A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events
Integrin Adhesion Complexes (IACs) serve as links between the cytoskeleton and extracellular environment, acting as mechanosensing and signaling hubs. As such, IACs participate in many aspects of cellular motility, tissue morphogenesis, anchorage-dependent growth and cell survival. Focal Adhesion Ki...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023024891 |
| _version_ | 1797836952280498176 |
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| author | Jason A. Estep Lu O. Sun Martin M. Riccomagno |
| author_facet | Jason A. Estep Lu O. Sun Martin M. Riccomagno |
| author_sort | Jason A. Estep |
| collection | DOAJ |
| description | Integrin Adhesion Complexes (IACs) serve as links between the cytoskeleton and extracellular environment, acting as mechanosensing and signaling hubs. As such, IACs participate in many aspects of cellular motility, tissue morphogenesis, anchorage-dependent growth and cell survival. Focal Adhesion Kinase (FAK) has emerged as a critical organizer of IAC signaling events due to its early recruitment and diverse substrates, and thus has become a genetic and therapeutic target. Here we present the design and characterization of simple, reversible, and scalable Bimolecular Complementation sensors to monitor FAK phosphorylation in living cells. These probes provide novel means to quantify IAC signaling, expanding on the currently available toolkit for interrogating FAK phosphorylation during diverse cellular processes. |
| first_indexed | 2024-04-09T15:17:10Z |
| format | Article |
| id | doaj.art-67e765b9174a412781e16c5522f9d784 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T15:17:10Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-67e765b9174a412781e16c5522f9d7842023-04-29T14:56:03ZengElsevierHeliyon2405-84402023-04-0194e15282A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling eventsJason A. Estep0Lu O. Sun1Martin M. Riccomagno2Cell, Molecular and Developmental Biology Program, Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USADepartment of Molecular Biology, UT Southwestern Medical Center, Dallas, TX 75390, USACell, Molecular and Developmental Biology Program, Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA; Neuroscience Program, Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA; Corresponding author. Department of Molecular, Cell and Systems Biology, University of California, Riverside, CA 92521, USA.Integrin Adhesion Complexes (IACs) serve as links between the cytoskeleton and extracellular environment, acting as mechanosensing and signaling hubs. As such, IACs participate in many aspects of cellular motility, tissue morphogenesis, anchorage-dependent growth and cell survival. Focal Adhesion Kinase (FAK) has emerged as a critical organizer of IAC signaling events due to its early recruitment and diverse substrates, and thus has become a genetic and therapeutic target. Here we present the design and characterization of simple, reversible, and scalable Bimolecular Complementation sensors to monitor FAK phosphorylation in living cells. These probes provide novel means to quantify IAC signaling, expanding on the currently available toolkit for interrogating FAK phosphorylation during diverse cellular processes.http://www.sciencedirect.com/science/article/pii/S2405844023024891Integrin adhesion complexesFocal adhesionsFocal adhesion kinaseIntegrinCell migrationCell motility |
| spellingShingle | Jason A. Estep Lu O. Sun Martin M. Riccomagno A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events Heliyon Integrin adhesion complexes Focal adhesions Focal adhesion kinase Integrin Cell migration Cell motility |
| title | A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events |
| title_full | A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events |
| title_fullStr | A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events |
| title_full_unstemmed | A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events |
| title_short | A luciferase fragment complementation assay to detect focal adhesion kinase (FAK) signaling events |
| title_sort | luciferase fragment complementation assay to detect focal adhesion kinase fak signaling events |
| topic | Integrin adhesion complexes Focal adhesions Focal adhesion kinase Integrin Cell migration Cell motility |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023024891 |
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