Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage

The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage...

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Main Authors: Sri Wahjuningsih, Desinta Wulandari
Format: Article
Language:English
Published: Fakultas Peternakan Universitas Brawijaya 2019-12-01
Series:Jurnal Ilmu-Ilmu Peternakan
Subjects:
Online Access:https://jiip.ub.ac.id/index.php/jiip/article/view/779
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author Sri Wahjuningsih
Desinta Wulandari
author_facet Sri Wahjuningsih
Desinta Wulandari
author_sort Sri Wahjuningsih
collection DOAJ
description The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage.Â
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spelling doaj.art-681be2346b35442989e124671860569b2023-05-24T03:51:19ZengFakultas Peternakan Universitas BrawijayaJurnal Ilmu-Ilmu Peternakan0852-36812443-07652019-12-0129325926510.21776/ub.jiip.2019.029.03.08579Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storageSri Wahjuningsih0Desinta Wulandari1Faculty of Animal Science, University of Brawijaya, MalangFaculty of Animal Science, University of Brawijaya, MalangThe success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage.Âhttps://jiip.ub.ac.id/index.php/jiip/article/view/779cep-3antioxidantsemen qualitywater clover extract
spellingShingle Sri Wahjuningsih
Desinta Wulandari
Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
Jurnal Ilmu-Ilmu Peternakan
cep-3
antioxidant
semen quality
water clover extract
title Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
title_full Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
title_fullStr Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
title_full_unstemmed Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
title_short Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
title_sort study on the marsilea crenata extract supplementation on caudal epididymal plasma cep 3 to the boer semen quality during cold storage
topic cep-3
antioxidant
semen quality
water clover extract
url https://jiip.ub.ac.id/index.php/jiip/article/view/779
work_keys_str_mv AT sriwahjuningsih studyonthemarsileacrenataextractsupplementationoncaudalepididymalplasmacep3totheboersemenqualityduringcoldstorage
AT desintawulandari studyonthemarsileacrenataextractsupplementationoncaudalepididymalplasmacep3totheboersemenqualityduringcoldstorage