of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, ChinaCollege of Marine Life Science, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, ChinaNanoemulsions (NEs) have been made for improving the delivery and disperse of bioactive compounds. In this study, it was found that the best ingredients for the stable Massoia lactone-loaded and food-grade NEs making were 560.0 µL of Tween-80, 240.0 µL of Span-80 and 200.0 µL of Massoia lactone. Then, 9.0 mL of distilled water was titrated into the mixture under continuous magnetic stirring (750 rotations min<sup>−1</sup>) with about 2 drops per second for 20 min. Finally, the system was treated by ultrasonication using an ultrasonic generator (180 W and 22 KHz) for 5 min. All the prepared particles with a mean droplet diameter of 43 nm were spherical, had uniform size distribution and were equally distributed in the Massoia lactone-loaded NEs. The obtained Massoia lactone-loaded nanoemulsions (NEs) were very stable without changes of the mean droplet diameter and polydispersity indexes (PDI) for over two months under different conditions. As with free Massoia lactone, Massoia lactone loaded in the NEs had high anti-fungal activity against <i>Metschnikowia bicuspidate</i> LIAO, a pathogenic yeast causing milky disease in the Chinese mitten crab by damaging its cell membrane and causing cellular necrosis. Massoia lactone loaded in the NEs also had the DPPH radical scavenging activity and the hydroxyl radical scavenging activity.https://www.mdpi.com/2077-1312/10/3/339liamocinmarine derived <i>Aureobasidium</i>massoia lactone loaded NEsanti-fungal activityanti-oxidant activity |
| spellingShingle | Li Yuan Hong-Qian Zhang Zhe Chi Guang-Lei Liu Zhen-Ming Chi Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast Journal of Marine Science and Engineering liamocin marine derived <i>Aureobasidium</i> massoia lactone loaded NEs anti-fungal activity anti-oxidant activity |
| title | Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast |
| title_full | Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast |
| title_fullStr | Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast |
| title_full_unstemmed | Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast |
| title_short | Making of Massoia Lactone-Loaded and Food-Grade Nanoemulsions and Their Bioactivities against a Pathogenic Yeast |
| title_sort | making of massoia lactone loaded and food grade nanoemulsions and their bioactivities against a pathogenic yeast |
| topic | liamocin marine derived <i>Aureobasidium</i> massoia lactone loaded NEs anti-fungal activity anti-oxidant activity |
| url | https://www.mdpi.com/2077-1312/10/3/339 |
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