Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening
Abstract Guava is a highly perishable fruit due to its intense metabolism during ripening, with a shelf life of up to five days at room temperature. The loss of firmness during ripening is caused by the activity of hydrolytic enzymes that promote dissolution of the pectin constituents of the cell w...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia de Alimentos (ITAL)
2018-05-01
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| Series: | Brazilian Journal of Food Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100435&lng=en&tlng=en |
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| author | Samira Haddad Spiller Tamara Rezende Marques Anderson Assaid Simão Mariana Aparecida Braga Lucimara Nazaré Silva Botelho Gustavo Henrique Andrade Machado Rodrigo Martins Fráguas Custódio Donizete dos Santos |
| author_facet | Samira Haddad Spiller Tamara Rezende Marques Anderson Assaid Simão Mariana Aparecida Braga Lucimara Nazaré Silva Botelho Gustavo Henrique Andrade Machado Rodrigo Martins Fráguas Custódio Donizete dos Santos |
| author_sort | Samira Haddad Spiller |
| collection | DOAJ |
| description | Abstract Guava is a highly perishable fruit due to its intense metabolism during ripening, with a shelf life of up to five days at room temperature. The loss of firmness during ripening is caused by the activity of hydrolytic enzymes that promote dissolution of the pectin constituents of the cell wall. Although guava is considered to be rich in pectin, the amounts reported in the literature do not exceed 2.4%, a content indicating it is not responsible for the firmness of guava. The aim of this study was to extract pectin from the guava pulp during 7 days of ripening by two methods (ethanol and EDTA extraction) and suggest modifications in the methods by adding to the extraction residue, cellulase and pectinase to degrade the cell wall structure of the fruit and obtain larger amounts of pectin, which would imply the participation of pectin in the maintenance of fruit firmness. It was possible to infer there were no differences in the pectin levels extracted by the two methods, due to sugar contamination. As from the new stage in the execution by the two methods, the extraction was more efficient: 9.10% of pectin with EDTA and 7.63% with ethanol. The pectin contents found were higher than those mentioned in the literature, better explaining their responsibility in fruit firmness. |
| first_indexed | 2024-12-19T08:41:54Z |
| format | Article |
| id | doaj.art-6814fc983d6640f49176788bf350fee7 |
| institution | Directory Open Access Journal |
| issn | 1981-6723 |
| language | English |
| last_indexed | 2024-12-19T08:41:54Z |
| publishDate | 2018-05-01 |
| publisher | Instituto de Tecnologia de Alimentos (ITAL) |
| record_format | Article |
| series | Brazilian Journal of Food Technology |
| spelling | doaj.art-6814fc983d6640f49176788bf350fee72022-12-21T20:28:54ZengInstituto de Tecnologia de Alimentos (ITAL)Brazilian Journal of Food Technology1981-67232018-05-0121010.1590/1981-6723.03217S1981-67232018000100435Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripeningSamira Haddad SpillerTamara Rezende MarquesAnderson Assaid SimãoMariana Aparecida BragaLucimara Nazaré Silva BotelhoGustavo Henrique Andrade MachadoRodrigo Martins FráguasCustódio Donizete dos SantosAbstract Guava is a highly perishable fruit due to its intense metabolism during ripening, with a shelf life of up to five days at room temperature. The loss of firmness during ripening is caused by the activity of hydrolytic enzymes that promote dissolution of the pectin constituents of the cell wall. Although guava is considered to be rich in pectin, the amounts reported in the literature do not exceed 2.4%, a content indicating it is not responsible for the firmness of guava. The aim of this study was to extract pectin from the guava pulp during 7 days of ripening by two methods (ethanol and EDTA extraction) and suggest modifications in the methods by adding to the extraction residue, cellulase and pectinase to degrade the cell wall structure of the fruit and obtain larger amounts of pectin, which would imply the participation of pectin in the maintenance of fruit firmness. It was possible to infer there were no differences in the pectin levels extracted by the two methods, due to sugar contamination. As from the new stage in the execution by the two methods, the extraction was more efficient: 9.10% of pectin with EDTA and 7.63% with ethanol. The pectin contents found were higher than those mentioned in the literature, better explaining their responsibility in fruit firmness.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100435&lng=en&tlng=enFirmezaEnzimas hidrolíticasArmazenamentoTemperatura ambiente |
| spellingShingle | Samira Haddad Spiller Tamara Rezende Marques Anderson Assaid Simão Mariana Aparecida Braga Lucimara Nazaré Silva Botelho Gustavo Henrique Andrade Machado Rodrigo Martins Fráguas Custódio Donizete dos Santos Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening Brazilian Journal of Food Technology Firmeza Enzimas hidrolíticas Armazenamento Temperatura ambiente |
| title | Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening |
| title_full | Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening |
| title_fullStr | Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening |
| title_full_unstemmed | Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening |
| title_short | Modifications in the methods to extract pectin from cv. “Pedro Sato” guavas during ripening |
| title_sort | modifications in the methods to extract pectin from cv pedro sato guavas during ripening |
| topic | Firmeza Enzimas hidrolíticas Armazenamento Temperatura ambiente |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1981-67232018000100435&lng=en&tlng=en |
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