A quality control of proteomic experiments based on multiple isotopologous internal standards
The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | EuPA Open Proteomics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212968515300131 |
| _version_ | 1819098778766934016 |
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| author | Adele Bourmaud Sebastien Gallien Bruno Domon |
| author_facet | Adele Bourmaud Sebastien Gallien Bruno Domon |
| author_sort | Adele Bourmaud |
| collection | DOAJ |
| description | The harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications. |
| first_indexed | 2024-12-22T00:36:24Z |
| format | Article |
| id | doaj.art-67f8cf59464841e0bf5c0662e6cee21a |
| institution | Directory Open Access Journal |
| issn | 2212-9685 |
| language | English |
| last_indexed | 2024-12-22T00:36:24Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EuPA Open Proteomics |
| spelling | doaj.art-67f8cf59464841e0bf5c0662e6cee21a2022-12-21T18:44:47ZengElsevierEuPA Open Proteomics2212-96852015-09-018C162110.1016/j.euprot.2015.07.010A quality control of proteomic experiments based on multiple isotopologous internal standardsAdele Bourmaud0Sebastien Gallien1Bruno Domon2Luxembourg Clinical Proteomics Center (LCP), Luxembourg Institute of Health (LIH), Strassen, LuxembourgLuxembourg Clinical Proteomics Center (LCP), Luxembourg Institute of Health (LIH), Strassen, LuxembourgLuxembourg Clinical Proteomics Center (LCP), Luxembourg Institute of Health (LIH), Strassen, LuxembourgThe harmonization of proteomics experiments facilitates the exchange and comparison of results. The definition of standards and metrics ensures reliable and consistent data quality. An internal quality control procedure was developed to assess the different steps of a proteomic analysis workflow and perform a system suitability test. The method relies on a straightforward protocol using a simple mixture of exogenous proteins, and the sequential addition of two sets of isotopically labeled peptides added to reference samples. This internal quality control procedure was applied to plasma samples to demonstrate its easy implementation, which makes it generic for most proteomics applications.http://www.sciencedirect.com/science/article/pii/S2212968515300131Quality controlInternal standardPRMSRMIsotopically labeled peptides |
| spellingShingle | Adele Bourmaud Sebastien Gallien Bruno Domon A quality control of proteomic experiments based on multiple isotopologous internal standards EuPA Open Proteomics Quality control Internal standard PRM SRM Isotopically labeled peptides |
| title | A quality control of proteomic experiments based on multiple isotopologous internal standards |
| title_full | A quality control of proteomic experiments based on multiple isotopologous internal standards |
| title_fullStr | A quality control of proteomic experiments based on multiple isotopologous internal standards |
| title_full_unstemmed | A quality control of proteomic experiments based on multiple isotopologous internal standards |
| title_short | A quality control of proteomic experiments based on multiple isotopologous internal standards |
| title_sort | quality control of proteomic experiments based on multiple isotopologous internal standards |
| topic | Quality control Internal standard PRM SRM Isotopically labeled peptides |
| url | http://www.sciencedirect.com/science/article/pii/S2212968515300131 |
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