Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy
Background: It is still uncertain if a dysregulated expression of activating Fc gamma receptors (FcγRs) is associated with the development of immunoglobulin A nephropathy (IgAN). Methods: RNA sequencing was used to determine the mRNA levels of type I FcγRs, which were then verified by quantitative r...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2022-06-01
|
| Series: | Therapeutic Advances in Chronic Disease |
| Online Access: | https://doi.org/10.1177/20406223221106878 |
| _version_ | 1811237818044776448 |
|---|---|
| author | Hongfen Li Youxia Liu Huyan Yu Fanghao Wang Junya Jia Tiekun Yan Shan Lin |
| author_facet | Hongfen Li Youxia Liu Huyan Yu Fanghao Wang Junya Jia Tiekun Yan Shan Lin |
| author_sort | Hongfen Li |
| collection | DOAJ |
| description | Background: It is still uncertain if a dysregulated expression of activating Fc gamma receptors (FcγRs) is associated with the development of immunoglobulin A nephropathy (IgAN). Methods: RNA sequencing was used to determine the mRNA levels of type I FcγRs, which were then verified by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). Commercial ELISA kits were used to detect plasma soluble FcγRIIIb (sFcγRIIIb). Results: We first examined the expression of FcγRs genes in 17 patients with IgAN and six healthy controls. The expression of FcγRIa, FcγRIb, FcγRIIa, FcγRIIc, FcγRIIIa, and FcγRIIIb was shown to be higher in IgAN patients. Even without statistical significance, there was a downward trend in FcγRIIb mRNA levels in IgAN. We observed that the expression levels of activating FcγR mRNAs were consistently higher in an independent set of 20 IgAN patients and 20 healthy controls, confirming the RNA-seq results. FcγRIIIb was the IgG receptor with the greatest difference in expression between the two groups (log 2 fold-change = 1.82). We observed a much higher percent of FcγRIIIb positive cells in IgAN by flow cytometry. Next, we measured plasma sFcRIIIb levels in 50 patients with IgAN and 50 healthy controls. The findings revealed that the mean sFcγRIIIb level in plasma in participants with IgAN was much higher than that of healthy controls. Increased sFcγRIIIb levels were associated with a substantial increase in body mass index (BMI), lipid levels, serum creatinine level, and a larger percentage of sclerosis compared with lower sFcRIIIb levels. Patients in the group with higher sFcγRIIIb levels were more likely to get glucocorticoid treatment. Conclusion: The results demonstrated that the mRNA levels of the activating Fc receptor of IgG were significantly increased in IgAN. Patients with higher plasma sFcγRIIIb levels may have had more severe illness than those with lower levels. |
| first_indexed | 2024-04-12T12:29:46Z |
| format | Article |
| id | doaj.art-68184fed36304df8adfd8dd57e3ddd90 |
| institution | Directory Open Access Journal |
| issn | 2040-6231 |
| language | English |
| last_indexed | 2024-04-12T12:29:46Z |
| publishDate | 2022-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Therapeutic Advances in Chronic Disease |
| spelling | doaj.art-68184fed36304df8adfd8dd57e3ddd902022-12-22T03:33:03ZengSAGE PublishingTherapeutic Advances in Chronic Disease2040-62312022-06-011310.1177/20406223221106878Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathyHongfen LiYouxia LiuHuyan YuFanghao WangJunya JiaTiekun YanShan LinBackground: It is still uncertain if a dysregulated expression of activating Fc gamma receptors (FcγRs) is associated with the development of immunoglobulin A nephropathy (IgAN). Methods: RNA sequencing was used to determine the mRNA levels of type I FcγRs, which were then verified by quantitative reverse transcription–polymerase chain reaction (qRT-PCR). Commercial ELISA kits were used to detect plasma soluble FcγRIIIb (sFcγRIIIb). Results: We first examined the expression of FcγRs genes in 17 patients with IgAN and six healthy controls. The expression of FcγRIa, FcγRIb, FcγRIIa, FcγRIIc, FcγRIIIa, and FcγRIIIb was shown to be higher in IgAN patients. Even without statistical significance, there was a downward trend in FcγRIIb mRNA levels in IgAN. We observed that the expression levels of activating FcγR mRNAs were consistently higher in an independent set of 20 IgAN patients and 20 healthy controls, confirming the RNA-seq results. FcγRIIIb was the IgG receptor with the greatest difference in expression between the two groups (log 2 fold-change = 1.82). We observed a much higher percent of FcγRIIIb positive cells in IgAN by flow cytometry. Next, we measured plasma sFcRIIIb levels in 50 patients with IgAN and 50 healthy controls. The findings revealed that the mean sFcγRIIIb level in plasma in participants with IgAN was much higher than that of healthy controls. Increased sFcγRIIIb levels were associated with a substantial increase in body mass index (BMI), lipid levels, serum creatinine level, and a larger percentage of sclerosis compared with lower sFcRIIIb levels. Patients in the group with higher sFcγRIIIb levels were more likely to get glucocorticoid treatment. Conclusion: The results demonstrated that the mRNA levels of the activating Fc receptor of IgG were significantly increased in IgAN. Patients with higher plasma sFcγRIIIb levels may have had more severe illness than those with lower levels.https://doi.org/10.1177/20406223221106878 |
| spellingShingle | Hongfen Li Youxia Liu Huyan Yu Fanghao Wang Junya Jia Tiekun Yan Shan Lin Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy Therapeutic Advances in Chronic Disease |
| title | Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy |
| title_full | Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy |
| title_fullStr | Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy |
| title_full_unstemmed | Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy |
| title_short | Elevated activating Fc gamma receptors levels correlated with susceptibility and severity of IgA nephropathy |
| title_sort | elevated activating fc gamma receptors levels correlated with susceptibility and severity of iga nephropathy |
| url | https://doi.org/10.1177/20406223221106878 |
| work_keys_str_mv | AT hongfenli elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy AT youxialiu elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy AT huyanyu elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy AT fanghaowang elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy AT junyajia elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy AT tiekunyan elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy AT shanlin elevatedactivatingfcgammareceptorslevelscorrelatedwithsusceptibilityandseverityofiganephropathy |