Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings
Abstract Background Developing high-performance sensing frameworks for diagnosing anaplastic changes is the subject of debate. The lack of on-time diagnosis in patients with suspicious cancers can affect the prognosis and survival rate. As a correlate, the emergence of de novo strategies for develop...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Cancer Nanotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12645-023-00201-x |
| _version_ | 1797836615202111488 |
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| author | Hassan Nasrollahpour Balal Khalilzadeh Reza Rahbarghazi Nevin Erk Mohammad-Reza Rashidi Abdolhossein Naseri |
| author_facet | Hassan Nasrollahpour Balal Khalilzadeh Reza Rahbarghazi Nevin Erk Mohammad-Reza Rashidi Abdolhossein Naseri |
| author_sort | Hassan Nasrollahpour |
| collection | DOAJ |
| description | Abstract Background Developing high-performance sensing frameworks for diagnosing anaplastic changes is the subject of debate. The lack of on-time diagnosis in patients with suspicious cancers can affect the prognosis and survival rate. As a correlate, the emergence of de novo strategies for developing transducing frameworks has an inevitable role in advanced biosensing. The combination of green chemistry procedures with eco-friendly and biocompatible materials is of high desirability in this context. The synthesis of new biocompatible and cost-effective nanomaterials to meet the emerging needs of rising demands appeals to new synthetic methodologies. Methods Here, we applied the electrochemical synthesis method to the fabrication of biocompatible and subtly governed Molybdenum trioxide/poly taurine nano-bio films to monitor human epidermal growth factor receptor-2 (HER-2) in sera from breast cancer patients. Morphological and elemental assessments were performed using a scanning electron microscope, energy-dispersive X-Ray spectroscopy, and dot mapping analyses. In addition, HER-2 immunohistochemistry (IHC) staining was performed on tissue samples, and data were compared to the values obtained by Molybdenum trioxide/poly taurine nano-bio films. Results We also noted our platform is eligible for feasible, rapid, and specific determination of HER-2 factor in human samples. The method had a lower limit of quantification of 0.000001 ng/mL and a linear dynamic range between 0.1 ng/mL and 0.000001 ng/mL. IHC imaging showed that the degree of anaplastic changes in breast samples (intensity of HER-2 factor) was closely associated with the intensity of signals obtained by our developed immunosensor. Conclusions According to the obtained desirable coordination with pathological studies, the designed biosensor has excellent capability to use as a reliable diagnostic tool in clinical laboratories. Graphical Abstract |
| first_indexed | 2024-04-09T15:12:51Z |
| format | Article |
| id | doaj.art-68164bec687e4d08ad69c62b2b4bd628 |
| institution | Directory Open Access Journal |
| issn | 1868-6958 1868-6966 |
| language | English |
| last_indexed | 2024-04-09T15:12:51Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Nanotechnology |
| spelling | doaj.art-68164bec687e4d08ad69c62b2b4bd6282023-04-30T11:07:26ZengBMCCancer Nanotechnology1868-69581868-69662023-04-0114111510.1186/s12645-023-00201-xDevelopment of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findingsHassan Nasrollahpour0Balal Khalilzadeh1Reza Rahbarghazi2Nevin Erk3Mohammad-Reza Rashidi4Abdolhossein Naseri5Department of Analytical Chemistry, Faculty of Chemistry, University of TabrizStem Cell Research Center, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesDepartment of Analytical Chemistry, Faculty of Pharmacy, Ankara UniversityDepartment of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical SciencesDepartment of Analytical Chemistry, Faculty of Chemistry, University of TabrizAbstract Background Developing high-performance sensing frameworks for diagnosing anaplastic changes is the subject of debate. The lack of on-time diagnosis in patients with suspicious cancers can affect the prognosis and survival rate. As a correlate, the emergence of de novo strategies for developing transducing frameworks has an inevitable role in advanced biosensing. The combination of green chemistry procedures with eco-friendly and biocompatible materials is of high desirability in this context. The synthesis of new biocompatible and cost-effective nanomaterials to meet the emerging needs of rising demands appeals to new synthetic methodologies. Methods Here, we applied the electrochemical synthesis method to the fabrication of biocompatible and subtly governed Molybdenum trioxide/poly taurine nano-bio films to monitor human epidermal growth factor receptor-2 (HER-2) in sera from breast cancer patients. Morphological and elemental assessments were performed using a scanning electron microscope, energy-dispersive X-Ray spectroscopy, and dot mapping analyses. In addition, HER-2 immunohistochemistry (IHC) staining was performed on tissue samples, and data were compared to the values obtained by Molybdenum trioxide/poly taurine nano-bio films. Results We also noted our platform is eligible for feasible, rapid, and specific determination of HER-2 factor in human samples. The method had a lower limit of quantification of 0.000001 ng/mL and a linear dynamic range between 0.1 ng/mL and 0.000001 ng/mL. IHC imaging showed that the degree of anaplastic changes in breast samples (intensity of HER-2 factor) was closely associated with the intensity of signals obtained by our developed immunosensor. Conclusions According to the obtained desirable coordination with pathological studies, the designed biosensor has excellent capability to use as a reliable diagnostic tool in clinical laboratories. Graphical Abstracthttps://doi.org/10.1186/s12645-023-00201-xBreast cancerPathological changesMolybdenumPoly taurineNano-biocomposite |
| spellingShingle | Hassan Nasrollahpour Balal Khalilzadeh Reza Rahbarghazi Nevin Erk Mohammad-Reza Rashidi Abdolhossein Naseri Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings Cancer Nanotechnology Breast cancer Pathological changes Molybdenum Poly taurine Nano-biocomposite |
| title | Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings |
| title_full | Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings |
| title_fullStr | Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings |
| title_full_unstemmed | Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings |
| title_short | Development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano-biofilms confirmed pathological findings |
| title_sort | development of an electrochemical biosensor for the detection of mammary gland carcinoma using molybdenum enhanced poly taurine nano biofilms confirmed pathological findings |
| topic | Breast cancer Pathological changes Molybdenum Poly taurine Nano-biocomposite |
| url | https://doi.org/10.1186/s12645-023-00201-x |
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