3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo
Calcium phosphate bio-ceramics are osteo-conductive, but it remains a challenge to promote the induction of bone augmentation and capillary formation. The surface micro/nano-topography of materials can be recognized by cells and then the cell fate are mediated. Traditional regulation methods of carv...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2022-06-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21004801 |
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| author | Xiao Liu Yali Miao Haifeng Liang Jingjing Diao Lijing Hao Zhifeng Shi Naru Zhao Yingjun Wang |
| author_facet | Xiao Liu Yali Miao Haifeng Liang Jingjing Diao Lijing Hao Zhifeng Shi Naru Zhao Yingjun Wang |
| author_sort | Xiao Liu |
| collection | DOAJ |
| description | Calcium phosphate bio-ceramics are osteo-conductive, but it remains a challenge to promote the induction of bone augmentation and capillary formation. The surface micro/nano-topography of materials can be recognized by cells and then the cell fate are mediated. Traditional regulation methods of carving surface structures on bio-ceramics employ mineral reagents and organic additives, which might introduce impurity phases and affect the biological results. In a previous study, a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment, and a uniform hydroxyapatite (HAp) surface layer was constructed on composite ceramics (β-TCP/CaSiO3) in situ. Further combined with 3D printing technology, biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface layer. The obtained HAp surface layer favoured cell adhesion, alleviated the cytotoxicity of precursor scaffolds, and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in vitro. In vivo studies showed that capillary formation, bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained, and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration. |
| first_indexed | 2024-04-24T08:43:12Z |
| format | Article |
| id | doaj.art-67f0692743ea46598f93cec6df4c0313 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:43:12Z |
| publishDate | 2022-06-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Bioactive Materials |
| spelling | doaj.art-67f0692743ea46598f93cec6df4c03132024-04-16T14:44:14ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2022-06-01121201323D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivoXiao Liu0Yali Miao1Haifeng Liang2Jingjing Diao3Lijing Hao4Zhifeng Shi5Naru Zhao6Yingjun Wang7National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, ChinaSchool of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR ChinaDepartment of Orthopedics, Zhujiang Hospital, Southern Medical University, No. 253 Industrial Avenue, Haizhu, Guangzhou, 510280, People's Republic of ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Medical Devices Research & Testing Center of SCUT, Guangzhou, 510006, PR ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Medical Devices Research & Testing Center of SCUT, Guangzhou, 510006, PR ChinaNational Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China; Corresponding author. School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.;National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China; School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Guangdong Province Key Laboratory of Biomedical Engineering, South China University of Technology, Guangzhou, 510006, China; Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education, South China University of Technology, Guangzhou, 510006, PR China; Innovation Center for Tissue Restoration and Reconstruction, South China University of Technology, Guangzhou, 510006, PR China; Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory), 510005 Guangzhou, China; Corresponding author. National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou, China.Calcium phosphate bio-ceramics are osteo-conductive, but it remains a challenge to promote the induction of bone augmentation and capillary formation. The surface micro/nano-topography of materials can be recognized by cells and then the cell fate are mediated. Traditional regulation methods of carving surface structures on bio-ceramics employ mineral reagents and organic additives, which might introduce impurity phases and affect the biological results. In a previous study, a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment, and a uniform hydroxyapatite (HAp) surface layer was constructed on composite ceramics (β-TCP/CaSiO3) in situ. Further combined with 3D printing technology, biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface layer. The obtained HAp surface layer favoured cell adhesion, alleviated the cytotoxicity of precursor scaffolds, and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in vitro. In vivo studies showed that capillary formation, bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained, and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration.http://www.sciencedirect.com/science/article/pii/S2452199X210048013D printed porous scaffoldBioactive ceramicsHAp surface layerCell fate mediationBone augmentation |
| spellingShingle | Xiao Liu Yali Miao Haifeng Liang Jingjing Diao Lijing Hao Zhifeng Shi Naru Zhao Yingjun Wang 3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo Bioactive Materials 3D printed porous scaffold Bioactive ceramics HAp surface layer Cell fate mediation Bone augmentation |
| title | 3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo |
| title_full | 3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo |
| title_fullStr | 3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo |
| title_full_unstemmed | 3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo |
| title_short | 3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone–implant interface in vivo |
| title_sort | 3d printed bioactive ceramic scaffolds with biomimetic micro nano hap surfaces mediated cell fate and promoted bone augmentation of the bone implant interface in vivo |
| topic | 3D printed porous scaffold Bioactive ceramics HAp surface layer Cell fate mediation Bone augmentation |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21004801 |
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