Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study
The establishment of a stable animal model for intrauterine adhesion (IUA) can significantly enhance research on the pathogenesis and pathological changes of this disease, as well as on the development of innovative therapeutic approaches. In this study, three different modeling methods, including p...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024013963 |
| _version_ | 1797304482144452608 |
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| author | Peng-Cheng Liu Yu-Ting Song Long-Mei Zhao Yan-Ling Jiang Jun-Gen Hu Li Dong Xing-li Zhou Li Zhou Yaxing Li Jesse Li-Ling Hui-Qi Xie |
| author_facet | Peng-Cheng Liu Yu-Ting Song Long-Mei Zhao Yan-Ling Jiang Jun-Gen Hu Li Dong Xing-li Zhou Li Zhou Yaxing Li Jesse Li-Ling Hui-Qi Xie |
| author_sort | Peng-Cheng Liu |
| collection | DOAJ |
| description | The establishment of a stable animal model for intrauterine adhesion (IUA) can significantly enhance research on the pathogenesis and pathological changes of this disease, as well as on the development of innovative therapeutic approaches. In this study, three different modeling methods, including phenol mucilage combined mechanical scraping, ethanol combined mechanical scraping and ethanol modeling alone were designed. The morphological characteristics of the models were evaluated. The underlying mechanisms and fertility capacity of the ethanol modeling group were analyzed and compared to those of the sham surgery group. All three methods resulted in severe intrauterine adhesions, with ethanol being identified as a reliable modeling agent and was subsequently subjected to further evaluation. Immunohistochemistry and RT-PCR results indicated that the ethanol modeling group exhibited an increase in the degree of fibrosis and inflammation, as well as a significant reduction in endometrial thickness, gland number, vascularization, and endometrial receptivity, ultimately resulting in the loss of fertility capacity. The aforementioned findings indicate that the intrauterine perfusion of 95 % ethanol is efficacious in inducing the development of intrauterine adhesions in rats. Given its cost-effectiveness, efficacy, and stability in IUA formation, the use of 95 % ethanol intrauterine perfusion may serve as a novel platform for evaluating innovative anti-adhesion materials and bioengineered therapies. |
| first_indexed | 2024-03-08T00:10:07Z |
| format | Article |
| id | doaj.art-6817eaeb2e2e48c1a22d4699a7b67135 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:10:07Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-6817eaeb2e2e48c1a22d4699a7b671352024-02-17T06:40:51ZengElsevierHeliyon2405-84402024-02-01103e25365Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary studyPeng-Cheng Liu0Yu-Ting Song1Long-Mei Zhao2Yan-Ling Jiang3Jun-Gen Hu4Li Dong5Xing-li Zhou6Li Zhou7Yaxing Li8Jesse Li-Ling9Hui-Qi Xie10Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaRegenerative Medicine Research Center of Topregmed, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, ChinaResearch Core Facility of West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, ChinaDepartment of Medical Genetics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China; Frontier Medical Center, Tianfu Jincheng Laboratory, Chengdu, Sichuan, China; Corresponding author. Department of Orthopedic Surgery and Orthopedic Research Institute, Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, China.The establishment of a stable animal model for intrauterine adhesion (IUA) can significantly enhance research on the pathogenesis and pathological changes of this disease, as well as on the development of innovative therapeutic approaches. In this study, three different modeling methods, including phenol mucilage combined mechanical scraping, ethanol combined mechanical scraping and ethanol modeling alone were designed. The morphological characteristics of the models were evaluated. The underlying mechanisms and fertility capacity of the ethanol modeling group were analyzed and compared to those of the sham surgery group. All three methods resulted in severe intrauterine adhesions, with ethanol being identified as a reliable modeling agent and was subsequently subjected to further evaluation. Immunohistochemistry and RT-PCR results indicated that the ethanol modeling group exhibited an increase in the degree of fibrosis and inflammation, as well as a significant reduction in endometrial thickness, gland number, vascularization, and endometrial receptivity, ultimately resulting in the loss of fertility capacity. The aforementioned findings indicate that the intrauterine perfusion of 95 % ethanol is efficacious in inducing the development of intrauterine adhesions in rats. Given its cost-effectiveness, efficacy, and stability in IUA formation, the use of 95 % ethanol intrauterine perfusion may serve as a novel platform for evaluating innovative anti-adhesion materials and bioengineered therapies.http://www.sciencedirect.com/science/article/pii/S2405844024013963Intrauterine adhesionAnimal modelEthanol perfusionChemical injury |
| spellingShingle | Peng-Cheng Liu Yu-Ting Song Long-Mei Zhao Yan-Ling Jiang Jun-Gen Hu Li Dong Xing-li Zhou Li Zhou Yaxing Li Jesse Li-Ling Hui-Qi Xie Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study Heliyon Intrauterine adhesion Animal model Ethanol perfusion Chemical injury |
| title | Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study |
| title_full | Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study |
| title_fullStr | Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study |
| title_full_unstemmed | Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study |
| title_short | Establishment and comparison of different procedures for modeling intrauterine adhesion in rats: A preliminary study |
| title_sort | establishment and comparison of different procedures for modeling intrauterine adhesion in rats a preliminary study |
| topic | Intrauterine adhesion Animal model Ethanol perfusion Chemical injury |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024013963 |
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