Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA
Invertebrate-derived DNA (iDNA) metabarcoding from carrion flies is a powerful, non-invasive tool that has value for assessing vertebrate diversity. However, unknowns exist around the factors that influence vertebrate detections, such as spatial limits to iDNA signals or if detections are influenced...
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| Format: | Article |
| Language: | English |
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The Royal Society
2024-04-01
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| Series: | Royal Society Open Science |
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| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsos.231286 |
| _version_ | 1797215227771617280 |
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| author | Kristen Fernandes Philip W. Bateman Benjamin J. Saunders Mark Gibberd Michael Bunce Kristine Bohmann Paul Nevill |
| author_facet | Kristen Fernandes Philip W. Bateman Benjamin J. Saunders Mark Gibberd Michael Bunce Kristine Bohmann Paul Nevill |
| author_sort | Kristen Fernandes |
| collection | DOAJ |
| description | Invertebrate-derived DNA (iDNA) metabarcoding from carrion flies is a powerful, non-invasive tool that has value for assessing vertebrate diversity. However, unknowns exist around the factors that influence vertebrate detections, such as spatial limits to iDNA signals or if detections are influenced by taxonomic class or estimated biomass of the vertebrates of interest. Using a bulk-collection method, we captured flies from within a zoo and along transects extending 4 km away from this location. From 920 flies, we detected 28 vertebrate species. Of the 28 detected species, we identified 9 species kept at the zoo, 8 mammals and 1 bird, but no reptiles. iDNA detections were highly geographically localized, and only a few zoo animals were detected outside the zoo setting. However, due to the low number of detections in our dataset, we found no influence of the taxonomic group or the estimated biomass of animals on their detectability. Our data suggest that iDNA detections from bulk-collected carrion flies, at least in urban settings in Australia, are predominantly determined by geographic proximity to the sampling location. This study presents an important step in understanding how iDNA techniques can be used in biodiversity monitoring. |
| first_indexed | 2024-04-24T11:26:43Z |
| format | Article |
| id | doaj.art-6803e9e0711942d5be54950e2bbab9bb |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-04-24T11:26:43Z |
| publishDate | 2024-04-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-6803e9e0711942d5be54950e2bbab9bb2024-04-10T14:08:21ZengThe Royal SocietyRoyal Society Open Science2054-57032024-04-0111410.1098/rsos.231286Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNAKristen Fernandes0Philip W. Bateman1Benjamin J. Saunders2Mark Gibberd3Michael Bunce4Kristine Bohmann5Paul Nevill6Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University , Bentley, Western Australia 6102, AustraliaBehavioural Ecology Laboratory, School of Molecular and Life Sciences, Curtin University , Bentley, Western Australia 6102, AustraliaSchool of Molecular and Life Sciences, Curtin University , Bentley, Western Australia 6102, AustraliaFood Agility CRC Ltd , Sydney, New South Wales 2000, AustraliaTrace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University , Bentley, Western Australia 6102, AustraliaSection for Molecular Ecology and Evolution, Faculty of Health and Medical Sciences, Globe Institute, University of Copenhagen , Copenhagen, DenmarkMBioMe - Mine Site Biomonitoring using eDNA Research Group, Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University , Bentley, Western Australia 6102, AustraliaInvertebrate-derived DNA (iDNA) metabarcoding from carrion flies is a powerful, non-invasive tool that has value for assessing vertebrate diversity. However, unknowns exist around the factors that influence vertebrate detections, such as spatial limits to iDNA signals or if detections are influenced by taxonomic class or estimated biomass of the vertebrates of interest. Using a bulk-collection method, we captured flies from within a zoo and along transects extending 4 km away from this location. From 920 flies, we detected 28 vertebrate species. Of the 28 detected species, we identified 9 species kept at the zoo, 8 mammals and 1 bird, but no reptiles. iDNA detections were highly geographically localized, and only a few zoo animals were detected outside the zoo setting. However, due to the low number of detections in our dataset, we found no influence of the taxonomic group or the estimated biomass of animals on their detectability. Our data suggest that iDNA detections from bulk-collected carrion flies, at least in urban settings in Australia, are predominantly determined by geographic proximity to the sampling location. This study presents an important step in understanding how iDNA techniques can be used in biodiversity monitoring.https://royalsocietypublishing.org/doi/10.1098/rsos.231286invertebrate-derived DNAmetabarcodingterrestrial vertebratebiomonitoring |
| spellingShingle | Kristen Fernandes Philip W. Bateman Benjamin J. Saunders Mark Gibberd Michael Bunce Kristine Bohmann Paul Nevill Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA Royal Society Open Science invertebrate-derived DNA metabarcoding terrestrial vertebrate biomonitoring |
| title | Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA |
| title_full | Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA |
| title_fullStr | Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA |
| title_full_unstemmed | Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA |
| title_short | Analysing the effects of distance, taxon and biomass on vertebrate detections using bulk-collected carrion fly iDNA |
| title_sort | analysing the effects of distance taxon and biomass on vertebrate detections using bulk collected carrion fly idna |
| topic | invertebrate-derived DNA metabarcoding terrestrial vertebrate biomonitoring |
| url | https://royalsocietypublishing.org/doi/10.1098/rsos.231286 |
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