Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone
Zearalenone (ZEN) is one of the most common mycotoxin contaminants in food. For food safety, an efficient and environmental-friendly approach to ZEN degradation is significant. In this study, an <i>Aspergillus niger</i> strain, FS10, was stimulated with 1.0 μg/mL ZEN for 24 h, repeating...
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MDPI AG
2021-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/13/10/720 |
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| author | Jian Ji Jian Yu Yang Yang Xiao Yuan Jia Yang Yinzhi Zhang Jiadi Sun Xiulan Sun |
| author_facet | Jian Ji Jian Yu Yang Yang Xiao Yuan Jia Yang Yinzhi Zhang Jiadi Sun Xiulan Sun |
| author_sort | Jian Ji |
| collection | DOAJ |
| description | Zearalenone (ZEN) is one of the most common mycotoxin contaminants in food. For food safety, an efficient and environmental-friendly approach to ZEN degradation is significant. In this study, an <i>Aspergillus niger</i> strain, FS10, was stimulated with 1.0 μg/mL ZEN for 24 h, repeating 5 times to obtain a stressed strain, Zearalenone-Stressed-FS10 (ZEN-S-FS10), with high degradation efficiency. The results show that the degradation rate of ZEN-S-FS10 to ZEN can be stabilized above 95%. Through metabolomics analysis of the metabolome difference of FS10 before and after ZEN stimulation, it was found that the change of metabolic profile may be the main reason for the increase in the degradation rate of ZEN. The optimization results of degradation conditions of ZEN-S-FS10 show that the degradation efficiency is the highest with a concentration of 10<sup>4</sup> CFU/mL and a period of 28 h. Finally, we analyzed the degradation products by UPLC-q-TOF, which shows that ZEN was degraded into two low-toxicity products: C<sub>18</sub>H<sub>22</sub>O<sub>8</sub>S (Zearalenone 4-sulfate) and C<sub>18</sub>H<sub>22</sub>O<sub>5</sub> ((E)-Zearalenone). This provides a wide range of possibilities for the industrial application of this strain. |
| first_indexed | 2024-03-10T06:09:27Z |
| format | Article |
| id | doaj.art-68161a04a7054ef9b7e42c1fd97fc608 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T06:09:27Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-68161a04a7054ef9b7e42c1fd97fc6082023-11-22T20:13:25ZengMDPI AGToxins2072-66512021-10-01131072010.3390/toxins13100720Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of ZearalenoneJian Ji0Jian Yu1Yang Yang2Xiao Yuan3Jia Yang4Yinzhi Zhang5Jiadi Sun6Xiulan Sun7State Key Laboratory of Food Science and Technology, School of Food Science and Technology of Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology of Jiangnan University, Wuxi 214122, ChinaCollege of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, ChinaGuangzhou GRG Metrology and Test Co., Ltd., Guangzhou 510630, ChinaYangzhou Center for Food and Drug Control, Yangzhou 225000, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology of Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology of Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology of Jiangnan University, Wuxi 214122, ChinaZearalenone (ZEN) is one of the most common mycotoxin contaminants in food. For food safety, an efficient and environmental-friendly approach to ZEN degradation is significant. In this study, an <i>Aspergillus niger</i> strain, FS10, was stimulated with 1.0 μg/mL ZEN for 24 h, repeating 5 times to obtain a stressed strain, Zearalenone-Stressed-FS10 (ZEN-S-FS10), with high degradation efficiency. The results show that the degradation rate of ZEN-S-FS10 to ZEN can be stabilized above 95%. Through metabolomics analysis of the metabolome difference of FS10 before and after ZEN stimulation, it was found that the change of metabolic profile may be the main reason for the increase in the degradation rate of ZEN. The optimization results of degradation conditions of ZEN-S-FS10 show that the degradation efficiency is the highest with a concentration of 10<sup>4</sup> CFU/mL and a period of 28 h. Finally, we analyzed the degradation products by UPLC-q-TOF, which shows that ZEN was degraded into two low-toxicity products: C<sub>18</sub>H<sub>22</sub>O<sub>8</sub>S (Zearalenone 4-sulfate) and C<sub>18</sub>H<sub>22</sub>O<sub>5</sub> ((E)-Zearalenone). This provides a wide range of possibilities for the industrial application of this strain.https://www.mdpi.com/2072-6651/13/10/720zearalenone<i>Aspergillus niger</i>biodegradation methodsdegradation productsfood safety |
| spellingShingle | Jian Ji Jian Yu Yang Yang Xiao Yuan Jia Yang Yinzhi Zhang Jiadi Sun Xiulan Sun Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone Toxins zearalenone <i>Aspergillus niger</i> biodegradation methods degradation products food safety |
| title | Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone |
| title_full | Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone |
| title_fullStr | Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone |
| title_full_unstemmed | Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone |
| title_short | Exploration on the Enhancement of Detoxification Ability of Zearalenone and Its Degradation Products of <i>Aspergillus niger</i> FS10 under Directional Stress of Zearalenone |
| title_sort | exploration on the enhancement of detoxification ability of zearalenone and its degradation products of i aspergillus niger i fs10 under directional stress of zearalenone |
| topic | zearalenone <i>Aspergillus niger</i> biodegradation methods degradation products food safety |
| url | https://www.mdpi.com/2072-6651/13/10/720 |
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