Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs.
The Rab11-FIPs (Rab11-family interacting proteins; henceforth, FIPs) are a family of Rab11a/Rab11b/Rab25 GTPase effector proteins implicated in an assortment of intracellular trafficking processes. Through proteomic screening, we have identified TSG101 (tumor susceptibility gene 101), a component of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3279423?pdf=render |
| _version_ | 1818955504546742272 |
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| author | Conor P Horgan Sara R Hanscom Eoin E Kelly Mary W McCaffrey |
| author_facet | Conor P Horgan Sara R Hanscom Eoin E Kelly Mary W McCaffrey |
| author_sort | Conor P Horgan |
| collection | DOAJ |
| description | The Rab11-FIPs (Rab11-family interacting proteins; henceforth, FIPs) are a family of Rab11a/Rab11b/Rab25 GTPase effector proteins implicated in an assortment of intracellular trafficking processes. Through proteomic screening, we have identified TSG101 (tumor susceptibility gene 101), a component of the ESCRT-I (endosomal sorting complex required for transport) complex, as a novel FIP4-binding protein, which we find can also bind FIP3. We show that α-helical coiled-coil regions of both TSG101 and FIP4 mediate the interaction with the cognate protein, and that point mutations in the coiled-coil regions of both TSG101 and FIP4 abrogate the interaction. We find that expression of TSG101 and FIP4 mutants cause cytokinesis defects, but that the TSG101-FIP4 interaction is not required for localisation of TSG101 to the midbody/Flemming body during abscission. Together, these data suggest functional overlap between Rab11-controlled processes and components of the ESCRT pathway. |
| first_indexed | 2024-12-20T10:39:07Z |
| format | Article |
| id | doaj.art-67ffd9ef71bd4c9a9642eb7b8fe355a6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T10:39:07Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-67ffd9ef71bd4c9a9642eb7b8fe355a62022-12-21T19:43:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0172e3203010.1371/journal.pone.0032030Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs.Conor P HorganSara R HanscomEoin E KellyMary W McCaffreyThe Rab11-FIPs (Rab11-family interacting proteins; henceforth, FIPs) are a family of Rab11a/Rab11b/Rab25 GTPase effector proteins implicated in an assortment of intracellular trafficking processes. Through proteomic screening, we have identified TSG101 (tumor susceptibility gene 101), a component of the ESCRT-I (endosomal sorting complex required for transport) complex, as a novel FIP4-binding protein, which we find can also bind FIP3. We show that α-helical coiled-coil regions of both TSG101 and FIP4 mediate the interaction with the cognate protein, and that point mutations in the coiled-coil regions of both TSG101 and FIP4 abrogate the interaction. We find that expression of TSG101 and FIP4 mutants cause cytokinesis defects, but that the TSG101-FIP4 interaction is not required for localisation of TSG101 to the midbody/Flemming body during abscission. Together, these data suggest functional overlap between Rab11-controlled processes and components of the ESCRT pathway.http://europepmc.org/articles/PMC3279423?pdf=render |
| spellingShingle | Conor P Horgan Sara R Hanscom Eoin E Kelly Mary W McCaffrey Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. PLoS ONE |
| title | Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. |
| title_full | Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. |
| title_fullStr | Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. |
| title_full_unstemmed | Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. |
| title_short | Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs. |
| title_sort | tumor susceptibility gene 101 tsg101 is a novel binding partner for the class ii rab11 fips |
| url | http://europepmc.org/articles/PMC3279423?pdf=render |
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