Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization
Background/Aims: Transplantation of mesenchymal stem cells (MSCs) improves post-injury cardiac muscle repair using ill-defined mechanisms. Recently, we have shown that production and secretion of placental growth factor (PLGF) by MSCs play a critical role in the MSCs-mediated post-injury cardiac mus...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2015-06-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/430269 |
| _version_ | 1819141848241799168 |
|---|---|
| author | Jianfeng Zhang Anqing Chen Yicheng Wu Qiang Zhao |
| author_facet | Jianfeng Zhang Anqing Chen Yicheng Wu Qiang Zhao |
| author_sort | Jianfeng Zhang |
| collection | DOAJ |
| description | Background/Aims: Transplantation of mesenchymal stem cells (MSCs) improves post-injury cardiac muscle repair using ill-defined mechanisms. Recently, we have shown that production and secretion of placental growth factor (PLGF) by MSCs play a critical role in the MSCs-mediated post-injury cardiac muscle repair. In this study, we addressed the underlying molecular mechanisms, focusing specifically on the interactions between MSCs, macrophages and endothelial cells. Methods: We isolated macrophages (BM-MΦ) from mouse bone-marrow derived cells based on F4/80 expression by flow cytometry. BM-MΦ were treated with different doses of PLGF. Cell number was analyzed by a MTT assay. Macrophage polarization was examined based on CD206 expression by flow cytometry. PLGF levels in macrophage subpopulations were analyzed by RT-qPCR and ELISA. Effects of macrophages on vascularization were evaluated by a collagen gel assay using Human umbilical vein endothelial cells (HUVECs) co-cultured with PLGF-treated macrophages. Results: PLGF did not increase macrophage number, but dose-dependently polarized macrophages into a M2 subpopulation. M2 macrophages expressed high levels of PLGF. PLGF-polarized M2 macrophages significantly increased tubular structures in the collagen gel assay. Conclusion: Our data suggest that MSCs-derived PLGF may induce macrophage polarization into a M2 subpopulation, which in turn releases more PLGF to promote local neovascularization for augmenting post-injury cardiac muscle repair. This study thus sheds novel light on the role of PLGF in cardiac muscle regeneration. |
| first_indexed | 2024-12-22T12:00:58Z |
| format | Article |
| id | doaj.art-67e152eb89db4b1c83bfd4c58166fce7 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-22T12:00:58Z |
| publishDate | 2015-06-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-67e152eb89db4b1c83bfd4c58166fce72022-12-21T18:26:37ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782015-06-0136394795510.1159/000430269430269Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced NeovascularizationJianfeng ZhangAnqing ChenYicheng WuQiang ZhaoBackground/Aims: Transplantation of mesenchymal stem cells (MSCs) improves post-injury cardiac muscle repair using ill-defined mechanisms. Recently, we have shown that production and secretion of placental growth factor (PLGF) by MSCs play a critical role in the MSCs-mediated post-injury cardiac muscle repair. In this study, we addressed the underlying molecular mechanisms, focusing specifically on the interactions between MSCs, macrophages and endothelial cells. Methods: We isolated macrophages (BM-MΦ) from mouse bone-marrow derived cells based on F4/80 expression by flow cytometry. BM-MΦ were treated with different doses of PLGF. Cell number was analyzed by a MTT assay. Macrophage polarization was examined based on CD206 expression by flow cytometry. PLGF levels in macrophage subpopulations were analyzed by RT-qPCR and ELISA. Effects of macrophages on vascularization were evaluated by a collagen gel assay using Human umbilical vein endothelial cells (HUVECs) co-cultured with PLGF-treated macrophages. Results: PLGF did not increase macrophage number, but dose-dependently polarized macrophages into a M2 subpopulation. M2 macrophages expressed high levels of PLGF. PLGF-polarized M2 macrophages significantly increased tubular structures in the collagen gel assay. Conclusion: Our data suggest that MSCs-derived PLGF may induce macrophage polarization into a M2 subpopulation, which in turn releases more PLGF to promote local neovascularization for augmenting post-injury cardiac muscle repair. This study thus sheds novel light on the role of PLGF in cardiac muscle regeneration.http://www.karger.com/Article/FullText/430269Mesenchymal stem cells (MSCs)Cardiac muscle repairPlacental growth factor (PLGF)Macrophage polarization |
| spellingShingle | Jianfeng Zhang Anqing Chen Yicheng Wu Qiang Zhao Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization Cellular Physiology and Biochemistry Mesenchymal stem cells (MSCs) Cardiac muscle repair Placental growth factor (PLGF) Macrophage polarization |
| title | Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization |
| title_full | Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization |
| title_fullStr | Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization |
| title_full_unstemmed | Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization |
| title_short | Placental Growth Factor Promotes Cardiac Muscle Repair via Enhanced Neovascularization |
| title_sort | placental growth factor promotes cardiac muscle repair via enhanced neovascularization |
| topic | Mesenchymal stem cells (MSCs) Cardiac muscle repair Placental growth factor (PLGF) Macrophage polarization |
| url | http://www.karger.com/Article/FullText/430269 |
| work_keys_str_mv | AT jianfengzhang placentalgrowthfactorpromotescardiacmusclerepairviaenhancedneovascularization AT anqingchen placentalgrowthfactorpromotescardiacmusclerepairviaenhancedneovascularization AT yichengwu placentalgrowthfactorpromotescardiacmusclerepairviaenhancedneovascularization AT qiangzhao placentalgrowthfactorpromotescardiacmusclerepairviaenhancedneovascularization |