Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan)
A total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantit...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Oles Honchar Dnipro National University
2019-11-01
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| Series: | Regulatory Mechanisms in Biosystems |
| Subjects: | |
| Online Access: | https://medicine.dp.ua/index.php/med/article/view/560 |
| _version_ | 1827096193958674432 |
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| author | M. Ali M. Nisar W. Khan T. Naz S. U. Zaman M. Hussain |
| author_facet | M. Ali M. Nisar W. Khan T. Naz S. U. Zaman M. Hussain |
| author_sort | M. Ali |
| collection | DOAJ |
| description | A total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantitative traits were noted as per IPGRI, 1994 descriptor. Among the quantitative parameters, a high level of genetic variability index was noted in seeds per spike at 79.9% of coefficient of variance followed by biomass per plant which shows 37.4% variance, while minimum variance in quantitative traits was noted in days to germination at 5.4% followed by days to maturity at 3.1% with average mean genetic variation in all quantitative traits at 97.6%. Assay of total seed protein in these exotic accessions was analogue through polyacrylamide gel electrophoresis. A high level of variation was noted in loci (bands) B26 (0.98%) followed by B25 (0.89%), B24 (0.78%),B23 (0.69%) and B01 (0.52%). A similarly low level of variation was detected in B03 (0.16%) followed by B06 (0.18%), B13 (0.19%), B12 (0.21%), B11 (0.23%), B05 (0.24%), B07 (0.25%), B21 (0.34%), B20 (0.35%), B17 (0.39%). The results indicate that the mean value of variation in these accessions is 97.6%. Further assessments and exploration were suggested for these genotypes in multi-climatic zones to satisfy farmers’ need, breeders’ interest and malt-industrial requirements. |
| first_indexed | 2024-04-11T07:03:24Z |
| format | Article |
| id | doaj.art-680e0e5dbc1f49128138a4e8b6502121 |
| institution | Directory Open Access Journal |
| issn | 2519-8521 2520-2588 |
| language | English |
| last_indexed | 2025-03-20T07:11:53Z |
| publishDate | 2019-11-01 |
| publisher | Oles Honchar Dnipro National University |
| record_format | Article |
| series | Regulatory Mechanisms in Biosystems |
| spelling | doaj.art-680e0e5dbc1f49128138a4e8b65021212024-10-01T08:15:35ZengOles Honchar Dnipro National UniversityRegulatory Mechanisms in Biosystems2519-85212520-25882019-11-0110440040510.15421/021960560Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan)M. Ali0M. Nisar1W. Khan2T. Naz3S. U. Zaman4M. Hussain5Hazara UniversityUniversity of MalakandUniversity of MalakandUniversity of MalakandHazara UniversityHazara UniversityA total of 198 exotic barley genotypes were collected from the Gene Bank of the Plant Genetic Resource Institute (PGRI), National Agriculture Research Center (NARC), Islamabad, Pakistan, for the assessment of genetic diversity based on morphological and seed storage proteins. Qualitative and quantitative traits were noted as per IPGRI, 1994 descriptor. Among the quantitative parameters, a high level of genetic variability index was noted in seeds per spike at 79.9% of coefficient of variance followed by biomass per plant which shows 37.4% variance, while minimum variance in quantitative traits was noted in days to germination at 5.4% followed by days to maturity at 3.1% with average mean genetic variation in all quantitative traits at 97.6%. Assay of total seed protein in these exotic accessions was analogue through polyacrylamide gel electrophoresis. A high level of variation was noted in loci (bands) B26 (0.98%) followed by B25 (0.89%), B24 (0.78%),B23 (0.69%) and B01 (0.52%). A similarly low level of variation was detected in B03 (0.16%) followed by B06 (0.18%), B13 (0.19%), B12 (0.21%), B11 (0.23%), B05 (0.24%), B07 (0.25%), B21 (0.34%), B20 (0.35%), B17 (0.39%). The results indicate that the mean value of variation in these accessions is 97.6%. Further assessments and exploration were suggested for these genotypes in multi-climatic zones to satisfy farmers’ need, breeders’ interest and malt-industrial requirements.https://medicine.dp.ua/index.php/med/article/view/560hordeium vulgare; genetic variability; morphology; polyacrylamide gel electrophoresis. |
| spellingShingle | M. Ali M. Nisar W. Khan T. Naz S. U. Zaman M. Hussain Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan) Regulatory Mechanisms in Biosystems hordeium vulgare; genetic variability; morphology; polyacrylamide gel electrophoresis. |
| title | Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan) |
| title_full | Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan) |
| title_fullStr | Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan) |
| title_full_unstemmed | Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan) |
| title_short | Assessment of phenotypic and storage protein diversity in exotic barley cultivated in District Dir (Pakistan) |
| title_sort | assessment of phenotypic and storage protein diversity in exotic barley cultivated in district dir pakistan |
| topic | hordeium vulgare; genetic variability; morphology; polyacrylamide gel electrophoresis. |
| url | https://medicine.dp.ua/index.php/med/article/view/560 |
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