<i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites
Polycyclic aromatic hydrocarbons (PAHs) are organic pollutants widely present in industrial sites. Microbial degradation is an effective method of removing PAHs. The identification of microorganisms that have important ecological functions at the site is of great significance for PAH removal. We col...
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/6/1109 |
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| author | Meng Zhou Zishu Liu Jiaqi Wang Yuxiang Zhao Baolan Hu |
| author_facet | Meng Zhou Zishu Liu Jiaqi Wang Yuxiang Zhao Baolan Hu |
| author_sort | Meng Zhou |
| collection | DOAJ |
| description | Polycyclic aromatic hydrocarbons (PAHs) are organic pollutants widely present in industrial sites. Microbial degradation is an effective method of removing PAHs. The identification of microorganisms that have important ecological functions at the site is of great significance for PAH removal. We collected soil samples at three depths in the range of 0–100 cm at 70-day intervals at the coking site and explored the degradation of PAHs. We combined molecular ecology networking, metagenomics, and genome assembly to search for microorganisms that persist, dominate, and affect the microbial community construction in the degradation process and analyzed their adaptation strategies. The results showed that 15.78 mg/kg of PAHs naturally decayed, and 13.33 mg/kg of PAHs migrated from 30–100 cm to 0–30 cm in the soil. <i>Sphingomonas</i>, which occupied a niche advantage, was both the core and keystone microorganism, and its spatial distribution pattern and temporal change dynamics were consistent with those of PAHs. We assembled the genome of <i>Sphingomonas</i> sp., revealing its multiple potential for degrading PAHs and other pollutants. Additionally, flagellar assembly and bacterial chemotaxis genes ranked high in the assembled genome of <i>Sphingomonas</i> sp., which might help it obtain a competitive advantage in the soil. The findings underscored the strategy of <i>Sphingomonas</i> to maintain dominance, enriched the understanding of PAH-degrading microorganisms in site soil, and provided references for the remediation of PAHs. |
| first_indexed | 2024-03-09T23:00:02Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:00:02Z |
| publishDate | 2022-05-01 |
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| series | Microorganisms |
| spelling | doaj.art-67d1958a6df74fd19d74d3c6fe8db6d82023-11-23T18:03:08ZengMDPI AGMicroorganisms2076-26072022-05-01106110910.3390/microorganisms10061109<i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking SitesMeng Zhou0Zishu Liu1Jiaqi Wang2Yuxiang Zhao3Baolan Hu4Department of Environmental Engineering, College of Environmental & Resources Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Environmental Engineering, College of Environmental & Resources Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Environmental Engineering, College of Environmental & Resources Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Environmental Engineering, College of Environmental & Resources Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Environmental Engineering, College of Environmental & Resources Sciences, Zhejiang University, Hangzhou 310058, ChinaPolycyclic aromatic hydrocarbons (PAHs) are organic pollutants widely present in industrial sites. Microbial degradation is an effective method of removing PAHs. The identification of microorganisms that have important ecological functions at the site is of great significance for PAH removal. We collected soil samples at three depths in the range of 0–100 cm at 70-day intervals at the coking site and explored the degradation of PAHs. We combined molecular ecology networking, metagenomics, and genome assembly to search for microorganisms that persist, dominate, and affect the microbial community construction in the degradation process and analyzed their adaptation strategies. The results showed that 15.78 mg/kg of PAHs naturally decayed, and 13.33 mg/kg of PAHs migrated from 30–100 cm to 0–30 cm in the soil. <i>Sphingomonas</i>, which occupied a niche advantage, was both the core and keystone microorganism, and its spatial distribution pattern and temporal change dynamics were consistent with those of PAHs. We assembled the genome of <i>Sphingomonas</i> sp., revealing its multiple potential for degrading PAHs and other pollutants. Additionally, flagellar assembly and bacterial chemotaxis genes ranked high in the assembled genome of <i>Sphingomonas</i> sp., which might help it obtain a competitive advantage in the soil. The findings underscored the strategy of <i>Sphingomonas</i> to maintain dominance, enriched the understanding of PAH-degrading microorganisms in site soil, and provided references for the remediation of PAHs.https://www.mdpi.com/2076-2607/10/6/1109soilPAH degradation<i>Sphingomonas</i>microorganismsmetagenomics |
| spellingShingle | Meng Zhou Zishu Liu Jiaqi Wang Yuxiang Zhao Baolan Hu <i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites Microorganisms soil PAH degradation <i>Sphingomonas</i> microorganisms metagenomics |
| title | <i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites |
| title_full | <i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites |
| title_fullStr | <i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites |
| title_full_unstemmed | <i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites |
| title_short | <i>Sphingomonas</i> Relies on Chemotaxis to Degrade Polycyclic Aromatic Hydrocarbons and Maintain Dominance in Coking Sites |
| title_sort | i sphingomonas i relies on chemotaxis to degrade polycyclic aromatic hydrocarbons and maintain dominance in coking sites |
| topic | soil PAH degradation <i>Sphingomonas</i> microorganisms metagenomics |
| url | https://www.mdpi.com/2076-2607/10/6/1109 |
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