Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo
Dental pulp cells (DPCs) represent good candidates for the regeneration of dental tissue. This study aimed to evaluate the growth and differentiation potential of DPCs cultured inside demineralized dentin tubules in vivo. Six green fluorescent protein-transgenic rats (body weight 100 g each) and th...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2020-11-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4614 |
| _version_ | 1797260941056802816 |
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| author | Sultan Zeb Khan Sana Mirza Samina Karim Takashi Inoue Mohammed S. Bin-Shuwaish Laila Al Deeb Khold Al Ahdal Rana S. Al-Hamdan Ahmed M. Maawadh Fahim Vohra Tariq Abduljabbar |
| author_facet | Sultan Zeb Khan Sana Mirza Samina Karim Takashi Inoue Mohammed S. Bin-Shuwaish Laila Al Deeb Khold Al Ahdal Rana S. Al-Hamdan Ahmed M. Maawadh Fahim Vohra Tariq Abduljabbar |
| author_sort | Sultan Zeb Khan |
| collection | DOAJ |
| description |
Dental pulp cells (DPCs) represent good candidates for the regeneration of dental tissue. This study aimed to evaluate the growth and differentiation potential of DPCs cultured inside demineralized dentin tubules in vivo. Six green fluorescent protein-transgenic rats (body weight 100 g each) and thirty-two Sprague-Dawley (SD) male rats (body weight 250 g each) were used for DPC collection and dentin tubules preparation and transplantation, respectively. Third-passage DPCs with or without collagen gels were loaded into demineralized dentin tubules. Both types of grafts were transplanted into the rectus abdominis muscles of SD rats and were harvested after 21 days. The expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteopontin (OPN), nestin, and dentin sialoprotein (DSP) was analyzed by immunohistochemistry. Histological analysis showed that DPCs in the collagen gel formed an osteodentin-like hard tissue matrix after 21 days. Increased positive immunoreactivity for ALP, BSP, OPN, nestin, and DSP was observed in experimental groups compared with control. Our results demonstrate that DPCs in collagen gel inside demineralized dentin tubules show increased growth and differentiation.
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| first_indexed | 2024-04-24T23:33:19Z |
| format | Article |
| id | doaj.art-681604b62e6a4c78a7217b250ce5b0db |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:33:19Z |
| publishDate | 2020-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-681604b62e6a4c78a7217b250ce5b0db2024-03-15T14:29:58ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2020-11-0120410.17305/bjbms.2020.4614Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivoSultan Zeb Khan0Sana Mirza1Samina Karim2Takashi Inoue3Mohammed S. Bin-Shuwaish4https://orcid.org/0000-0001-8127-0165Laila Al Deeb5Khold Al Ahdal6Rana S. Al-Hamdan7Ahmed M. Maawadh8Fahim Vohra9https://orcid.org/0000-0002-6204-7985Tariq Abduljabbar10Department of Clinical Pathophysiology, Graduate School of Tokyo Dental College, Tokyo, JapanDepartment of Oral Pathology, Faculty of Dentistry, Ziauddin University, Karachi, PakistanDepartment of Ophthalmology, Hayatabad Medical Complex, Khyber Girls Medical College, Peshawar, PakistanDepartment of Clinical Pathophysiology, Graduate School of Tokyo Dental College, Tokyo, JapanDepartment of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi ArabiaDepartment of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia Dental pulp cells (DPCs) represent good candidates for the regeneration of dental tissue. This study aimed to evaluate the growth and differentiation potential of DPCs cultured inside demineralized dentin tubules in vivo. Six green fluorescent protein-transgenic rats (body weight 100 g each) and thirty-two Sprague-Dawley (SD) male rats (body weight 250 g each) were used for DPC collection and dentin tubules preparation and transplantation, respectively. Third-passage DPCs with or without collagen gels were loaded into demineralized dentin tubules. Both types of grafts were transplanted into the rectus abdominis muscles of SD rats and were harvested after 21 days. The expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteopontin (OPN), nestin, and dentin sialoprotein (DSP) was analyzed by immunohistochemistry. Histological analysis showed that DPCs in the collagen gel formed an osteodentin-like hard tissue matrix after 21 days. Increased positive immunoreactivity for ALP, BSP, OPN, nestin, and DSP was observed in experimental groups compared with control. Our results demonstrate that DPCs in collagen gel inside demineralized dentin tubules show increased growth and differentiation. https://www.bjbms.org/ojs/index.php/bjbms/article/view/4614Collagen type I geldemineralized dentin tubulesdental pulp cellsosteogenesis |
| spellingShingle | Sultan Zeb Khan Sana Mirza Samina Karim Takashi Inoue Mohammed S. Bin-Shuwaish Laila Al Deeb Khold Al Ahdal Rana S. Al-Hamdan Ahmed M. Maawadh Fahim Vohra Tariq Abduljabbar Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo Biomolecules & Biomedicine Collagen type I gel demineralized dentin tubules dental pulp cells osteogenesis |
| title | Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo |
| title_full | Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo |
| title_fullStr | Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo |
| title_full_unstemmed | Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo |
| title_short | Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo |
| title_sort | immunohistochemical study of dental pulp cells with 3d collagen type i gel in demineralized dentin tubules in vivo |
| topic | Collagen type I gel demineralized dentin tubules dental pulp cells osteogenesis |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4614 |
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