Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys
Diseases caused by <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Salmonella</i> spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) <i>E. coli</i> and <i>Salmonella</i>...
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| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/11/770 |
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| author | Md. Tawyabur Md. Saiful Islam Md. Abdus Sobur Md. Jannat Hossain Md. Muket Mahmud Sumon Paul Muhammad Tofazzal Hossain Hossam M. Ashour Md. Tanvir Rahman |
| author_facet | Md. Tawyabur Md. Saiful Islam Md. Abdus Sobur Md. Jannat Hossain Md. Muket Mahmud Sumon Paul Muhammad Tofazzal Hossain Hossam M. Ashour Md. Tanvir Rahman |
| author_sort | Md. Tawyabur |
| collection | DOAJ |
| description | Diseases caused by <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Salmonella</i> spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) <i>E. coli</i> and <i>Salmonella</i> spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial isolation and identification were based on biochemical properties and polymerase chain reaction (PCR). Antibiogram profiles were determined by disk diffusion. The tetracycline-resistance gene <i>tetA</i> was detected by PCR. All samples were positive for <i>E. coli</i>. Only 11 samples (11/30; 36.67%) were positive for <i>Salmonella</i> spp. from healthy turkeys, whereas 16 (16/25; 64%) samples were positive for <i>Salmonella</i> spp. from diseased turkeys. <i>E. coli</i> isolated from diseased turkeys showed higher resistance to levofloxacin, gentamicin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. <i>Salmonella</i> spp. isolated from healthy turkeys exhibited higher resistance to gentamicin, chloramphenicol, ciprofloxacin, streptomycin, imipenem, and meropenem. All <i>E. coli</i> and <i>Salmonella</i> spp. from both healthy and diseased turkeys were resistant to erythromycin. <i>Salmonella</i> spp. from both healthy and diseased turkeys were resistant to tetracycline. Multidrug resistance was observed in both <i>E. coli</i> and <i>Salmonella</i> spp. from diseased turkeys. Finally, the <i>tetA</i> gene was detected in 93.1% of the <i>E. coli</i> isolates and in 92.59% of the <i>Salmonella</i> spp. isolates. To the best of our knowledge, this is the first study to isolate and characterize <i>tetA</i>-gene-containing MDR <i>E. coli</i> and <i>Salmonella</i> spp. from healthy and diseased turkeys in Bangladesh. Both microorganisms are of zoonotic significance and represent a significant public health challenge. |
| first_indexed | 2024-03-10T15:09:03Z |
| format | Article |
| id | doaj.art-680e6e7ac5f94c5f8a1717fcdee6fbf3 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T15:09:03Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-680e6e7ac5f94c5f8a1717fcdee6fbf32023-11-20T19:29:50ZengMDPI AGAntibiotics2079-63822020-11-0191177010.3390/antibiotics9110770Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased TurkeysMd. Tawyabur0Md. Saiful Islam1Md. Abdus Sobur2Md. Jannat Hossain3Md. Muket Mahmud4Sumon Paul5Muhammad Tofazzal Hossain6Hossam M. Ashour7Md. Tanvir Rahman8Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Integrative Biology, College of Arts and Sciences, University of South Florida, St. Petersburg, FL 33701, USADepartment of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDiseases caused by <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Salmonella</i> spp. can negatively impact turkey farming. The aim of this study was to isolate and characterize multidrug-resistant (MDR) <i>E. coli</i> and <i>Salmonella</i> spp. in healthy and diseased turkeys. A total of 30 fecal samples from healthy turkeys and 25 intestinal samples from diseased turkeys that died of enteritis were collected. Bacterial isolation and identification were based on biochemical properties and polymerase chain reaction (PCR). Antibiogram profiles were determined by disk diffusion. The tetracycline-resistance gene <i>tetA</i> was detected by PCR. All samples were positive for <i>E. coli</i>. Only 11 samples (11/30; 36.67%) were positive for <i>Salmonella</i> spp. from healthy turkeys, whereas 16 (16/25; 64%) samples were positive for <i>Salmonella</i> spp. from diseased turkeys. <i>E. coli</i> isolated from diseased turkeys showed higher resistance to levofloxacin, gentamicin, chloramphenicol, ciprofloxacin, streptomycin, and tetracycline. <i>Salmonella</i> spp. isolated from healthy turkeys exhibited higher resistance to gentamicin, chloramphenicol, ciprofloxacin, streptomycin, imipenem, and meropenem. All <i>E. coli</i> and <i>Salmonella</i> spp. from both healthy and diseased turkeys were resistant to erythromycin. <i>Salmonella</i> spp. from both healthy and diseased turkeys were resistant to tetracycline. Multidrug resistance was observed in both <i>E. coli</i> and <i>Salmonella</i> spp. from diseased turkeys. Finally, the <i>tetA</i> gene was detected in 93.1% of the <i>E. coli</i> isolates and in 92.59% of the <i>Salmonella</i> spp. isolates. To the best of our knowledge, this is the first study to isolate and characterize <i>tetA</i>-gene-containing MDR <i>E. coli</i> and <i>Salmonella</i> spp. from healthy and diseased turkeys in Bangladesh. Both microorganisms are of zoonotic significance and represent a significant public health challenge.https://www.mdpi.com/2079-6382/9/11/770avian colibacillosissalmonellosisantibiotic resistanceMDR<i>tetA</i>public health |
| spellingShingle | Md. Tawyabur Md. Saiful Islam Md. Abdus Sobur Md. Jannat Hossain Md. Muket Mahmud Sumon Paul Muhammad Tofazzal Hossain Hossam M. Ashour Md. Tanvir Rahman Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys Antibiotics avian colibacillosis salmonellosis antibiotic resistance MDR <i>tetA</i> public health |
| title | Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys |
| title_full | Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys |
| title_fullStr | Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys |
| title_full_unstemmed | Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys |
| title_short | Isolation and Characterization of Multidrug-Resistant <i>Escherichia coli</i> and <i>Salmonella</i> spp. from Healthy and Diseased Turkeys |
| title_sort | isolation and characterization of multidrug resistant i escherichia coli i and i salmonella i spp from healthy and diseased turkeys |
| topic | avian colibacillosis salmonellosis antibiotic resistance MDR <i>tetA</i> public health |
| url | https://www.mdpi.com/2079-6382/9/11/770 |
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