Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae
Abstract Habitat loss, the introduction of invasive species, and climate change due to human activity have threatened many freshwater unionid bivalves worldwide. Unionid bivalves represent important members of freshwater ecosystems, providing bitterling fish with spawning grounds and contributing to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Environmental DNA |
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| Online Access: | https://doi.org/10.1002/edn3.304 |
| _version_ | 1818472217187450880 |
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| author | Kotaro Sugawara Yudai Sasaki Kunihiro Okano Miho Watanabe Naoyuki Miyata |
| author_facet | Kotaro Sugawara Yudai Sasaki Kunihiro Okano Miho Watanabe Naoyuki Miyata |
| author_sort | Kotaro Sugawara |
| collection | DOAJ |
| description | Abstract Habitat loss, the introduction of invasive species, and climate change due to human activity have threatened many freshwater unionid bivalves worldwide. Unionid bivalves represent important members of freshwater ecosystems, providing bitterling fish with spawning grounds and contributing to water clarification via the filtration of suspended solids. This study examined an environmental DNA (eDNA) approach for monitoring the unionid bivalve Nodularia nipponensis in a lake environment. We developed oligonucleotide primers for the specific detection of mitochondrial DNA from N. nipponensis and evaluated the decay and release rates of eDNA in a laboratory tank experiment under two bivalve densities (49 and 164 individuals m−2) selected to ensure sufficient eDNA yields. The eDNA release rate was weakly and positively correlated with bivalve density but strongly and positively correlated with inorganic nitrogen and phosphorus excretion from the bivalve. In a eutrophic lake, Lake Hachiro, 67% of the samples (16 out of 24) collected from June to July yielded detectable amounts of eDNA and were not detected at all in May and August (0 out of 20). This difference was probably due to the release of N. nipponensis glochidium larvae. During these months, unionid glochidia adhered to bundles of polyvinylidene chloride fibers placed on the lake bottom for 8 h, confirmed by microscopic inspection. We successfully obtained N. nipponensis eDNA from all bundles examined over the months, demonstrating the efficacy of bundle capture in quantifying unionid bivalve eDNA. Our eDNA‐based technique with specific oligonucleotide primers and fiber bundles may be a promising tool for monitoring N. nipponensis in freshwater environments. Fiber bundles are useful for tracing eDNA derived from glochidium larvae, even at low unionid bivalve densities, which may be conducive to conservation efforts. |
| first_indexed | 2024-04-14T04:03:41Z |
| format | Article |
| id | doaj.art-67f1ac3b174e4bafa81b69d28f67090a |
| institution | Directory Open Access Journal |
| issn | 2637-4943 |
| language | English |
| last_indexed | 2024-04-14T04:03:41Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Environmental DNA |
| spelling | doaj.art-67f1ac3b174e4bafa81b69d28f67090a2022-12-22T02:13:26ZengWileyEnvironmental DNA2637-49432022-07-014490891910.1002/edn3.304Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvaeKotaro Sugawara0Yudai Sasaki1Kunihiro Okano2Miho Watanabe3Naoyuki Miyata4Graduate School of Bioresource Sciences Akita Prefectural University Akita JapanDepartment of Biological Environment Akita Prefectural University Akita JapanDepartment of Biological Environment Akita Prefectural University Akita JapanDepartment of Biological Environment Akita Prefectural University Akita JapanDepartment of Biological Environment Akita Prefectural University Akita JapanAbstract Habitat loss, the introduction of invasive species, and climate change due to human activity have threatened many freshwater unionid bivalves worldwide. Unionid bivalves represent important members of freshwater ecosystems, providing bitterling fish with spawning grounds and contributing to water clarification via the filtration of suspended solids. This study examined an environmental DNA (eDNA) approach for monitoring the unionid bivalve Nodularia nipponensis in a lake environment. We developed oligonucleotide primers for the specific detection of mitochondrial DNA from N. nipponensis and evaluated the decay and release rates of eDNA in a laboratory tank experiment under two bivalve densities (49 and 164 individuals m−2) selected to ensure sufficient eDNA yields. The eDNA release rate was weakly and positively correlated with bivalve density but strongly and positively correlated with inorganic nitrogen and phosphorus excretion from the bivalve. In a eutrophic lake, Lake Hachiro, 67% of the samples (16 out of 24) collected from June to July yielded detectable amounts of eDNA and were not detected at all in May and August (0 out of 20). This difference was probably due to the release of N. nipponensis glochidium larvae. During these months, unionid glochidia adhered to bundles of polyvinylidene chloride fibers placed on the lake bottom for 8 h, confirmed by microscopic inspection. We successfully obtained N. nipponensis eDNA from all bundles examined over the months, demonstrating the efficacy of bundle capture in quantifying unionid bivalve eDNA. Our eDNA‐based technique with specific oligonucleotide primers and fiber bundles may be a promising tool for monitoring N. nipponensis in freshwater environments. Fiber bundles are useful for tracing eDNA derived from glochidium larvae, even at low unionid bivalve densities, which may be conducive to conservation efforts.https://doi.org/10.1002/edn3.304bivalveconservationenvironmental DNAmusselUnionidae |
| spellingShingle | Kotaro Sugawara Yudai Sasaki Kunihiro Okano Miho Watanabe Naoyuki Miyata Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae Environmental DNA bivalve conservation environmental DNA mussel Unionidae |
| title | Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae |
| title_full | Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae |
| title_fullStr | Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae |
| title_full_unstemmed | Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae |
| title_short | Application of eDNA for monitoring freshwater bivalve Nodularia nipponensis and its glochidium larvae |
| title_sort | application of edna for monitoring freshwater bivalve nodularia nipponensis and its glochidium larvae |
| topic | bivalve conservation environmental DNA mussel Unionidae |
| url | https://doi.org/10.1002/edn3.304 |
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