Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis
The present study was designed to evaluate the clinical outcome of cell-based therapy with cultured adipose-derived stromal cells (ASCs) for the treatment of cutaneous manifestations in patients affected by systemic sclerosis (SSc). ASCs have an extraordinary developmental plasticity, including the...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2013-05-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X639017 |
| _version_ | 1818256167547174912 |
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| author | Nicolò Scuderi Simona Ceccarelli Maria Giuseppina Onesti Paolo Fioramonti Chiara Guidi Ferdinando Romano Luigi Frati Antonio Angeloni Cinzia Marchese Ph.D. |
| author_facet | Nicolò Scuderi Simona Ceccarelli Maria Giuseppina Onesti Paolo Fioramonti Chiara Guidi Ferdinando Romano Luigi Frati Antonio Angeloni Cinzia Marchese Ph.D. |
| author_sort | Nicolò Scuderi |
| collection | DOAJ |
| description | The present study was designed to evaluate the clinical outcome of cell-based therapy with cultured adipose-derived stromal cells (ASCs) for the treatment of cutaneous manifestations in patients affected by systemic sclerosis (SSc). ASCs have an extraordinary developmental plasticity, including the ability to undergo multilineage differentiation and self-renewal. Moreover, ASCs can be easily harvested from small volumes of liposuction aspirate, showing great in vitro viability and proliferation rate. Here we isolated, characterized, and expanded ASCs, assessing both their mesenchymal origin and their capability to differentiate towards the adipogenic, osteogenic, and chondrogenic lineage. We developed an effective method for ASCs transplantation into sclerodermic patients by means of a hyaluronic acid (HA) solution, which allowed us to achieve precise structural modifications. ASCs were isolated from subcutaneous adipose tissue of six sclerodermic patients and cultured in a chemical-defined medium before autologous transplantation to restore skin sequelae. The results indicated that transplantation of a combination of ASCs in HA solution determined a significant improvement in tightening of the skin without complications such as anechoic areas, fat necrosis, or infections, thus suggesting that ASCs are a potentially valuable source of cells for skin therapy in rare diseases such as SSc and generally in skin disorders. |
| first_indexed | 2024-12-12T17:23:27Z |
| format | Article |
| id | doaj.art-67ef49734428481d94163bd8facc52f5 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T17:23:27Z |
| publishDate | 2013-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-67ef49734428481d94163bd8facc52f52022-12-22T00:17:35ZengSAGE PublishingCell Transplantation0963-68971555-38922013-05-012210.3727/096368912X639017Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic SclerosisNicolò Scuderi0Simona Ceccarelli1Maria Giuseppina Onesti2Paolo Fioramonti3Chiara Guidi4Ferdinando Romano5Luigi Frati6Antonio Angeloni7Cinzia Marchese Ph.D.8Department of Surgery “P. Valdoni,” University Sapienza, Rome, ItalyDepartment of Experimental Medicine, University Sapienza, Rome, ItalyDepartment of Surgery “P. Valdoni,” University Sapienza, Rome, ItalyDepartment of Surgery “P. Valdoni,” University Sapienza, Rome, ItalyBioscience Institute, Falciano, Republic of San MarinoDepartment of Public Health and Infectious Diseases, University Sapienza, Rome, ItalyDepartment of Molecular Medicine, University Sapienza, Rome, ItalyDepartment of Experimental Medicine, University Sapienza, Rome, ItalyDepartment of Experimental Medicine, University Sapienza, Rome, ItalyThe present study was designed to evaluate the clinical outcome of cell-based therapy with cultured adipose-derived stromal cells (ASCs) for the treatment of cutaneous manifestations in patients affected by systemic sclerosis (SSc). ASCs have an extraordinary developmental plasticity, including the ability to undergo multilineage differentiation and self-renewal. Moreover, ASCs can be easily harvested from small volumes of liposuction aspirate, showing great in vitro viability and proliferation rate. Here we isolated, characterized, and expanded ASCs, assessing both their mesenchymal origin and their capability to differentiate towards the adipogenic, osteogenic, and chondrogenic lineage. We developed an effective method for ASCs transplantation into sclerodermic patients by means of a hyaluronic acid (HA) solution, which allowed us to achieve precise structural modifications. ASCs were isolated from subcutaneous adipose tissue of six sclerodermic patients and cultured in a chemical-defined medium before autologous transplantation to restore skin sequelae. The results indicated that transplantation of a combination of ASCs in HA solution determined a significant improvement in tightening of the skin without complications such as anechoic areas, fat necrosis, or infections, thus suggesting that ASCs are a potentially valuable source of cells for skin therapy in rare diseases such as SSc and generally in skin disorders.https://doi.org/10.3727/096368912X639017 |
| spellingShingle | Nicolò Scuderi Simona Ceccarelli Maria Giuseppina Onesti Paolo Fioramonti Chiara Guidi Ferdinando Romano Luigi Frati Antonio Angeloni Cinzia Marchese Ph.D. Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis Cell Transplantation |
| title | Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis |
| title_full | Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis |
| title_fullStr | Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis |
| title_full_unstemmed | Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis |
| title_short | Human Adipose-Derived Stromal Cells for Cell-Based Therapies in the Treatment of Systemic Sclerosis |
| title_sort | human adipose derived stromal cells for cell based therapies in the treatment of systemic sclerosis |
| url | https://doi.org/10.3727/096368912X639017 |
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