The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts
This study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT), consisting of 5% 5-aminolevulinic acid-gel and 630 nm-LED, already used for antibacterial effects in the treatment of periodontitis, on human gingival fibroblasts (HGF) and primary human osteoblasts (HOB). HGF and HO...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Online Access: | https://www.mdpi.com/2310-2861/8/8/491 |
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| author | Tania Vanessa Pierfelice Emira D’Amico Morena Petrini Assunta Pandolfi Camillo D’Arcangelo Natalia Di Pietro Adriano Piattelli Giovanna Iezzi |
| author_facet | Tania Vanessa Pierfelice Emira D’Amico Morena Petrini Assunta Pandolfi Camillo D’Arcangelo Natalia Di Pietro Adriano Piattelli Giovanna Iezzi |
| author_sort | Tania Vanessa Pierfelice |
| collection | DOAJ |
| description | This study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT), consisting of 5% 5-aminolevulinic acid-gel and 630 nm-LED, already used for antibacterial effects in the treatment of periodontitis, on human gingival fibroblasts (HGF) and primary human osteoblasts (HOB). HGF and HOB were incubated with different ALAD concentrations for 45 min, and subsequently irradiated with 630 nm-LED for 7 min. Firstly, the cytotoxicity at 24 h and proliferation at 48 and 72 h were assessed. Then the intracellular content of the protoporphyrin IX (PpIX) of the ROS and the superoxide dismutase (SOD) activity were investigated at different times. Each result was compared with untreated and unirradiated cells as the control. Viable and metabolic active cells were revealed at any concentrations of ALAD-PDT, but only 100-ALAD-PDT significantly enhanced the proliferation rate. The PpIX fluorescence significantly increased after the addition of 100-ALAD, and decreased after the irradiation. Higher ROS generation was detected at 10 min in HGF, and at 30 min in HOB. The activity of the SOD enzyme augmented at 30 min in both cell types. In conclusion, ALAD-PDT not only showed no cytotoxic effects, but had pro-proliferative effects on HGF and HOB, probably via ROS generation. |
| first_indexed | 2024-03-09T04:24:52Z |
| format | Article |
| id | doaj.art-681860daa59644bdbb4358daa7b69b53 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-09T04:24:52Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-681860daa59644bdbb4358daa7b69b532023-12-03T13:42:10ZengMDPI AGGels2310-28612022-08-018849110.3390/gels8080491The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and OsteoblastsTania Vanessa Pierfelice0Emira D’Amico1Morena Petrini2Assunta Pandolfi3Camillo D’Arcangelo4Natalia Di Pietro5Adriano Piattelli6Giovanna Iezzi7Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalySchool of Dentistry, Saint Camillus International University of Health and Medical Sciences, Via di Sant’Alessandro 8, 00131 Rome, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio of Chieti-Pescara, 66100 Chieti, ItalyThis study aimed to evaluate the effects of a new photodynamic protocol (ALAD-PDT), consisting of 5% 5-aminolevulinic acid-gel and 630 nm-LED, already used for antibacterial effects in the treatment of periodontitis, on human gingival fibroblasts (HGF) and primary human osteoblasts (HOB). HGF and HOB were incubated with different ALAD concentrations for 45 min, and subsequently irradiated with 630 nm-LED for 7 min. Firstly, the cytotoxicity at 24 h and proliferation at 48 and 72 h were assessed. Then the intracellular content of the protoporphyrin IX (PpIX) of the ROS and the superoxide dismutase (SOD) activity were investigated at different times. Each result was compared with untreated and unirradiated cells as the control. Viable and metabolic active cells were revealed at any concentrations of ALAD-PDT, but only 100-ALAD-PDT significantly enhanced the proliferation rate. The PpIX fluorescence significantly increased after the addition of 100-ALAD, and decreased after the irradiation. Higher ROS generation was detected at 10 min in HGF, and at 30 min in HOB. The activity of the SOD enzyme augmented at 30 min in both cell types. In conclusion, ALAD-PDT not only showed no cytotoxic effects, but had pro-proliferative effects on HGF and HOB, probably via ROS generation.https://www.mdpi.com/2310-2861/8/8/4915-delta aminolevulinic acidphotodynamic therapyperiodontal tissuesprotoporphyrinreactive oxygen species |
| spellingShingle | Tania Vanessa Pierfelice Emira D’Amico Morena Petrini Assunta Pandolfi Camillo D’Arcangelo Natalia Di Pietro Adriano Piattelli Giovanna Iezzi The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts Gels 5-delta aminolevulinic acid photodynamic therapy periodontal tissues protoporphyrin reactive oxygen species |
| title | The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts |
| title_full | The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts |
| title_fullStr | The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts |
| title_full_unstemmed | The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts |
| title_short | The Effects of 5% 5-Aminolevulinic Acid Gel and Red Light (ALAD-PDT) on Human Fibroblasts and Osteoblasts |
| title_sort | effects of 5 5 aminolevulinic acid gel and red light alad pdt on human fibroblasts and osteoblasts |
| topic | 5-delta aminolevulinic acid photodynamic therapy periodontal tissues protoporphyrin reactive oxygen species |
| url | https://www.mdpi.com/2310-2861/8/8/491 |
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