Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece
Objectives Vaccination against SARS-CoV-2 has been extensively deployed during COVID-19 pandemic. One efficient method to evaluate response to vaccination is the assessment of humoral immunity by measuring SARS-CoV-2 antibody titres. We investigated the association between anthropometric parameters...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2022-05-01
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| Series: | BMJ Open |
| Online Access: | https://bmjopen.bmj.com/content/12/5/e057084.full |
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| author | Markos Marangos Anastasia Parthymou Evagelia E Habeos George I Habeos Apostolos Deligakis Ektoras Livieratos Dionysios V Chartoumpekis |
| author_facet | Markos Marangos Anastasia Parthymou Evagelia E Habeos George I Habeos Apostolos Deligakis Ektoras Livieratos Dionysios V Chartoumpekis |
| author_sort | Markos Marangos |
| collection | DOAJ |
| description | Objectives Vaccination against SARS-CoV-2 has been extensively deployed during COVID-19 pandemic. One efficient method to evaluate response to vaccination is the assessment of humoral immunity by measuring SARS-CoV-2 antibody titres. We investigated the association between anthropometric parameters (age, body mass index), smoking, diabetes, statin use, hypertension, levels of 25(OH)D and dehydroepiandrosterone sulfate (DHEAS), and SARS-CoV-2 antibody titres after vaccination.Design In this longitudinal observational cohort study, 712 subjects were tested for SARS-CoV-2 antibodies 3 months after the second dose of BNT162b2 vaccine. Multiple linear regression analysis was performed to identify which factors are associated with the antibody titres.Setting Healthcare units of western Greece (University Hospital of Patras and “St Andrews” State General Hospital of Patras).Participants All adults receiving their second dose of BNT162b2 vaccine at the participating healthcare units were eligible to participate in the study. Exclusion criteria were SARS-CoV-2 infection or positive SARS-CoV-2 antibody titre at baseline. Patients who did not provide all necessary information were excluded from our analyses.Results We found age to be negatively associated with antibody titre (−0.005; 95% CI −0.009 to −0.001, p=0.0073), as was male gender (−0.11; 95% CI −0.1738 to −0.04617, p=0.0008). The interaction of age and gender was significant (−0.01090; 95% CI −0.01631 to −0.005490, p<0.0001), highlighting that the rate of decline in antibody titre with increasing age tends to be higher in men rather than in women. No linear trend was found between DHEAS levels and antibody titres when the lower quartile of DHEAS levels was used as reference. Tobacco use was associated with low antibody titre (−0.1097; 95% CI −0.174 to −0.046, p=0.0008) but overweight, obese or underweight subjects had similar antibody responses to normal-weight individuals. Although subjects with diabetes and hypertension had numerically lower antibody titres, this association was not statistically significant. Vitamin D levels showed no clear relationships with antibody titres.Conclusions Age, male gender and tobacco use are negatively associated with antibody titres after COVID-19 vaccination, but our data showed no clear correlation with vitamin D levels.Trial registration number NCT04954651; Results. |
| first_indexed | 2024-04-12T16:00:28Z |
| format | Article |
| id | doaj.art-681a92417ae74e838e5c9d1af05905f1 |
| institution | Directory Open Access Journal |
| issn | 2044-6055 |
| language | English |
| last_indexed | 2025-03-19T22:52:48Z |
| publishDate | 2022-05-01 |
| publisher | BMJ Publishing Group |
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| series | BMJ Open |
| spelling | doaj.art-681a92417ae74e838e5c9d1af05905f12024-10-17T05:10:11ZengBMJ Publishing GroupBMJ Open2044-60552022-05-0112510.1136/bmjopen-2021-057084Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western GreeceMarkos Marangos0Anastasia Parthymou1Evagelia E Habeos2George I Habeos3Apostolos Deligakis4Ektoras Livieratos5Dionysios V Chartoumpekis61 Division of Endocrinology, Department of Internal Medicine, University of Patras, Patras, Greece1 Division of Endocrinology, Department of Internal Medicine, University of Patras, Patras, Greece1 Division of Endocrinology, Department of Internal Medicine, University of Patras, Patras, Greece1 Division of Endocrinology, Department of Internal Medicine, University of Patras, Patras, Greece2 General Hospital of Patras St Andrews, Patras, Greece1 Division of Endocrinology, Department of Internal Medicine, University of Patras, Patras, Greece1 Division of Endocrinology, Department of Internal Medicine, University of Patras, Patras, GreeceObjectives Vaccination against SARS-CoV-2 has been extensively deployed during COVID-19 pandemic. One efficient method to evaluate response to vaccination is the assessment of humoral immunity by measuring SARS-CoV-2 antibody titres. We investigated the association between anthropometric parameters (age, body mass index), smoking, diabetes, statin use, hypertension, levels of 25(OH)D and dehydroepiandrosterone sulfate (DHEAS), and SARS-CoV-2 antibody titres after vaccination.Design In this longitudinal observational cohort study, 712 subjects were tested for SARS-CoV-2 antibodies 3 months after the second dose of BNT162b2 vaccine. Multiple linear regression analysis was performed to identify which factors are associated with the antibody titres.Setting Healthcare units of western Greece (University Hospital of Patras and “St Andrews” State General Hospital of Patras).Participants All adults receiving their second dose of BNT162b2 vaccine at the participating healthcare units were eligible to participate in the study. Exclusion criteria were SARS-CoV-2 infection or positive SARS-CoV-2 antibody titre at baseline. Patients who did not provide all necessary information were excluded from our analyses.Results We found age to be negatively associated with antibody titre (−0.005; 95% CI −0.009 to −0.001, p=0.0073), as was male gender (−0.11; 95% CI −0.1738 to −0.04617, p=0.0008). The interaction of age and gender was significant (−0.01090; 95% CI −0.01631 to −0.005490, p<0.0001), highlighting that the rate of decline in antibody titre with increasing age tends to be higher in men rather than in women. No linear trend was found between DHEAS levels and antibody titres when the lower quartile of DHEAS levels was used as reference. Tobacco use was associated with low antibody titre (−0.1097; 95% CI −0.174 to −0.046, p=0.0008) but overweight, obese or underweight subjects had similar antibody responses to normal-weight individuals. Although subjects with diabetes and hypertension had numerically lower antibody titres, this association was not statistically significant. Vitamin D levels showed no clear relationships with antibody titres.Conclusions Age, male gender and tobacco use are negatively associated with antibody titres after COVID-19 vaccination, but our data showed no clear correlation with vitamin D levels.Trial registration number NCT04954651; Results.https://bmjopen.bmj.com/content/12/5/e057084.full |
| spellingShingle | Markos Marangos Anastasia Parthymou Evagelia E Habeos George I Habeos Apostolos Deligakis Ektoras Livieratos Dionysios V Chartoumpekis Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece BMJ Open |
| title | Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece |
| title_full | Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece |
| title_fullStr | Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece |
| title_full_unstemmed | Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece |
| title_short | Factors associated with anti-SARS-CoV-2 antibody titres 3 months post-vaccination with the second dose of BNT162b2 vaccine: a longitudinal observational cohort study in western Greece |
| title_sort | factors associated with anti sars cov 2 antibody titres 3 months post vaccination with the second dose of bnt162b2 vaccine a longitudinal observational cohort study in western greece |
| url | https://bmjopen.bmj.com/content/12/5/e057084.full |
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