The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay
Abstract Edible bird's nest (EBN) is a traditional food which was nourishing and functional. Particularly, there is the epidermal growth factor (EGF) in EBN, which is thought to play an important role in promoting skin repair. However, the type and content of EGF in EBN were not determined yet....
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2023-06-01
|
| Series: | Applied Biological Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13765-023-00794-2 |
| _version_ | 1797806509095124992 |
|---|---|
| author | Weijuan Bai Fenghong Deng Xiaojiang Zhang Yanping Han Yue’e Xiao Nan Wang Xuncai Liu Qunyan Fan Baozhong Guo |
| author_facet | Weijuan Bai Fenghong Deng Xiaojiang Zhang Yanping Han Yue’e Xiao Nan Wang Xuncai Liu Qunyan Fan Baozhong Guo |
| author_sort | Weijuan Bai |
| collection | DOAJ |
| description | Abstract Edible bird's nest (EBN) is a traditional food which was nourishing and functional. Particularly, there is the epidermal growth factor (EGF) in EBN, which is thought to play an important role in promoting skin repair. However, the type and content of EGF in EBN were not determined yet. In this study, the type of EGF in EBN was identified as bird EGF by enzyme-linked immunosorbent assay and this method was validated to be accurate and precise. Moreover, it was found that the content of EGF in raw-unclean EBN, raw-clean EBN and stewed EBN was 3000 pg/g–4000 pg/g and there were no significant differences, which suggested that the batches, origins, forms, stewing temperatures and stewing times of EBN had no effect on the content of EGF in EBN. However, it was due to that enzyme destroyed the primary structure of EGF, the EGF content of neutral protease and trypsin hydrolysates of EBN was lower than that of flavor enzymes, alkaline protease and pepsin hydrolysates of EGF. This study was the first to determine the type and content of EGF in EBN, and provided a theoretical basis for the selection and processing of EBN and using EBN as a source of EGF. |
| first_indexed | 2024-03-13T06:08:24Z |
| format | Article |
| id | doaj.art-67dbe1cdb43e4c93973ea7da5227195c |
| institution | Directory Open Access Journal |
| issn | 2468-0842 |
| language | English |
| last_indexed | 2024-03-13T06:08:24Z |
| publishDate | 2023-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Applied Biological Chemistry |
| spelling | doaj.art-67dbe1cdb43e4c93973ea7da5227195c2023-06-11T11:23:19ZengSpringerOpenApplied Biological Chemistry2468-08422023-06-016611910.1186/s13765-023-00794-2The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assayWeijuan Bai0Fenghong Deng1Xiaojiang Zhang2Yanping Han3Yue’e Xiao4Nan Wang5Xuncai Liu6Qunyan Fan7Baozhong Guo8Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Research Institute of Bird’s Nest, Xiamen Yan Palace Seelong Food Co., Ltd.Abstract Edible bird's nest (EBN) is a traditional food which was nourishing and functional. Particularly, there is the epidermal growth factor (EGF) in EBN, which is thought to play an important role in promoting skin repair. However, the type and content of EGF in EBN were not determined yet. In this study, the type of EGF in EBN was identified as bird EGF by enzyme-linked immunosorbent assay and this method was validated to be accurate and precise. Moreover, it was found that the content of EGF in raw-unclean EBN, raw-clean EBN and stewed EBN was 3000 pg/g–4000 pg/g and there were no significant differences, which suggested that the batches, origins, forms, stewing temperatures and stewing times of EBN had no effect on the content of EGF in EBN. However, it was due to that enzyme destroyed the primary structure of EGF, the EGF content of neutral protease and trypsin hydrolysates of EBN was lower than that of flavor enzymes, alkaline protease and pepsin hydrolysates of EGF. This study was the first to determine the type and content of EGF in EBN, and provided a theoretical basis for the selection and processing of EBN and using EBN as a source of EGF.https://doi.org/10.1186/s13765-023-00794-2Edible bird's nestEpidermal growth factorContent |
| spellingShingle | Weijuan Bai Fenghong Deng Xiaojiang Zhang Yanping Han Yue’e Xiao Nan Wang Xuncai Liu Qunyan Fan Baozhong Guo The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay Applied Biological Chemistry Edible bird's nest Epidermal growth factor Content |
| title | The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay |
| title_full | The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay |
| title_fullStr | The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay |
| title_full_unstemmed | The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay |
| title_short | The determination of epidermal growth factor in Edible bird's nest by enzyme-linked immunosorbent assay |
| title_sort | determination of epidermal growth factor in edible bird s nest by enzyme linked immunosorbent assay |
| topic | Edible bird's nest Epidermal growth factor Content |
| url | https://doi.org/10.1186/s13765-023-00794-2 |
| work_keys_str_mv | AT weijuanbai thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT fenghongdeng thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT xiaojiangzhang thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT yanpinghan thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT yueexiao thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT nanwang thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT xuncailiu thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT qunyanfan thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT baozhongguo thedeterminationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT weijuanbai determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT fenghongdeng determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT xiaojiangzhang determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT yanpinghan determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT yueexiao determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT nanwang determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT xuncailiu determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT qunyanfan determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay AT baozhongguo determinationofepidermalgrowthfactorinediblebirdsnestbyenzymelinkedimmunosorbentassay |