The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning
The study investigated the effect of grape seed (GS) meal, aflatoxin (AFB1), or their combination on the large intestine microbiota of weanling piglets. Twenty-four piglets were allocated into four groups based on diet composition: (1) Control group; (2) AFB1 (320 g/kg feed) group; (3) GS group (8%...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-01-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/11/1/25 |
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| author | Iulian A. Grosu Gina C. Pistol Ionelia Taranu Daniela E. Marin |
| author_facet | Iulian A. Grosu Gina C. Pistol Ionelia Taranu Daniela E. Marin |
| author_sort | Iulian A. Grosu |
| collection | DOAJ |
| description | The study investigated the effect of grape seed (GS) meal, aflatoxin (AFB1), or their combination on the large intestine microbiota of weanling piglets. Twenty-four piglets were allocated into four groups based on diet composition: (1) Control group; (2) AFB1 (320 g/kg feed) group; (3) GS group (8% inclusion in the diet); (4) AFB1 + GS group. After 30 days of experiment, the colon content was used for microbiota analyses; after isolation of total bacterial genomic DNA, V3/V4 regions of the 16S rRNA amplicons were sequenced using the Illumina MiSeq platform. The raw sequences were analyzed using the v.1.9.1 QIIME pipeline software. 157 numbers of OTUs were identified among all four dietary groups with 26 of them being prevalent above 0.05% in the total relative abundance. GS and AFB1 increase the relative abundance of phylum Bacteroidetes and Proteobacteria, while decreasing the Firmicutes abundance in a synergic manner as compared with the individual treatments. An additive or synergistic action of the two treatments was identified for Lactobacillus, Prevotella and Campylobacter, while rather an antagonistic effect was observed on Lachnospira. The action mechanisms of aflatoxin B1 and grape seed meal that drive the large intestine microbiota to these changes are not known and need further investigations. |
| first_indexed | 2024-04-13T00:42:03Z |
| format | Article |
| id | doaj.art-67e697b4d9fb4337a2dc843e257c7b68 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T00:42:03Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-67e697b4d9fb4337a2dc843e257c7b682022-12-22T03:10:07ZengMDPI AGToxins2072-66512019-01-011112510.3390/toxins11010025toxins11010025The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after WeaningIulian A. Grosu0Gina C. Pistol1Ionelia Taranu2Daniela E. Marin3Laboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti no. 1, Balotesti, Ilfov 077015, RomaniaLaboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti no. 1, Balotesti, Ilfov 077015, RomaniaLaboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti no. 1, Balotesti, Ilfov 077015, RomaniaLaboratory of Animal Biology, National Institute for Research and Development for Biology and Animal Nutrition, Calea Bucuresti no. 1, Balotesti, Ilfov 077015, RomaniaThe study investigated the effect of grape seed (GS) meal, aflatoxin (AFB1), or their combination on the large intestine microbiota of weanling piglets. Twenty-four piglets were allocated into four groups based on diet composition: (1) Control group; (2) AFB1 (320 g/kg feed) group; (3) GS group (8% inclusion in the diet); (4) AFB1 + GS group. After 30 days of experiment, the colon content was used for microbiota analyses; after isolation of total bacterial genomic DNA, V3/V4 regions of the 16S rRNA amplicons were sequenced using the Illumina MiSeq platform. The raw sequences were analyzed using the v.1.9.1 QIIME pipeline software. 157 numbers of OTUs were identified among all four dietary groups with 26 of them being prevalent above 0.05% in the total relative abundance. GS and AFB1 increase the relative abundance of phylum Bacteroidetes and Proteobacteria, while decreasing the Firmicutes abundance in a synergic manner as compared with the individual treatments. An additive or synergistic action of the two treatments was identified for Lactobacillus, Prevotella and Campylobacter, while rather an antagonistic effect was observed on Lachnospira. The action mechanisms of aflatoxin B1 and grape seed meal that drive the large intestine microbiota to these changes are not known and need further investigations.http://www.mdpi.com/2072-6651/11/1/25microbiotagrape seedaflatoxin B1 |
| spellingShingle | Iulian A. Grosu Gina C. Pistol Ionelia Taranu Daniela E. Marin The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning Toxins microbiota grape seed aflatoxin B1 |
| title | The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning |
| title_full | The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning |
| title_fullStr | The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning |
| title_full_unstemmed | The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning |
| title_short | The Impact of Dietary Grape Seed Meal on Healthy and Aflatoxin B1 Afflicted Microbiota of Pigs after Weaning |
| title_sort | impact of dietary grape seed meal on healthy and aflatoxin b1 afflicted microbiota of pigs after weaning |
| topic | microbiota grape seed aflatoxin B1 |
| url | http://www.mdpi.com/2072-6651/11/1/25 |
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