Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress
At a sedimentary site in an old mine site, Miscanthus sinensis formed patches, where Pinus densiflora seedlings could grow better compared with those outside the patches, indicating that M. sinensis would improve P. densiflora seedling establishment. The purpose of this study was to understand the m...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204988/?tool=EBI |
| _version_ | 1797819815929315328 |
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| author | Toshikatsu Haruma Kohei Doyama Xingyan Lu Kenta Noji Hayato Masuya Takahiko Arima Shingo Tomiyama Keiko Yamaji |
| author_facet | Toshikatsu Haruma Kohei Doyama Xingyan Lu Kenta Noji Hayato Masuya Takahiko Arima Shingo Tomiyama Keiko Yamaji |
| author_sort | Toshikatsu Haruma |
| collection | DOAJ |
| description | At a sedimentary site in an old mine site, Miscanthus sinensis formed patches, where Pinus densiflora seedlings could grow better compared with those outside the patches, indicating that M. sinensis would improve P. densiflora seedling establishment. The purpose of this study was to understand the mechanisms by which M. sinensis facilitates the survival of P. densiflora seedlings by considering the soil properties, heavy metal tolerance, and root endophytes in P. densiflora seedlings at the sedimentary site. The sedimentary site, which is a bare ground, contained high concentrations of Fe, indicating that plants should be exposed to Fe and high soil temperature stresses. Measurement of soil temperature revealed that M. sinensis suppressed sharp increases and alternation of soil temperature, resulting in reducing high soil temperature stress in P. densiflora seedlings. To adapt to the Fe stress environment, P. densiflora outside and inside the patches produced Fe detoxicants, including catechin, condensed tannin, and malic acid. Ceratobasidium bicorne and Aquapteridospora sp. were commonly isolated from P. densiflora seedlings outside and inside the patches as root endophytes, which might enhance Fe tolerance in the seedlings. Aquapteridospora sp., which is considered as a dark-septate endophyte (DSE), was also isolated from the roots of M. sinensis, suggesting that M. sinensis might play a source of a root endophyte to P. densiflora seedlings. Ceratobasidium bicorne could be classified into root endophytes showing symbiosis and weak pathogenicity to host plants. Therefore, high soil temperature stress would weaken P. densiflora seedlings, causing root endophytic C. bicorne to appear pathogenic. We suggested that P. densiflora could adapt to the Fe stress environment via producing Fe detoxicants, and M. sinensis would facilitate the establishment of P. densiflora seedlings in the sedimentary site by providing a DSE, Aquapteridospora sp., and maintaining symbiosis of C. bicorne from high soil temperature stress. |
| first_indexed | 2024-03-13T09:28:12Z |
| format | Article |
| id | doaj.art-680893671a5e4dd4a46583b7ba18470e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-13T09:28:12Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-680893671a5e4dd4a46583b7ba18470e2023-05-26T05:30:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01185Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stressToshikatsu HarumaKohei DoyamaXingyan LuKenta NojiHayato MasuyaTakahiko ArimaShingo TomiyamaKeiko YamajiAt a sedimentary site in an old mine site, Miscanthus sinensis formed patches, where Pinus densiflora seedlings could grow better compared with those outside the patches, indicating that M. sinensis would improve P. densiflora seedling establishment. The purpose of this study was to understand the mechanisms by which M. sinensis facilitates the survival of P. densiflora seedlings by considering the soil properties, heavy metal tolerance, and root endophytes in P. densiflora seedlings at the sedimentary site. The sedimentary site, which is a bare ground, contained high concentrations of Fe, indicating that plants should be exposed to Fe and high soil temperature stresses. Measurement of soil temperature revealed that M. sinensis suppressed sharp increases and alternation of soil temperature, resulting in reducing high soil temperature stress in P. densiflora seedlings. To adapt to the Fe stress environment, P. densiflora outside and inside the patches produced Fe detoxicants, including catechin, condensed tannin, and malic acid. Ceratobasidium bicorne and Aquapteridospora sp. were commonly isolated from P. densiflora seedlings outside and inside the patches as root endophytes, which might enhance Fe tolerance in the seedlings. Aquapteridospora sp., which is considered as a dark-septate endophyte (DSE), was also isolated from the roots of M. sinensis, suggesting that M. sinensis might play a source of a root endophyte to P. densiflora seedlings. Ceratobasidium bicorne could be classified into root endophytes showing symbiosis and weak pathogenicity to host plants. Therefore, high soil temperature stress would weaken P. densiflora seedlings, causing root endophytic C. bicorne to appear pathogenic. We suggested that P. densiflora could adapt to the Fe stress environment via producing Fe detoxicants, and M. sinensis would facilitate the establishment of P. densiflora seedlings in the sedimentary site by providing a DSE, Aquapteridospora sp., and maintaining symbiosis of C. bicorne from high soil temperature stress.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204988/?tool=EBI |
| spellingShingle | Toshikatsu Haruma Kohei Doyama Xingyan Lu Kenta Noji Hayato Masuya Takahiko Arima Shingo Tomiyama Keiko Yamaji Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress PLoS ONE |
| title | Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress |
| title_full | Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress |
| title_fullStr | Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress |
| title_full_unstemmed | Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress |
| title_short | Miscanthus sinensis contributes to the survival of Pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between P. densiflora and endophytes from high soil temperature stress |
| title_sort | miscanthus sinensis contributes to the survival of pinus densiflora seedlings at a mining site via providing a possible functional endophyte and maintaining symbiotic relationship between p densiflora and endophytes from high soil temperature stress |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10204988/?tool=EBI |
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