Transgene-induced gene silencing is not affected by a change in ploidy level.
BACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants. Polyploidy can result in changes to gene expression and epigenetic instability. Several epigenetic phenomena, occurring at the trans...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2516530?pdf=render |
| _version_ | 1818363389076832256 |
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| author | Daniela Pignatta Brian Dilkes Tadeusz Wroblewski Richard W Michelmore Luca Comai |
| author_facet | Daniela Pignatta Brian Dilkes Tadeusz Wroblewski Richard W Michelmore Luca Comai |
| author_sort | Daniela Pignatta |
| collection | DOAJ |
| description | BACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants. Polyploidy can result in changes to gene expression and epigenetic instability. Several epigenetic phenomena, occurring at the transcriptional or post-transcriptional level, have been documented in allopolyploids (polyploids derived from species hybrids) of Arabidopsis thaliana, yet findings in autopolyploids (polyploids derived from the duplication of the genome of a single species) are limited. Here, we tested the hypothesis that an increase in ploidy enhances transgene-induced post-transcriptional gene silencing using autopolyploids of A. thaliana. METHODOLOGY/PRINCIPAL FINDINGS: Diploid and tetraploid individuals of four independent homozygous transgenic lines of A. thaliana transformed with chalcone synthase (CHS) inverted repeat (hairpin) constructs were generated. For each line diploids and tetraploids were compared for efficiency in post-transcriptional silencing of the endogenous CHS gene. The four lines differed substantially in their silencing efficiency. Yet, diploid and tetraploid plants derived from these plants and containing therefore identical transgene insertions showed no difference in the efficiency silencing CHS as assayed by visual scoring, anthocyanin assays and quantification of CHS mRNA. CONCLUSIONS/SIGNIFICANCE: Our results in A. thaliana indicated that there is no effect of ploidy level on transgene-induced post-transcriptional gene silencing. Our findings that post-transcriptional mechanisms were equally effective in diploids and tetraploids supports the use of transgene-driven post-transcriptional gene silencing as a useful mechanism to modify gene expression in polyploid species. |
| first_indexed | 2024-12-13T21:47:42Z |
| format | Article |
| id | doaj.art-67f6f4b19f6a4e63a2aa662cf0d3be57 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T21:47:42Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-67f6f4b19f6a4e63a2aa662cf0d3be572022-12-21T23:30:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0138e306110.1371/journal.pone.0003061Transgene-induced gene silencing is not affected by a change in ploidy level.Daniela PignattaBrian DilkesTadeusz WroblewskiRichard W MichelmoreLuca ComaiBACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants. Polyploidy can result in changes to gene expression and epigenetic instability. Several epigenetic phenomena, occurring at the transcriptional or post-transcriptional level, have been documented in allopolyploids (polyploids derived from species hybrids) of Arabidopsis thaliana, yet findings in autopolyploids (polyploids derived from the duplication of the genome of a single species) are limited. Here, we tested the hypothesis that an increase in ploidy enhances transgene-induced post-transcriptional gene silencing using autopolyploids of A. thaliana. METHODOLOGY/PRINCIPAL FINDINGS: Diploid and tetraploid individuals of four independent homozygous transgenic lines of A. thaliana transformed with chalcone synthase (CHS) inverted repeat (hairpin) constructs were generated. For each line diploids and tetraploids were compared for efficiency in post-transcriptional silencing of the endogenous CHS gene. The four lines differed substantially in their silencing efficiency. Yet, diploid and tetraploid plants derived from these plants and containing therefore identical transgene insertions showed no difference in the efficiency silencing CHS as assayed by visual scoring, anthocyanin assays and quantification of CHS mRNA. CONCLUSIONS/SIGNIFICANCE: Our results in A. thaliana indicated that there is no effect of ploidy level on transgene-induced post-transcriptional gene silencing. Our findings that post-transcriptional mechanisms were equally effective in diploids and tetraploids supports the use of transgene-driven post-transcriptional gene silencing as a useful mechanism to modify gene expression in polyploid species.http://europepmc.org/articles/PMC2516530?pdf=render |
| spellingShingle | Daniela Pignatta Brian Dilkes Tadeusz Wroblewski Richard W Michelmore Luca Comai Transgene-induced gene silencing is not affected by a change in ploidy level. PLoS ONE |
| title | Transgene-induced gene silencing is not affected by a change in ploidy level. |
| title_full | Transgene-induced gene silencing is not affected by a change in ploidy level. |
| title_fullStr | Transgene-induced gene silencing is not affected by a change in ploidy level. |
| title_full_unstemmed | Transgene-induced gene silencing is not affected by a change in ploidy level. |
| title_short | Transgene-induced gene silencing is not affected by a change in ploidy level. |
| title_sort | transgene induced gene silencing is not affected by a change in ploidy level |
| url | http://europepmc.org/articles/PMC2516530?pdf=render |
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