Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India
Tuberculosis (TB) diagnosis in children still remains a challenge in developing countries. We analyze the performance of Xpert MTB/RIF assay for the diagnosis of pediatric TB under programmatic conditions. We retrospectively analyzed the performance of Xpert MTB/RIF assay from February 2016 to March...
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| Format: | Article |
| Language: | English |
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Springer
2020-01-01
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| Series: | Journal of Epidemiology and Global Health |
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| Online Access: | https://www.atlantis-press.com/article/125931901/view |
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| author | Rakesh Yadav Pankaj Vaidya Joseph L Mathew Sanjay Verma Rajiv Khaneja Priyanka Agarwal Pankaj Kumar Meenu Singh Sunil Sethi |
| author_facet | Rakesh Yadav Pankaj Vaidya Joseph L Mathew Sanjay Verma Rajiv Khaneja Priyanka Agarwal Pankaj Kumar Meenu Singh Sunil Sethi |
| author_sort | Rakesh Yadav |
| collection | DOAJ |
| description | Tuberculosis (TB) diagnosis in children still remains a challenge in developing countries. We analyze the performance of Xpert MTB/RIF assay for the diagnosis of pediatric TB under programmatic conditions. We retrospectively analyzed the performance of Xpert MTB/RIF assay from February 2016 to March 2018. A total 2678 samples from TB suspects below 14 years were received in the laboratory and were frontline tested by Xpert MTB/RIF assay according to the manufacturer’s instructions. If sample was sufficient, the smear microscopy and culture were performed as per standard World Health Organization’s guidelines. The smears and cultures were performed in 2178 and 588 samples, respectively. Among 2678 samples, 68 were rejected, Xpert MTB/RIF assay was positive in 357/2610 (13.6%) cases, while the smear was positive in 81/2178 (3.3%) cases. The sensitivity of smear and Xpert MTB/RIF when compared with culture was 24.6% (14.1–37.8%) and 81% (68.6–90.1%), respectively. The diagnostic accuracy of Xpert MTB/RIF and smear was 97.1% and 92.2%, respectively. Thirty samples (8.5%) were detected as rifampicin resistance by Xpert MTB/RIF assay. The Xpert MTB/RIF increased the detection rate up to fourfold when compared with smear microscopy. Xpert MTB/RIF assay is the most rapid, sensitive, and specific method for microbiological confirmation and rifampicin resistance detection in pediatric tuberculosis. |
| first_indexed | 2024-04-13T20:41:47Z |
| format | Article |
| id | doaj.art-6804f47ca1b34636b53233656d49526a |
| institution | Directory Open Access Journal |
| issn | 2210-6014 |
| language | English |
| last_indexed | 2024-04-13T20:41:47Z |
| publishDate | 2020-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Epidemiology and Global Health |
| spelling | doaj.art-6804f47ca1b34636b53233656d49526a2022-12-22T02:30:50ZengSpringerJournal of Epidemiology and Global Health2210-60142020-01-0110210.2991/jegh.k.191215.002Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in IndiaRakesh YadavPankaj VaidyaJoseph L MathewSanjay VermaRajiv KhanejaPriyanka AgarwalPankaj KumarMeenu SinghSunil SethiTuberculosis (TB) diagnosis in children still remains a challenge in developing countries. We analyze the performance of Xpert MTB/RIF assay for the diagnosis of pediatric TB under programmatic conditions. We retrospectively analyzed the performance of Xpert MTB/RIF assay from February 2016 to March 2018. A total 2678 samples from TB suspects below 14 years were received in the laboratory and were frontline tested by Xpert MTB/RIF assay according to the manufacturer’s instructions. If sample was sufficient, the smear microscopy and culture were performed as per standard World Health Organization’s guidelines. The smears and cultures were performed in 2178 and 588 samples, respectively. Among 2678 samples, 68 were rejected, Xpert MTB/RIF assay was positive in 357/2610 (13.6%) cases, while the smear was positive in 81/2178 (3.3%) cases. The sensitivity of smear and Xpert MTB/RIF when compared with culture was 24.6% (14.1–37.8%) and 81% (68.6–90.1%), respectively. The diagnostic accuracy of Xpert MTB/RIF and smear was 97.1% and 92.2%, respectively. Thirty samples (8.5%) were detected as rifampicin resistance by Xpert MTB/RIF assay. The Xpert MTB/RIF increased the detection rate up to fourfold when compared with smear microscopy. Xpert MTB/RIF assay is the most rapid, sensitive, and specific method for microbiological confirmation and rifampicin resistance detection in pediatric tuberculosis.https://www.atlantis-press.com/article/125931901/viewXpert MTB/RIFpediatric tuberculosisdiagnosisIndia |
| spellingShingle | Rakesh Yadav Pankaj Vaidya Joseph L Mathew Sanjay Verma Rajiv Khaneja Priyanka Agarwal Pankaj Kumar Meenu Singh Sunil Sethi Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India Journal of Epidemiology and Global Health Xpert MTB/RIF pediatric tuberculosis diagnosis India |
| title | Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India |
| title_full | Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India |
| title_fullStr | Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India |
| title_full_unstemmed | Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India |
| title_short | Utility of Xpert MTB/RIF Assay for Diagnosis of Pediatric Tuberculosis Under Programmatic Conditions in India |
| title_sort | utility of xpert mtb rif assay for diagnosis of pediatric tuberculosis under programmatic conditions in india |
| topic | Xpert MTB/RIF pediatric tuberculosis diagnosis India |
| url | https://www.atlantis-press.com/article/125931901/view |
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