Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea
<p>Abstract</p> <p>Background</p> <p>A Brucella eradication program has been executed in Korea. To effectively prevent and control brucellosis, a molecular method for genetic identification and epidemiological trace-back must be established. As part of that, the MLVA ty...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-10-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/9/230 |
| _version_ | 1818231296087818240 |
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| author | Jung Suk-Chan Heo Young-Ran Hwang In-Yeong Cho Yun-Sang Cho Dong-Hee Kang Sung-Il Her Moon Yoo Han-Sang |
| author_facet | Jung Suk-Chan Heo Young-Ran Hwang In-Yeong Cho Yun-Sang Cho Dong-Hee Kang Sung-Il Her Moon Yoo Han-Sang |
| author_sort | Jung Suk-Chan |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A Brucella eradication program has been executed in Korea. To effectively prevent and control brucellosis, a molecular method for genetic identification and epidemiological trace-back must be established. As part of that, the MLVA typing assay was evaluated and applied to <it>B. abortus </it>isolates for analyzing the characteristics of the regional distribution and relationships of foreign isolates.</p> <p>Results</p> <p>A total of 177 isolates originating from 105 cattle farms for the period 1996 to 2008 were selected as representatives for the nine provinces of South Korea. A dendrogram of strain relatedness was constructed in accordance with the number of tandem repeat units for 17 loci so that it was possible to trace back in the restricted areas. Even in a farm contaminated by one source, however, the <it>Brucella </it>isolates showed an increase or decrease in one TRs copy number at some loci with high DI values. Moreover, those 17 loci was confirmed in stability via <it>in-vitro </it>and <it>in-vivo </it>passage, and found to be sufficiently stable markers that can readily identify the inoculated strain even if minor changes were detected. In the parsimony analysis with foreign <it>Brucella </it>isolates, domestic isolates were clustered distinctively, and located near the Central and Southern American isolates.</p> <p>Conclusion</p> <p>The MLVA assay has enough discrimination power in the <it>Brucella </it>species level and can be utilized as a tool for the epidemiological trace-back of the <it>B. abortus </it>isolates. But it is important to consider that <it>Brucella </it>isolates may be capable of undergoing minor changes at some loci in the course of infection or in accordance with the changes of the host.</p> |
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| format | Article |
| id | doaj.art-67ec849a953d431ba0beeaf034d20e70 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-12T10:48:08Z |
| publishDate | 2009-10-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-67ec849a953d431ba0beeaf034d20e702022-12-22T00:26:50ZengBMCBMC Microbiology1471-21802009-10-019123010.1186/1471-2180-9-230Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in KoreaJung Suk-ChanHeo Young-RanHwang In-YeongCho Yun-SangCho Dong-HeeKang Sung-IlHer MoonYoo Han-Sang<p>Abstract</p> <p>Background</p> <p>A Brucella eradication program has been executed in Korea. To effectively prevent and control brucellosis, a molecular method for genetic identification and epidemiological trace-back must be established. As part of that, the MLVA typing assay was evaluated and applied to <it>B. abortus </it>isolates for analyzing the characteristics of the regional distribution and relationships of foreign isolates.</p> <p>Results</p> <p>A total of 177 isolates originating from 105 cattle farms for the period 1996 to 2008 were selected as representatives for the nine provinces of South Korea. A dendrogram of strain relatedness was constructed in accordance with the number of tandem repeat units for 17 loci so that it was possible to trace back in the restricted areas. Even in a farm contaminated by one source, however, the <it>Brucella </it>isolates showed an increase or decrease in one TRs copy number at some loci with high DI values. Moreover, those 17 loci was confirmed in stability via <it>in-vitro </it>and <it>in-vivo </it>passage, and found to be sufficiently stable markers that can readily identify the inoculated strain even if minor changes were detected. In the parsimony analysis with foreign <it>Brucella </it>isolates, domestic isolates were clustered distinctively, and located near the Central and Southern American isolates.</p> <p>Conclusion</p> <p>The MLVA assay has enough discrimination power in the <it>Brucella </it>species level and can be utilized as a tool for the epidemiological trace-back of the <it>B. abortus </it>isolates. But it is important to consider that <it>Brucella </it>isolates may be capable of undergoing minor changes at some loci in the course of infection or in accordance with the changes of the host.</p>http://www.biomedcentral.com/1471-2180/9/230 |
| spellingShingle | Jung Suk-Chan Heo Young-Ran Hwang In-Yeong Cho Yun-Sang Cho Dong-Hee Kang Sung-Il Her Moon Yoo Han-Sang Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea BMC Microbiology |
| title | Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea |
| title_full | Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea |
| title_fullStr | Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea |
| title_full_unstemmed | Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea |
| title_short | Application and evaluation of the MLVA typing assay for the <it>Brucella abortus </it>strains isolated in Korea |
| title_sort | application and evaluation of the mlva typing assay for the it brucella abortus it strains isolated in korea |
| url | http://www.biomedcentral.com/1471-2180/9/230 |
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