T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster
Wei Chen,1,* Lu Zhu,1,* Li-Ling Shen,1 Shao-Yan Si,2 Jun-Lian Liu3 1Dermatology, Zhejiang Provincial Dermatology Hospital, Huzhou, Zhejiang, People’s Republic of China; 2Department of Comprehensive Basic Experiment, Strategic Support Force Medical Center, Bejing, People’s Republic of...
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Dove Medical Press
2023-05-01
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| Series: | Journal of Pain Research |
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| Online Access: | https://www.dovepress.com/t-lymphocyte-subsets-profile-and-toll-like-receptors-responses-in-pati-peer-reviewed-fulltext-article-JPR |
| _version_ | 1827944414697226240 |
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| author | Chen W Zhu L Shen LL Si SY Liu JL |
| author_facet | Chen W Zhu L Shen LL Si SY Liu JL |
| author_sort | Chen W |
| collection | DOAJ |
| description | Wei Chen,1,* Lu Zhu,1,* Li-Ling Shen,1 Shao-Yan Si,2 Jun-Lian Liu3 1Dermatology, Zhejiang Provincial Dermatology Hospital, Huzhou, Zhejiang, People’s Republic of China; 2Department of Comprehensive Basic Experiment, Strategic Support Force Medical Center, Bejing, People’s Republic of China; 3Dermatology, Chui Yang Liu Hospital Affiliated Tsinghua University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun-Lian Liu, Chui Yang Liu Hospital affiliated Tsinghua University, 2 Chuiyangliu South Street, Chaoyang District, Beijing, 100022, People’s Republic of China, Tel +86-13801214526, Email liujunlian306@sina.comPurpose: Herpes zoster (HZ) is caused by the varicella-zoster virus (VZV), and 20% of healthy humans and 50% of people with immune dysfunction have a high probability of suffering from HZ. This study aimed to screen dynamic immune signatures and explore the potential mechanism during HZ progression.Patients and Methods: Peripheral blood samples from 31 HZ patients and 32 age-sex-matched healthy controls were collected and analyzed. The protein levels and gene levels of toll-like receptors (TLRs) were detected in peripheral blood mononuclear cells (PBMCs) by flow cytometry and quantitative real-time PCR. Further, the characteristics of T cell subsets and cytokines were detected via a cytometric bead array.Results: Compared to healthy controls, the mRNA levels of TLR2, TLR4, TLR7, and TLR9 mRNA in PBMCs were significantly increased in HZ patients. The protein level of TLR4 and TLR7 was significantly increased in HZ patients, but the levels of TLR2 and TLR9 were dramatically decreased. The CD3+ T cells were constant in HZ and healthy controls. CD4+ T cells were decreased in HZ patients, while CD8+ T cells were increased, resulting in an improved CD4+/CD8+ T cells ratio. Further, it was found that Th2 and Th17 were not changed, but the decreased Th1 and upregulated Treg cells were found in HZ. The Th1/Th2 and Th17/Treg ratios were significantly decreased. Last, the levels of IL-6, IL-10, and IFN-γ were significantly increased, but IL-2, IL-4, and IL-17A had no significant changes.Conclusion: The dysfunction of host’s lymphocytes and activation of TLRs in PBMCs were the important mechanism in varicella-zoster virus induced herpes zoster. TLRs might be the core targets for the therapy drug development in treating HZ.Keywords: herpes zoster, peripheral blood mononuclear cells, T lymphocyte, toll-like receptors |
| first_indexed | 2024-03-13T10:29:58Z |
| format | Article |
| id | doaj.art-67f2dcb4d7854c2189613c7cded5bad0 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-03-13T10:29:58Z |
| publishDate | 2023-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Pain Research |
| spelling | doaj.art-67f2dcb4d7854c2189613c7cded5bad02023-05-18T18:42:34ZengDove Medical PressJournal of Pain Research1178-70902023-05-01Volume 161581159483798T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes ZosterChen WZhu LShen LLSi SYLiu JLWei Chen,1,* Lu Zhu,1,* Li-Ling Shen,1 Shao-Yan Si,2 Jun-Lian Liu3 1Dermatology, Zhejiang Provincial Dermatology Hospital, Huzhou, Zhejiang, People’s Republic of China; 2Department of Comprehensive Basic Experiment, Strategic Support Force Medical Center, Bejing, People’s Republic of China; 3Dermatology, Chui Yang Liu Hospital Affiliated Tsinghua University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jun-Lian Liu, Chui Yang Liu Hospital affiliated Tsinghua University, 2 Chuiyangliu South Street, Chaoyang District, Beijing, 100022, People’s Republic of China, Tel +86-13801214526, Email liujunlian306@sina.comPurpose: Herpes zoster (HZ) is caused by the varicella-zoster virus (VZV), and 20% of healthy humans and 50% of people with immune dysfunction have a high probability of suffering from HZ. This study aimed to screen dynamic immune signatures and explore the potential mechanism during HZ progression.Patients and Methods: Peripheral blood samples from 31 HZ patients and 32 age-sex-matched healthy controls were collected and analyzed. The protein levels and gene levels of toll-like receptors (TLRs) were detected in peripheral blood mononuclear cells (PBMCs) by flow cytometry and quantitative real-time PCR. Further, the characteristics of T cell subsets and cytokines were detected via a cytometric bead array.Results: Compared to healthy controls, the mRNA levels of TLR2, TLR4, TLR7, and TLR9 mRNA in PBMCs were significantly increased in HZ patients. The protein level of TLR4 and TLR7 was significantly increased in HZ patients, but the levels of TLR2 and TLR9 were dramatically decreased. The CD3+ T cells were constant in HZ and healthy controls. CD4+ T cells were decreased in HZ patients, while CD8+ T cells were increased, resulting in an improved CD4+/CD8+ T cells ratio. Further, it was found that Th2 and Th17 were not changed, but the decreased Th1 and upregulated Treg cells were found in HZ. The Th1/Th2 and Th17/Treg ratios were significantly decreased. Last, the levels of IL-6, IL-10, and IFN-γ were significantly increased, but IL-2, IL-4, and IL-17A had no significant changes.Conclusion: The dysfunction of host’s lymphocytes and activation of TLRs in PBMCs were the important mechanism in varicella-zoster virus induced herpes zoster. TLRs might be the core targets for the therapy drug development in treating HZ.Keywords: herpes zoster, peripheral blood mononuclear cells, T lymphocyte, toll-like receptorshttps://www.dovepress.com/t-lymphocyte-subsets-profile-and-toll-like-receptors-responses-in-pati-peer-reviewed-fulltext-article-JPRherpes zosterperipheral blood mononuclear cellst lymphocytetoll-like receptors. |
| spellingShingle | Chen W Zhu L Shen LL Si SY Liu JL T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster Journal of Pain Research herpes zoster peripheral blood mononuclear cells t lymphocyte toll-like receptors. |
| title | T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster |
| title_full | T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster |
| title_fullStr | T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster |
| title_full_unstemmed | T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster |
| title_short | T Lymphocyte Subsets Profile and Toll-Like Receptors Responses in Patients with Herpes Zoster |
| title_sort | t lymphocyte subsets profile and toll like receptors responses in patients with herpes zoster |
| topic | herpes zoster peripheral blood mononuclear cells t lymphocyte toll-like receptors. |
| url | https://www.dovepress.com/t-lymphocyte-subsets-profile-and-toll-like-receptors-responses-in-pati-peer-reviewed-fulltext-article-JPR |
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