A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus
Abstract To prevent the generation of drug‐resistant fungi from long‐term exposure to microorganisms, cinnamic acid (CA), a natural effective antifungal agent, was successfully coupled with poly‐dopamine magnetic nanoparticles (CA–DMPs). Due to the low solubility of CA, the saturated solution of CA...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-06-01
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| Series: | Food Frontiers |
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| Online Access: | https://doi.org/10.1002/fft2.225 |
| _version_ | 1797808954465583104 |
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| author | Zihan Song Yanli Zhang Yongrui Ti Huitian Qiao Chen Niu Yuqian Ban Xiaoxiao Wang Yuqing Hou Ruiwen Lu Zihan Song |
| author_facet | Zihan Song Yanli Zhang Yongrui Ti Huitian Qiao Chen Niu Yuqian Ban Xiaoxiao Wang Yuqing Hou Ruiwen Lu Zihan Song |
| author_sort | Zihan Song |
| collection | DOAJ |
| description | Abstract To prevent the generation of drug‐resistant fungi from long‐term exposure to microorganisms, cinnamic acid (CA), a natural effective antifungal agent, was successfully coupled with poly‐dopamine magnetic nanoparticles (CA–DMPs). Due to the low solubility of CA, the saturated solution of CA (1.61 mg/mL, 45°C) had no antifungal effect. Contrarily, CA–DMPs showed a good antifungal effect. The resulting heat‐stable and reusable antifungal CA–DMP composite particles were superparamagnetic (49.79 emu g−1) and had an average diameter of 25.01 ± 1.36 nm. The novel composites showed good antifungal activity and excellent recycling performance, the sterilization rate of CA–DMPs remained above 96% after seven consecutive running cycles. CA–DMP composites could damage the fungal cell wall and membrane system, leading to the leakage of cell inclusions. Furthermore, transcriptome analysis of Aspergillus fumigatus treated with composites showed that 466 differentially expressed genes were primarily associated with cell wall membrane, membrane transporters, energy metabolism, genetic expression, and oxidation–reduction. The effect of CA–DMPs in inducing mitochondrial membrane dysfunction might result in the disruption of energy metabolism and REDOX homeostasis. Overall, the results reported herein provide new insight into the potential antifungal nanomaterials. In vitro antifungal experiments performed on cherry tomatoes confirm the application potential of the synthesized material in the field of fruit and vegetable preservation. |
| first_indexed | 2024-03-13T06:45:46Z |
| format | Article |
| id | doaj.art-68038012d1874d4b92f5e780d2ad28bb |
| institution | Directory Open Access Journal |
| issn | 2643-8429 |
| language | English |
| last_indexed | 2024-03-13T06:45:46Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj.art-68038012d1874d4b92f5e780d2ad28bb2023-06-08T06:14:55ZengWileyFood Frontiers2643-84292023-06-014286788210.1002/fft2.225A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatusZihan Song0Yanli Zhang1Yongrui Ti2Huitian Qiao3Chen Niu4Yuqian Ban5Xiaoxiao Wang6Yuqing Hou7Ruiwen Lu8Zihan Song9Research Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaHealthy food personalized manufacturing and food safety control, College of Food Science & Engineering Northwest University Xi'an Shaanxi ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaResearch Group of Postharvest Technology, State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing ChinaAbstract To prevent the generation of drug‐resistant fungi from long‐term exposure to microorganisms, cinnamic acid (CA), a natural effective antifungal agent, was successfully coupled with poly‐dopamine magnetic nanoparticles (CA–DMPs). Due to the low solubility of CA, the saturated solution of CA (1.61 mg/mL, 45°C) had no antifungal effect. Contrarily, CA–DMPs showed a good antifungal effect. The resulting heat‐stable and reusable antifungal CA–DMP composite particles were superparamagnetic (49.79 emu g−1) and had an average diameter of 25.01 ± 1.36 nm. The novel composites showed good antifungal activity and excellent recycling performance, the sterilization rate of CA–DMPs remained above 96% after seven consecutive running cycles. CA–DMP composites could damage the fungal cell wall and membrane system, leading to the leakage of cell inclusions. Furthermore, transcriptome analysis of Aspergillus fumigatus treated with composites showed that 466 differentially expressed genes were primarily associated with cell wall membrane, membrane transporters, energy metabolism, genetic expression, and oxidation–reduction. The effect of CA–DMPs in inducing mitochondrial membrane dysfunction might result in the disruption of energy metabolism and REDOX homeostasis. Overall, the results reported herein provide new insight into the potential antifungal nanomaterials. In vitro antifungal experiments performed on cherry tomatoes confirm the application potential of the synthesized material in the field of fruit and vegetable preservation.https://doi.org/10.1002/fft2.225antifungal activityAspergillus fumigatuscinnamic acidpoly‐dopamine magnetic nanoparticlestranscriptome |
| spellingShingle | Zihan Song Yanli Zhang Yongrui Ti Huitian Qiao Chen Niu Yuqian Ban Xiaoxiao Wang Yuqing Hou Ruiwen Lu Zihan Song A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus Food Frontiers antifungal activity Aspergillus fumigatus cinnamic acid poly‐dopamine magnetic nanoparticles transcriptome |
| title | A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus |
| title_full | A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus |
| title_fullStr | A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus |
| title_full_unstemmed | A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus |
| title_short | A magnetic nanocomposite combined with cinnamic acid for capture–inhibition–separation of Aspergillus fumigatus |
| title_sort | magnetic nanocomposite combined with cinnamic acid for capture inhibition separation of aspergillus fumigatus |
| topic | antifungal activity Aspergillus fumigatus cinnamic acid poly‐dopamine magnetic nanoparticles transcriptome |
| url | https://doi.org/10.1002/fft2.225 |
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