Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack
Multigenic families of Plant Defensin type 1 (PDF1) have been described in several species, including the model plant Arabidopsis thaliana as well as zinc tolerant and hyperaccumulator A. halleri. In A. thaliana, PDF1 transcripts (AtPDF1) accumulate in response to pathogen attack following synergic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00070/full |
| _version_ | 1818612107002773504 |
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| author | Nga N. T. eNGUYEN Vincent eRANWEZ Denis eVILE Marie Christine eSOULIE Alia eDELLAGI Dominique eEXPERT Françoise eGOSTI |
| author_facet | Nga N. T. eNGUYEN Vincent eRANWEZ Denis eVILE Marie Christine eSOULIE Alia eDELLAGI Dominique eEXPERT Françoise eGOSTI |
| author_sort | Nga N. T. eNGUYEN |
| collection | DOAJ |
| description | Multigenic families of Plant Defensin type 1 (PDF1) have been described in several species, including the model plant Arabidopsis thaliana as well as zinc tolerant and hyperaccumulator A. halleri. In A. thaliana, PDF1 transcripts (AtPDF1) accumulate in response to pathogen attack following synergic activation of ethylene/jasmonate pathways. However, in A. halleri, PDF1 transcripts (AhPDF1) are constitutively highly accumulated. Through an evolutionary approach, we investigated the possibility of A. halleri or A. thaliana species specialisation in different PDF1s in conveying zinc tolerance and/or the response to pathogen attack via activation of the jasmonate (JA) signalling pathway. The accumulation of each PDF1 from both A. halleri and A. thaliana was thus compared in response to zinc excess and MeJA application. In both species, PDF1 paralogues were barely or not at all responsive to zinc. However, regarding the PDF1 response to JA signalling activation, A. thaliana had a higher number of PDF1s responding to JA signalling activation. Remarkably, in A. thaliana, a slight but significant increase in zinc tolerance was correlated with activation of the JA signalling pathway. In addition, A. halleri was found to be more tolerant to the necrotrophic pathogen Botrytis cinerea than A. thaliana. Since PDF1s are known to be promiscuous antifungal proteins able to convey zinc tolerance, we propose, on the basis of the findings of this study, that high constitutive PDF1 transcript accumulation in A. halleri is a potential way to skip the JA signalling activation step required to increase the PDF1 transcript level in the A. thaliana model species. This could ultimately represent an adaptive evolutionary process that would promote a PDF1 joint effect on both zinc tolerance and the response to pathogens in the A. halleri extremophile species. |
| first_indexed | 2024-12-16T15:40:57Z |
| format | Article |
| id | doaj.art-680e58f3c65947d1b5a3d335c48d68a4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T15:40:57Z |
| publishDate | 2014-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-680e58f3c65947d1b5a3d335c48d68a42022-12-21T22:26:00ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-03-01510.3389/fpls.2014.0007060418Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen AttackNga N. T. eNGUYEN0Vincent eRANWEZ1Denis eVILE2Marie Christine eSOULIE3Alia eDELLAGI4Dominique eEXPERT5Françoise eGOSTI6Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche, Montpellier SupAgro/CNRS/INRA/Université Montpellier IIAmélioration Génétique et Adaptation des Plantes Méditerranéennes et Tropicales, Unité Mixte de RechercheLaboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, UMR759Université Pierre et Marie Curie (UPMC Univ. Paris 06)Unité Mixte de Recherche 217 INRA/AgroParisTech/UPMCUnité Mixte de Recherche 217 INRA/AgroParisTech/UPMCBiochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche, Montpellier SupAgro/CNRS/INRA/Université Montpellier IIMultigenic families of Plant Defensin type 1 (PDF1) have been described in several species, including the model plant Arabidopsis thaliana as well as zinc tolerant and hyperaccumulator A. halleri. In A. thaliana, PDF1 transcripts (AtPDF1) accumulate in response to pathogen attack following synergic activation of ethylene/jasmonate pathways. However, in A. halleri, PDF1 transcripts (AhPDF1) are constitutively highly accumulated. Through an evolutionary approach, we investigated the possibility of A. halleri or A. thaliana species specialisation in different PDF1s in conveying zinc tolerance and/or the response to pathogen attack via activation of the jasmonate (JA) signalling pathway. The accumulation of each PDF1 from both A. halleri and A. thaliana was thus compared in response to zinc excess and MeJA application. In both species, PDF1 paralogues were barely or not at all responsive to zinc. However, regarding the PDF1 response to JA signalling activation, A. thaliana had a higher number of PDF1s responding to JA signalling activation. Remarkably, in A. thaliana, a slight but significant increase in zinc tolerance was correlated with activation of the JA signalling pathway. In addition, A. halleri was found to be more tolerant to the necrotrophic pathogen Botrytis cinerea than A. thaliana. Since PDF1s are known to be promiscuous antifungal proteins able to convey zinc tolerance, we propose, on the basis of the findings of this study, that high constitutive PDF1 transcript accumulation in A. halleri is a potential way to skip the JA signalling activation step required to increase the PDF1 transcript level in the A. thaliana model species. This could ultimately represent an adaptive evolutionary process that would promote a PDF1 joint effect on both zinc tolerance and the response to pathogens in the A. halleri extremophile species.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00070/fullDefensinsGene DuplicationCross-talkArabidopsis hallerijoint effectszinc tolerance |
| spellingShingle | Nga N. T. eNGUYEN Vincent eRANWEZ Denis eVILE Marie Christine eSOULIE Alia eDELLAGI Dominique eEXPERT Françoise eGOSTI Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack Frontiers in Plant Science Defensins Gene Duplication Cross-talk Arabidopsis halleri joint effects zinc tolerance |
| title | Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack |
| title_full | Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack |
| title_fullStr | Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack |
| title_full_unstemmed | Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack |
| title_short | Evolutionary Tinkering of the Expression of PDF1s Suggests their Joint Effect on Zinc Tolerance and the Response To Pathogen Attack |
| title_sort | evolutionary tinkering of the expression of pdf1s suggests their joint effect on zinc tolerance and the response to pathogen attack |
| topic | Defensins Gene Duplication Cross-talk Arabidopsis halleri joint effects zinc tolerance |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00070/full |
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