Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods
Objective(s): Strain subtyping is an important epidemiological tool to trace contamination, determine clonal relationships between different strains, and the cause of outbreaks. Current subtyping methods, however, yield less than optimal subtype discrimination. Pulsed-field gel electrophoresis is th...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-04-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_17756_aa81eeea70129a50b505db72ad1c2685.pdf |
| _version_ | 1818833062130089984 |
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| author | Alireza Dolatyar Dehkharghani Setareh Haghighat Marjan Rahnamaye-Farzami Masoumeh Douraghi Mohammad Rahbar |
| author_facet | Alireza Dolatyar Dehkharghani Setareh Haghighat Marjan Rahnamaye-Farzami Masoumeh Douraghi Mohammad Rahbar |
| author_sort | Alireza Dolatyar Dehkharghani |
| collection | DOAJ |
| description | Objective(s): Strain subtyping is an important epidemiological tool to trace contamination, determine clonal relationships between different strains, and the cause of outbreaks. Current subtyping methods, however, yield less than optimal subtype discrimination. Pulsed-field gel electrophoresis is the gold standard method for Escherichia coli and Multiple-Locus Variable-number tandem repeat Analysis is a rapid PCR-based method. The purpose of this study was to evaluate MLVA and PFGE methods for subtyping β -lactamase-producing E. coli strains isolated from urinary tract infections.Materials and Methods: Overall, 230 E. coli isolates from patients with urinary tract infections were examined for antimicrobial susceptibility testing. 10-loci and 7-loci MLVA and PFGE methods were used for molecular typing of β -lactamase-producing E. coli isolates. Results: Out of 230 isolates, 130 (56.5%) β -lactamase-producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of 0.48 and 0.54 for 7-loci and 10-loci MLVA, respectively. The discriminatory power of PFGE showed a value of 0.87. The discordance between the methods was high. Conclusion: Our study showed that PFGE is more discriminatory than MVLA. MLVA is a PCR- based method and can generate unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA based on geographical region. |
| first_indexed | 2024-12-19T02:12:57Z |
| format | Article |
| id | doaj.art-680ad3b6018c41959be9e5c64ca35dd0 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-19T02:12:57Z |
| publishDate | 2021-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-680ad3b6018c41959be9e5c64ca35dd02022-12-21T20:40:37ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-04-0124443744310.22038/ijbms.2021.49790.1137217756Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methodsAlireza Dolatyar Dehkharghani0Setareh Haghighat1Marjan Rahnamaye-Farzami2Masoumeh Douraghi3Mohammad Rahbar4Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, IranDepartment of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, IranDepartment of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, IranDivision of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Microbiology, Research Center of Reference Health Laboratory, Ministry of Health and Medical Education, Tehran, IranObjective(s): Strain subtyping is an important epidemiological tool to trace contamination, determine clonal relationships between different strains, and the cause of outbreaks. Current subtyping methods, however, yield less than optimal subtype discrimination. Pulsed-field gel electrophoresis is the gold standard method for Escherichia coli and Multiple-Locus Variable-number tandem repeat Analysis is a rapid PCR-based method. The purpose of this study was to evaluate MLVA and PFGE methods for subtyping β -lactamase-producing E. coli strains isolated from urinary tract infections.Materials and Methods: Overall, 230 E. coli isolates from patients with urinary tract infections were examined for antimicrobial susceptibility testing. 10-loci and 7-loci MLVA and PFGE methods were used for molecular typing of β -lactamase-producing E. coli isolates. Results: Out of 230 isolates, 130 (56.5%) β -lactamase-producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of 0.48 and 0.54 for 7-loci and 10-loci MLVA, respectively. The discriminatory power of PFGE showed a value of 0.87. The discordance between the methods was high. Conclusion: Our study showed that PFGE is more discriminatory than MVLA. MLVA is a PCR- based method and can generate unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA based on geographical region.https://ijbms.mums.ac.ir/article_17756_aa81eeea70129a50b505db72ad1c2685.pdfbetalactamase escherichia coli molecular typing pulsedfield gel electrophoresis vntr |
| spellingShingle | Alireza Dolatyar Dehkharghani Setareh Haghighat Marjan Rahnamaye-Farzami Masoumeh Douraghi Mohammad Rahbar Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods Iranian Journal of Basic Medical Sciences beta lactamase escherichia coli molecular typing pulsed field gel electrophoresis vntr |
| title | Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods |
| title_full | Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods |
| title_fullStr | Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods |
| title_full_unstemmed | Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods |
| title_short | Subtyping β -lactamase-producing Escherichia coli strains isolated from patients with UTI by MLVA and PFGE methods |
| title_sort | subtyping β lactamase producing escherichia coli strains isolated from patients with uti by mlva and pfge methods |
| topic | beta lactamase escherichia coli molecular typing pulsed field gel electrophoresis vntr |
| url | https://ijbms.mums.ac.ir/article_17756_aa81eeea70129a50b505db72ad1c2685.pdf |
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