ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED
Alpha-lactalbumin (α-La) is a major milk protein essential for the biosynthesis of lactose at the level of mammary glands. α-La directly influences the quality and the volume of the milk since it is directly involved in the lactose synthesis (Ashwell et al., 1997). The PCR-RFLP test was performed to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-11-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1728 |
| _version_ | 1827289344632684544 |
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| author | R.A. VĂTĂŞESCU-BALCAN S.E. GEORGESCU MARIA ADINA MANEA ANCA DINISCHIOTU C.D. TESIO MARIETA COSTACHE |
| author_facet | R.A. VĂTĂŞESCU-BALCAN S.E. GEORGESCU MARIA ADINA MANEA ANCA DINISCHIOTU C.D. TESIO MARIETA COSTACHE |
| author_sort | R.A. VĂTĂŞESCU-BALCAN |
| collection | DOAJ |
| description | Alpha-lactalbumin (α-La) is a major milk protein essential for the biosynthesis of
lactose at the level of mammary glands. α-La directly influences the quality and the
volume of the milk since it is directly involved in the lactose synthesis (Ashwell et al.,
1997). The PCR-RFLP test was performed to distinguish the different alleles in a
population of Romanian Black Spotted cattle, a dairy breed. Genetic polymorphism
was detected by digestion with the endonuclease MnlI, followed by electrophoresis
in high resolution agarose gel stained with ethidium bromide. Sixty DNA samples
from Romanian Black Spotted breed were analyzed for A and B variants. The PCRRFLP test makes feasible the inclusion of α-La genotypes in breeding plans and
cattle selection. |
| first_indexed | 2024-04-24T11:48:01Z |
| format | Article |
| id | doaj.art-67d4af1271f448659aa961324214db44 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:48:01Z |
| publishDate | 2023-11-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-67d4af1271f448659aa961324214db442024-04-09T08:50:27ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-11-014111691691728ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREEDR.A. VĂTĂŞESCU-BALCAN0S.E. GEORGESCU1MARIA ADINA MANEA2ANCA DINISCHIOTU3C.D. TESIO4MARIETA COSTACHE5University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest University of Bucharest, Faculty of Biology, Molecular Biology Center, Bucharest Alpha-lactalbumin (α-La) is a major milk protein essential for the biosynthesis of lactose at the level of mammary glands. α-La directly influences the quality and the volume of the milk since it is directly involved in the lactose synthesis (Ashwell et al., 1997). The PCR-RFLP test was performed to distinguish the different alleles in a population of Romanian Black Spotted cattle, a dairy breed. Genetic polymorphism was detected by digestion with the endonuclease MnlI, followed by electrophoresis in high resolution agarose gel stained with ethidium bromide. Sixty DNA samples from Romanian Black Spotted breed were analyzed for A and B variants. The PCRRFLP test makes feasible the inclusion of α-La genotypes in breeding plans and cattle selection.https://spasb.ro/index.php/public_html/article/view/1728alpha-lactalbumingenetic polymorphismpcrrflpcattle breed |
| spellingShingle | R.A. VĂTĂŞESCU-BALCAN S.E. GEORGESCU MARIA ADINA MANEA ANCA DINISCHIOTU C.D. TESIO MARIETA COSTACHE ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED Scientific Papers Animal Science and Biotechnologies alpha-lactalbumin genetic polymorphism pcr rflp cattle breed |
| title | ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED |
| title_full | ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED |
| title_fullStr | ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED |
| title_full_unstemmed | ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED |
| title_short | ALPHA-LACTALBUMIN GENOTYPES IDENTIFICATION IN ROMANIAN BLACK SPOTTED CATTLE BREED |
| title_sort | alpha lactalbumin genotypes identification in romanian black spotted cattle breed |
| topic | alpha-lactalbumin genetic polymorphism pcr rflp cattle breed |
| url | https://spasb.ro/index.php/public_html/article/view/1728 |
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