Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells
CD34 is a cell surface glycoprotein expressed by hematopoietic progenitors and endothelial cells. It is widely used in the clinic for isolation of human hematopoietic stem cells. In recent years large animals are gaining increasing importance in biomedical research for the study and therapy of human...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2010-02-01
|
| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/255 |
| _version_ | 1828484677491490816 |
|---|---|
| author | Maria Dattena Elisabetta Antuofermo Roberto Manetti Paola Nicolussi Piero Bonelli |
| author_facet | Maria Dattena Elisabetta Antuofermo Roberto Manetti Paola Nicolussi Piero Bonelli |
| author_sort | Maria Dattena |
| collection | DOAJ |
| description | CD34 is a cell surface glycoprotein expressed by hematopoietic progenitors and endothelial cells. It is widely used in the clinic for isolation of human hematopoietic stem cells. In recent years large animals are gaining increasing importance in biomedical research for the study and therapy of human diseases. Sheep has proved to be an useful experimental model for preclinical trials in transplantation procedures. Unfortunately, the lack of specie-specific monoclonal antibodies (MABS) recognizing hemopoietic progenitor cells hampers the use of this animal in experimental hematology. The aim of this paper was to determine whether commercial monoclonal antibodies specific for human CD34 molecule could cross-react with hematopoietic progenitor cells (HPC) present in sheep umbilical cord blood (UCB). Six antihuman CD34 MABS, recognizing the three different epitope classes, were tested in flow cytometry on purified mononuclear cells (MNC) isolated from cord blood of both species. None of the MABS used in this trial seemed to be able to identify HPC from sheep UCB. These data suggest that the panel of monoclonal antibodies used for cross reactivity detection has to be expanded with recently produced reagents. Further studies should be directed towards the production of ovine specific anti CD34 MABS. |
| first_indexed | 2024-12-11T08:59:49Z |
| format | Article |
| id | doaj.art-67daf5f440274744bebc9d0325d31cc5 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-11T08:59:49Z |
| publishDate | 2010-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-67daf5f440274744bebc9d0325d31cc52022-12-22T01:13:48ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-02-0191e6e610.4081/ijas.2010.e6Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cellsMaria DattenaElisabetta AntuofermoRoberto ManettiPaola NicolussiPiero BonelliCD34 is a cell surface glycoprotein expressed by hematopoietic progenitors and endothelial cells. It is widely used in the clinic for isolation of human hematopoietic stem cells. In recent years large animals are gaining increasing importance in biomedical research for the study and therapy of human diseases. Sheep has proved to be an useful experimental model for preclinical trials in transplantation procedures. Unfortunately, the lack of specie-specific monoclonal antibodies (MABS) recognizing hemopoietic progenitor cells hampers the use of this animal in experimental hematology. The aim of this paper was to determine whether commercial monoclonal antibodies specific for human CD34 molecule could cross-react with hematopoietic progenitor cells (HPC) present in sheep umbilical cord blood (UCB). Six antihuman CD34 MABS, recognizing the three different epitope classes, were tested in flow cytometry on purified mononuclear cells (MNC) isolated from cord blood of both species. None of the MABS used in this trial seemed to be able to identify HPC from sheep UCB. These data suggest that the panel of monoclonal antibodies used for cross reactivity detection has to be expanded with recently produced reagents. Further studies should be directed towards the production of ovine specific anti CD34 MABS.http://www.aspajournal.it/index.php/ijas/article/view/255CD 34, Hemopoietic progenitor cells, Cross-reactivity, Sheep. |
| spellingShingle | Maria Dattena Elisabetta Antuofermo Roberto Manetti Paola Nicolussi Piero Bonelli Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells Italian Journal of Animal Science CD 34, Hemopoietic progenitor cells, Cross-reactivity, Sheep. |
| title | Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells |
| title_full | Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells |
| title_fullStr | Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells |
| title_full_unstemmed | Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells |
| title_short | Monoclonal antibodies against human CD34 antigens do not cross-react with ovine umbilical cord blood cells |
| title_sort | monoclonal antibodies against human cd34 antigens do not cross react with ovine umbilical cord blood cells |
| topic | CD 34, Hemopoietic progenitor cells, Cross-reactivity, Sheep. |
| url | http://www.aspajournal.it/index.php/ijas/article/view/255 |
| work_keys_str_mv | AT mariadattena monoclonalantibodiesagainsthumancd34antigensdonotcrossreactwithovineumbilicalcordbloodcells AT elisabettaantuofermo monoclonalantibodiesagainsthumancd34antigensdonotcrossreactwithovineumbilicalcordbloodcells AT robertomanetti monoclonalantibodiesagainsthumancd34antigensdonotcrossreactwithovineumbilicalcordbloodcells AT paolanicolussi monoclonalantibodiesagainsthumancd34antigensdonotcrossreactwithovineumbilicalcordbloodcells AT pierobonelli monoclonalantibodiesagainsthumancd34antigensdonotcrossreactwithovineumbilicalcordbloodcells |