A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>Vasculogenic mimicry (VM), as a new blood supply for tumor growth and hematogenous metastases, has been recently described in highly aggressive human melanoma cells, etc. We previously reported VM in human gallbladder carcinomas and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-04-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://www.jeccr.com/content/30/1/46 |
| _version_ | 1811285100091932672 |
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| author | Zhang Wen Z Fan Yue Z Sun Wei Ge Chun Y |
| author_facet | Zhang Wen Z Fan Yue Z Sun Wei Ge Chun Y |
| author_sort | Zhang Wen Z |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Vasculogenic mimicry (VM), as a new blood supply for tumor growth and hematogenous metastases, has been recently described in highly aggressive human melanoma cells, etc. We previously reported VM in human gallbladder carcinomas and its clinical significance. In this study, we further studied histomorphology and hemodynamic of VM in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it>.</p> <p>Methods</p> <p>The invasive potential of human gallbladder carcinoma cell lines GBC-SD and SGC-996 were identified by Transwell membrane. The vasculogenic-like network structures and the signal intensities i.e. hemodynamic in gallbladder carcinomas stimulated via the three-dimensional matrix of GBC-SD or SGC-996 cells <it>in vitro</it>, the nude mouse xenografts of GBC-SD or SGC-996 cells <it>in vivo </it>were observed by immunohistochemistry (H&E staining and CD<sub>31</sub>-PAS double staining), electron microscopy and micro-MRA with HAS-Gd-DTPA, respectively.</p> <p>Results</p> <p>Highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells could form patterned networks containing hollow matrix channels. 85.7% (6/7) of GBC-SD nude mouse xenografts existed the evidence of VM, 5.7% (17/300) channels contained red blood cells among these tumor cell-lined vasculatures. GBC-SD xenografts showed multiple high-intensity spots similar with the intensity observed at tumor marginal, a result consistent with pathological VM.</p> <p>Conclusions</p> <p>VM existed in gallbladder carcinomas by both three-dimensional matrix of highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells <it>in vitro </it>and GBC-SD nude mouse xenografts <it>in vivo</it>.</p> |
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| id | doaj.art-6810cb0ee65e4c6fa0c55b90347ecb22 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-04-13T02:38:44Z |
| publishDate | 2011-04-01 |
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| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-6810cb0ee65e4c6fa0c55b90347ecb222022-12-22T03:06:16ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662011-04-013014610.1186/1756-9966-30-46A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it>Zhang Wen ZFan Yue ZSun WeiGe Chun Y<p>Abstract</p> <p>Background</p> <p>Vasculogenic mimicry (VM), as a new blood supply for tumor growth and hematogenous metastases, has been recently described in highly aggressive human melanoma cells, etc. We previously reported VM in human gallbladder carcinomas and its clinical significance. In this study, we further studied histomorphology and hemodynamic of VM in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it>.</p> <p>Methods</p> <p>The invasive potential of human gallbladder carcinoma cell lines GBC-SD and SGC-996 were identified by Transwell membrane. The vasculogenic-like network structures and the signal intensities i.e. hemodynamic in gallbladder carcinomas stimulated via the three-dimensional matrix of GBC-SD or SGC-996 cells <it>in vitro</it>, the nude mouse xenografts of GBC-SD or SGC-996 cells <it>in vivo </it>were observed by immunohistochemistry (H&E staining and CD<sub>31</sub>-PAS double staining), electron microscopy and micro-MRA with HAS-Gd-DTPA, respectively.</p> <p>Results</p> <p>Highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells could form patterned networks containing hollow matrix channels. 85.7% (6/7) of GBC-SD nude mouse xenografts existed the evidence of VM, 5.7% (17/300) channels contained red blood cells among these tumor cell-lined vasculatures. GBC-SD xenografts showed multiple high-intensity spots similar with the intensity observed at tumor marginal, a result consistent with pathological VM.</p> <p>Conclusions</p> <p>VM existed in gallbladder carcinomas by both three-dimensional matrix of highly aggressive GBC-SD or poorly aggressive SGC-996 cells preconditioned by highly aggressive GBC-SD cells <it>in vitro </it>and GBC-SD nude mouse xenografts <it>in vivo</it>.</p>http://www.jeccr.com/content/30/1/46Gallbladder neoplasmvasculogenic mimicry3-dimensional matrixXenograft modelHistomorphologyHemodynamic |
| spellingShingle | Zhang Wen Z Fan Yue Z Sun Wei Ge Chun Y A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it> Journal of Experimental & Clinical Cancer Research Gallbladder neoplasm vasculogenic mimicry 3-dimensional matrix Xenograft model Histomorphology Hemodynamic |
| title | A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it> |
| title_full | A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it> |
| title_fullStr | A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it> |
| title_full_unstemmed | A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it> |
| title_short | A pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas <it>in vivo </it>and <it>in vitro</it> |
| title_sort | pilot histomorphology and hemodynamic of vasculogenic mimicry in gallbladder carcinomas it in vivo it and it in vitro it |
| topic | Gallbladder neoplasm vasculogenic mimicry 3-dimensional matrix Xenograft model Histomorphology Hemodynamic |
| url | http://www.jeccr.com/content/30/1/46 |
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