ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children
Abstract Members of the highly polymorphic Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of infected erythrocytes (IEs) are important virulence factors, which mediate vascular adhesion of IEs via endothelial host receptors and are targets of naturally...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16305-0 |
| _version_ | 1811222999849762816 |
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| author | Jennifer Suurbaar Azizath Moussiliou Rachida Tahar Rebecca W. Olsen Yvonne Adams Nanna Dalgaard Eric K. Baafour Selorme Adukpo Lars Hviid Kwadwo A. Kusi Jules Alao Michael F. Ofori Nicaise T. Ndam Anja R. Jensen |
| author_facet | Jennifer Suurbaar Azizath Moussiliou Rachida Tahar Rebecca W. Olsen Yvonne Adams Nanna Dalgaard Eric K. Baafour Selorme Adukpo Lars Hviid Kwadwo A. Kusi Jules Alao Michael F. Ofori Nicaise T. Ndam Anja R. Jensen |
| author_sort | Jennifer Suurbaar |
| collection | DOAJ |
| description | Abstract Members of the highly polymorphic Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of infected erythrocytes (IEs) are important virulence factors, which mediate vascular adhesion of IEs via endothelial host receptors and are targets of naturally acquired immunity. The PfEMP1 family can be divided into clinically relevant subgroups, of which some bind intercellular adhesion molecule 1 (ICAM-1). While the acquisition of IgG specific for ICAM-1-binding DBLβ domains is known to differ between PfEMP1 groups, its ability to induce antibody-dependent cellular phagocytosis (ADCP) is unclear. We therefore measured plasma levels of DBLβ-specific IgG, the ability of such IgG to inhibit PfEMP1-binding to ICAM-1, and its ability to opsonize IEs for ADCP, using plasma from Beninese children with severe (SM) or uncomplicated malaria (UM). IgG specific for DBLβ from group A and B ICAM-1-binding PfEMP1 were dominated by IgG1 and IgG3, and were similar in SM and UM. However, levels of plasma IgG inhibiting ICAM-1-binding of group A DBLβ of PFD1235w was significantly higher in children with UM than SM, and acute UM plasma induced a higher ADCP response than acute SM plasma. |
| first_indexed | 2024-04-12T08:24:24Z |
| format | Article |
| id | doaj.art-67dd0492bb8949eaaa58bf614d003c41 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T08:24:24Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-67dd0492bb8949eaaa58bf614d003c412022-12-22T03:40:26ZengNature PortfolioScientific Reports2045-23222022-07-0112111210.1038/s41598-022-16305-0ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese childrenJennifer Suurbaar0Azizath Moussiliou1Rachida Tahar2Rebecca W. Olsen3Yvonne Adams4Nanna Dalgaard5Eric K. Baafour6Selorme Adukpo7Lars Hviid8Kwadwo A. Kusi9Jules Alao10Michael F. Ofori11Nicaise T. Ndam12Anja R. Jensen13Department of Immunology, Noguchi Memorial Institute for Medical Research, University of GhanaUniversité de Paris Cité, MERIT, IRDUniversité de Paris Cité, MERIT, IRDCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of CopenhagenCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of CopenhagenCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Immunology, Noguchi Memorial Institute for Medical Research, University of GhanaDepartment of Pharmaceutics and Microbiology, School of Pharmacy, University of GhanaCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Immunology, Noguchi Memorial Institute for Medical Research, University of GhanaPaediatric Department, Mother and Child University and Hospital Center (CHUMEL)Department of Immunology, Noguchi Memorial Institute for Medical Research, University of GhanaUniversité de Paris Cité, MERIT, IRDCentre for Medical Parasitology at Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of CopenhagenAbstract Members of the highly polymorphic Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of infected erythrocytes (IEs) are important virulence factors, which mediate vascular adhesion of IEs via endothelial host receptors and are targets of naturally acquired immunity. The PfEMP1 family can be divided into clinically relevant subgroups, of which some bind intercellular adhesion molecule 1 (ICAM-1). While the acquisition of IgG specific for ICAM-1-binding DBLβ domains is known to differ between PfEMP1 groups, its ability to induce antibody-dependent cellular phagocytosis (ADCP) is unclear. We therefore measured plasma levels of DBLβ-specific IgG, the ability of such IgG to inhibit PfEMP1-binding to ICAM-1, and its ability to opsonize IEs for ADCP, using plasma from Beninese children with severe (SM) or uncomplicated malaria (UM). IgG specific for DBLβ from group A and B ICAM-1-binding PfEMP1 were dominated by IgG1 and IgG3, and were similar in SM and UM. However, levels of plasma IgG inhibiting ICAM-1-binding of group A DBLβ of PFD1235w was significantly higher in children with UM than SM, and acute UM plasma induced a higher ADCP response than acute SM plasma.https://doi.org/10.1038/s41598-022-16305-0 |
| spellingShingle | Jennifer Suurbaar Azizath Moussiliou Rachida Tahar Rebecca W. Olsen Yvonne Adams Nanna Dalgaard Eric K. Baafour Selorme Adukpo Lars Hviid Kwadwo A. Kusi Jules Alao Michael F. Ofori Nicaise T. Ndam Anja R. Jensen ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children Scientific Reports |
| title | ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children |
| title_full | ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children |
| title_fullStr | ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children |
| title_full_unstemmed | ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children |
| title_short | ICAM-1-binding Plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic-phagocytosis IgG responses in Beninese children |
| title_sort | icam 1 binding plasmodium falciparum erythrocyte membrane protein 1 variants elicits opsonic phagocytosis igg responses in beninese children |
| url | https://doi.org/10.1038/s41598-022-16305-0 |
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