Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing
Although significant improvements have been made in the treatment of pancreatic cancer, its prognosis remains poor with an overall 5-year survival rate of less than 10%. New experimental approaches are necessary to develop novel therapeutics. In this study, the investigation of pancreatic cancer tis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Organoids |
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| Online Access: | https://www.mdpi.com/2674-1172/3/1/1 |
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| author | Andreas Ettner-Sitter Agata Montagner Jonas Kuenzel Kathrin Brackmann Maximilian Schäfer Robert Schober Florian Weber Thiha Aung Christina Hackl Silke Haerteis |
| author_facet | Andreas Ettner-Sitter Agata Montagner Jonas Kuenzel Kathrin Brackmann Maximilian Schäfer Robert Schober Florian Weber Thiha Aung Christina Hackl Silke Haerteis |
| author_sort | Andreas Ettner-Sitter |
| collection | DOAJ |
| description | Although significant improvements have been made in the treatment of pancreatic cancer, its prognosis remains poor with an overall 5-year survival rate of less than 10%. New experimental approaches are necessary to develop novel therapeutics. In this study, the investigation of pancreatic cancer tissue growth in the chorioallantoic membrane (CAM) model and the subsequent use of indocyanine green (ICG) injections for the verification of intratumoral perfusion was conducted. ICG was injected into the CAM vasculature to visualize the perfusion of the tumor tissue. The presence of metastasis was investigated through PCR for the human-specific ALU element in the liver of the chicken embryo. Additionally, the usage of cryopreserved pancreatic tumors was established. Intratumoral perfusion of tumor tissue on the CAM was observed in recently obtained and cryopreserved tumors. ALU-PCR detected metastasis in the chick embryos’ livers. After cryopreservation, the tissue was still vital, and the xenografts generated from these tumors resembled the histological features of the primary tumor. This methodology represents the proof of principle for intravenous drug testing of pancreatic cancer in the CAM model. The cryopreserved tumors can be used for testing novel therapeutics and can be integrated into the molecular tumor board, facilitating personalized tumor treatment. |
| first_indexed | 2024-04-24T17:56:05Z |
| format | Article |
| id | doaj.art-67e424d9d4014ab49bdae919f98ea6e9 |
| institution | Directory Open Access Journal |
| issn | 2674-1172 |
| language | English |
| last_indexed | 2024-04-24T17:56:05Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Organoids |
| spelling | doaj.art-67e424d9d4014ab49bdae919f98ea6e92024-03-27T13:58:38ZengMDPI AGOrganoids2674-11722024-01-013111710.3390/organoids3010001Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug TestingAndreas Ettner-Sitter0Agata Montagner1Jonas Kuenzel2Kathrin Brackmann3Maximilian Schäfer4Robert Schober5Florian Weber6Thiha Aung7Christina Hackl8Silke Haerteis9Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, GermanyInstitute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, GermanyInstitute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, GermanyDepartment of Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyInstitute for Digital Communications, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, GermanyInstitute for Digital Communications, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, GermanyInstitute of Pathology, University of Regensburg, 93053 Regensburg, GermanyInstitute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, GermanyDepartment of Surgery, University Hospital Regensburg, 93053 Regensburg, GermanyInstitute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, GermanyAlthough significant improvements have been made in the treatment of pancreatic cancer, its prognosis remains poor with an overall 5-year survival rate of less than 10%. New experimental approaches are necessary to develop novel therapeutics. In this study, the investigation of pancreatic cancer tissue growth in the chorioallantoic membrane (CAM) model and the subsequent use of indocyanine green (ICG) injections for the verification of intratumoral perfusion was conducted. ICG was injected into the CAM vasculature to visualize the perfusion of the tumor tissue. The presence of metastasis was investigated through PCR for the human-specific ALU element in the liver of the chicken embryo. Additionally, the usage of cryopreserved pancreatic tumors was established. Intratumoral perfusion of tumor tissue on the CAM was observed in recently obtained and cryopreserved tumors. ALU-PCR detected metastasis in the chick embryos’ livers. After cryopreservation, the tissue was still vital, and the xenografts generated from these tumors resembled the histological features of the primary tumor. This methodology represents the proof of principle for intravenous drug testing of pancreatic cancer in the CAM model. The cryopreserved tumors can be used for testing novel therapeutics and can be integrated into the molecular tumor board, facilitating personalized tumor treatment.https://www.mdpi.com/2674-1172/3/1/1indocyanine greenpancreatic cancercryopreservationCAM model3D in vivo tumor model |
| spellingShingle | Andreas Ettner-Sitter Agata Montagner Jonas Kuenzel Kathrin Brackmann Maximilian Schäfer Robert Schober Florian Weber Thiha Aung Christina Hackl Silke Haerteis Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing Organoids indocyanine green pancreatic cancer cryopreservation CAM model 3D in vivo tumor model |
| title | Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing |
| title_full | Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing |
| title_fullStr | Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing |
| title_full_unstemmed | Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing |
| title_short | Visualization of Vascular Perfusion of Human Pancreatic Cancer Tissue in the CAM Model and Its Impact on Future Personalized Drug Testing |
| title_sort | visualization of vascular perfusion of human pancreatic cancer tissue in the cam model and its impact on future personalized drug testing |
| topic | indocyanine green pancreatic cancer cryopreservation CAM model 3D in vivo tumor model |
| url | https://www.mdpi.com/2674-1172/3/1/1 |
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