Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri
Abstract Background Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence. The goal of this study was to characterize the biological function of hfq gene in Xanthomonas axonpodis pv. citri (X...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1476-9 |
| _version_ | 1819057250229026816 |
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| author | Xuelu Liu Yuping Yan Haodi Wu Changyong Zhou Xuefeng Wang |
| author_facet | Xuelu Liu Yuping Yan Haodi Wu Changyong Zhou Xuefeng Wang |
| author_sort | Xuelu Liu |
| collection | DOAJ |
| description | Abstract Background Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence. The goal of this study was to characterize the biological function of hfq gene in Xanthomonas axonpodis pv. citri (Xac), the causal agent of citrus canker disease. Results An hfq mutant in Xac was generated by plasmid integration. The loss of hfq resulted in attenuation of bacterial growth, motility and biofilm formation. In addition, the hfq mutation impaired Xac resistance to H2O2 and both high and low pH environments, but did not affect the virulence to citrus. RNA-Seq analyses indicated that Hfq played roles in regulating the expression of 746 genes. In hfq mutant, gene expression related to chemotaxis, secretion system, two-component system, quorum sensing and flagellar assembly were repressed, whereas expression of ribosomal genes were significantly up-regulated. The down-regulated expression of three bacterial chemotaxis related genes and seven flagella genes, which involved in cell growth and biofilm formation, were further validated by RT-qPCR. Conclusions The study demonstrated that hfq was involved in multiple biological processes in Xac. The results could serve as initiate points for identifying regulatory sRNAs and genes controlled by Hfq-sRNA interactions in Xac. |
| first_indexed | 2024-12-21T13:36:19Z |
| format | Article |
| id | doaj.art-67f485ccaf3f43f29ca4c8afe9db6271 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-21T13:36:19Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-67f485ccaf3f43f29ca4c8afe9db62712022-12-21T19:02:09ZengBMCBMC Microbiology1471-21802019-05-011911910.1186/s12866-019-1476-9Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citriXuelu Liu0Yuping Yan1Haodi Wu2Changyong Zhou3Xuefeng Wang4National Engineering Research Center for Citrus, Citrus Research Institute, Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for Citrus, Citrus Research Institute, Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for Citrus, Citrus Research Institute, Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for Citrus, Citrus Research Institute, Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for Citrus, Citrus Research Institute, Southwest University/Chinese Academy of Agricultural SciencesAbstract Background Hfq is a widely conserved bacterial RNA-binding protein which generally mediates the global regulatory activities involv ed in physiological process and virulence. The goal of this study was to characterize the biological function of hfq gene in Xanthomonas axonpodis pv. citri (Xac), the causal agent of citrus canker disease. Results An hfq mutant in Xac was generated by plasmid integration. The loss of hfq resulted in attenuation of bacterial growth, motility and biofilm formation. In addition, the hfq mutation impaired Xac resistance to H2O2 and both high and low pH environments, but did not affect the virulence to citrus. RNA-Seq analyses indicated that Hfq played roles in regulating the expression of 746 genes. In hfq mutant, gene expression related to chemotaxis, secretion system, two-component system, quorum sensing and flagellar assembly were repressed, whereas expression of ribosomal genes were significantly up-regulated. The down-regulated expression of three bacterial chemotaxis related genes and seven flagella genes, which involved in cell growth and biofilm formation, were further validated by RT-qPCR. Conclusions The study demonstrated that hfq was involved in multiple biological processes in Xac. The results could serve as initiate points for identifying regulatory sRNAs and genes controlled by Hfq-sRNA interactions in Xac.http://link.springer.com/article/10.1186/s12866-019-1476-9Xanthomonas axonpodis pv. citriXanthomonas citri subsp. citrihfq geneBiofilm |
| spellingShingle | Xuelu Liu Yuping Yan Haodi Wu Changyong Zhou Xuefeng Wang Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri BMC Microbiology Xanthomonas axonpodis pv. citri Xanthomonas citri subsp. citri hfq gene Biofilm |
| title | Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri |
| title_full | Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri |
| title_fullStr | Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri |
| title_full_unstemmed | Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri |
| title_short | Biological and transcriptomic studies reveal hfq is required for swimming, biofilm formation and stress response in Xanthomonas axonpodis pv. citri |
| title_sort | biological and transcriptomic studies reveal hfq is required for swimming biofilm formation and stress response in xanthomonas axonpodis pv citri |
| topic | Xanthomonas axonpodis pv. citri Xanthomonas citri subsp. citri hfq gene Biofilm |
| url | http://link.springer.com/article/10.1186/s12866-019-1476-9 |
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