Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades
Abstract Background Diabetic neuropathy (DN) is a frequent and debilitating manifestation of diabetes mellitus, to which there are no effective therapeutic approaches. Mesenchymal stem/stromal cells (MSC) have a great potential for the treatment of this syndrome, possibly through regenerative action...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Journal of Neuroinflammation |
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| Online Access: | http://link.springer.com/article/10.1186/s12974-018-1224-3 |
| _version_ | 1828850536758116352 |
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| author | Afrânio Ferreira Evangelista Marcos André Vannier-Santos Gessica Sabrina de Assis Silva Daniela Nascimento Silva Paulo José Lima Juiz Carolina Kymie Vasques Nonaka Ricardo Ribeiro dos Santos Milena Botelho Pereira Soares Cristiane Flora Villarreal |
| author_facet | Afrânio Ferreira Evangelista Marcos André Vannier-Santos Gessica Sabrina de Assis Silva Daniela Nascimento Silva Paulo José Lima Juiz Carolina Kymie Vasques Nonaka Ricardo Ribeiro dos Santos Milena Botelho Pereira Soares Cristiane Flora Villarreal |
| author_sort | Afrânio Ferreira Evangelista |
| collection | DOAJ |
| description | Abstract Background Diabetic neuropathy (DN) is a frequent and debilitating manifestation of diabetes mellitus, to which there are no effective therapeutic approaches. Mesenchymal stem/stromal cells (MSC) have a great potential for the treatment of this syndrome, possibly through regenerative actions on peripheral nerves. Here, we evaluated the therapeutic effects of MSC on spinal neuroinflammation, as well as on ultrastructural aspects of the peripheral nerve in DN-associated sensorial dysfunction. Methods C57Bl/6 mice were treated with bone marrow-derived MSC (1 × 106), conditioned medium from MSC cultures (CM-MSC) or vehicle by endovenous route following the onset of streptozotocin (STZ)-induced diabetes. Paw mechanical and thermal nociceptive thresholds were evaluated by using von Frey filaments and Hargreaves test, respectively. Morphological and morphometric analysis of the sciatic nerve was performed by light microscopy and transmission electron microscopy. Mediators and markers of neuroinflammation in the spinal cord were measured by radioimmunoassay, real-time PCR, and immunofluorescence analyses. Results Diabetic mice presented behavioral signs of sensory neuropathy, mechanical allodynia, and heat hypoalgesia, which were completely reversed by a single administration of MSC or CM-MSC. The ultrastructural analysis of the sciatic nerve showed that diabetic mice exhibited morphological and morphometric alterations, considered hallmarks of DN, such as degenerative changes in axons and myelin sheath, and reduced area and density of unmyelinated fibers. In MSC-treated mice, these structural alterations were markedly less commonly observed and/or less pronounced. Moreover, MSC transplantation inhibited multiple parameters of spinal neuroinflammation found in diabetic mice, causing the reduction of activated astrocytes and microglia, oxidative stress signals, galectin-3, IL-1β, and TNF-α production. Conversely, MSC increased the levels of anti-inflammatory cytokines, IL-10, and TGF-β. Conclusions The present study described the modulatory effects of MSC on spinal cord neuroinflammation in diabetic mice, suggesting new mechanisms by which MSC can improve DN. |
| first_indexed | 2024-12-12T23:13:52Z |
| format | Article |
| id | doaj.art-67dae5e1229c44cc88915d764ca2daf6 |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-12T23:13:52Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-67dae5e1229c44cc88915d764ca2daf62022-12-22T00:08:31ZengBMCJournal of Neuroinflammation1742-20942018-06-0115111710.1186/s12974-018-1224-3Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascadesAfrânio Ferreira Evangelista0Marcos André Vannier-Santos1Gessica Sabrina de Assis Silva2Daniela Nascimento Silva3Paulo José Lima Juiz4Carolina Kymie Vasques Nonaka5Ricardo Ribeiro dos Santos6Milena Botelho Pereira Soares7Cristiane Flora Villarreal8Gonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZOswaldo Cruz Institute, Oswaldo Cruz Foundation-IOC-FIOCRUZPharmacy College, Federal University of BahiaCenter of Biotechnology and Cell Therapy, São Rafael HospitalFederal University of Recôncavo of BahiaCenter of Biotechnology and Cell Therapy, São Rafael HospitalCenter of Biotechnology and Cell Therapy, São Rafael HospitalGonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZGonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZAbstract Background Diabetic neuropathy (DN) is a frequent and debilitating manifestation of diabetes mellitus, to which there are no effective therapeutic approaches. Mesenchymal stem/stromal cells (MSC) have a great potential for the treatment of this syndrome, possibly through regenerative actions on peripheral nerves. Here, we evaluated the therapeutic effects of MSC on spinal neuroinflammation, as well as on ultrastructural aspects of the peripheral nerve in DN-associated sensorial dysfunction. Methods C57Bl/6 mice were treated with bone marrow-derived MSC (1 × 106), conditioned medium from MSC cultures (CM-MSC) or vehicle by endovenous route following the onset of streptozotocin (STZ)-induced diabetes. Paw mechanical and thermal nociceptive thresholds were evaluated by using von Frey filaments and Hargreaves test, respectively. Morphological and morphometric analysis of the sciatic nerve was performed by light microscopy and transmission electron microscopy. Mediators and markers of neuroinflammation in the spinal cord were measured by radioimmunoassay, real-time PCR, and immunofluorescence analyses. Results Diabetic mice presented behavioral signs of sensory neuropathy, mechanical allodynia, and heat hypoalgesia, which were completely reversed by a single administration of MSC or CM-MSC. The ultrastructural analysis of the sciatic nerve showed that diabetic mice exhibited morphological and morphometric alterations, considered hallmarks of DN, such as degenerative changes in axons and myelin sheath, and reduced area and density of unmyelinated fibers. In MSC-treated mice, these structural alterations were markedly less commonly observed and/or less pronounced. Moreover, MSC transplantation inhibited multiple parameters of spinal neuroinflammation found in diabetic mice, causing the reduction of activated astrocytes and microglia, oxidative stress signals, galectin-3, IL-1β, and TNF-α production. Conversely, MSC increased the levels of anti-inflammatory cytokines, IL-10, and TGF-β. Conclusions The present study described the modulatory effects of MSC on spinal cord neuroinflammation in diabetic mice, suggesting new mechanisms by which MSC can improve DN.http://link.springer.com/article/10.1186/s12974-018-1224-3Stem cellsSensory neuropathyDiabetesSpinal cordNeuroinflammationGalectin-3 |
| spellingShingle | Afrânio Ferreira Evangelista Marcos André Vannier-Santos Gessica Sabrina de Assis Silva Daniela Nascimento Silva Paulo José Lima Juiz Carolina Kymie Vasques Nonaka Ricardo Ribeiro dos Santos Milena Botelho Pereira Soares Cristiane Flora Villarreal Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades Journal of Neuroinflammation Stem cells Sensory neuropathy Diabetes Spinal cord Neuroinflammation Galectin-3 |
| title | Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades |
| title_full | Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades |
| title_fullStr | Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades |
| title_full_unstemmed | Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades |
| title_short | Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades |
| title_sort | bone marrow derived mesenchymal stem stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades |
| topic | Stem cells Sensory neuropathy Diabetes Spinal cord Neuroinflammation Galectin-3 |
| url | http://link.springer.com/article/10.1186/s12974-018-1224-3 |
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