Further studies on the molecular systematics of Biomphalaria snails from Brazil
The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the a...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2000-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009&tlng=en |
| _version_ | 1797757042885132288 |
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| author | Teofânia HDA Vidigal Roberta Lima Caldeira Andrew JG Simpson Omar S Carvalho |
| author_facet | Teofânia HDA Vidigal Roberta Lima Caldeira Andrew JG Simpson Omar S Carvalho |
| author_sort | Teofânia HDA Vidigal |
| collection | DOAJ |
| description | The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snails |
| first_indexed | 2024-03-12T18:09:23Z |
| format | Article |
| id | doaj.art-680ae220c48a492eae8f1118fd35e74d |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:09:23Z |
| publishDate | 2000-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-680ae220c48a492eae8f1118fd35e74d2023-08-02T09:16:32ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602000-01-01951576610.1590/S0074-02762000000100009Further studies on the molecular systematics of Biomphalaria snails from BrazilTeofânia HDA Vidigal0Roberta Lima Caldeira1Andrew JG Simpson2Omar S Carvalho3FiocruzFiocruzInstituto Ludwig de Pesquisas sobre o CâncerFiocruzThe polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snailshttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009&tlng=enBiomphalariaribosomal DNAinternal transcribed spacerpolymerase chain reactionBrazil |
| spellingShingle | Teofânia HDA Vidigal Roberta Lima Caldeira Andrew JG Simpson Omar S Carvalho Further studies on the molecular systematics of Biomphalaria snails from Brazil Memorias do Instituto Oswaldo Cruz Biomphalaria ribosomal DNA internal transcribed spacer polymerase chain reaction Brazil |
| title | Further studies on the molecular systematics of Biomphalaria snails from Brazil |
| title_full | Further studies on the molecular systematics of Biomphalaria snails from Brazil |
| title_fullStr | Further studies on the molecular systematics of Biomphalaria snails from Brazil |
| title_full_unstemmed | Further studies on the molecular systematics of Biomphalaria snails from Brazil |
| title_short | Further studies on the molecular systematics of Biomphalaria snails from Brazil |
| title_sort | further studies on the molecular systematics of biomphalaria snails from brazil |
| topic | Biomphalaria ribosomal DNA internal transcribed spacer polymerase chain reaction Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762000000100009&tlng=en |
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