Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage
The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage...
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Format: | Article |
Language: | English |
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Fakultas Peternakan Universitas Brawijaya
2019-12-01
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Series: | Jurnal Ilmu-Ilmu Peternakan |
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Online Access: | https://jiip.ub.ac.id/index.php/jiip/article/view/779 |
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author | Sri Wahjuningsih Desinta Wulandari |
author_facet | Sri Wahjuningsih Desinta Wulandari |
author_sort | Sri Wahjuningsih |
collection | DOAJ |
description | The success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage. |
first_indexed | 2024-03-13T09:54:35Z |
format | Article |
id | doaj.art-681be2346b35442989e124671860569b |
institution | Directory Open Access Journal |
issn | 0852-3681 2443-0765 |
language | English |
last_indexed | 2024-03-13T09:54:35Z |
publishDate | 2019-12-01 |
publisher | Fakultas Peternakan Universitas Brawijaya |
record_format | Article |
series | Jurnal Ilmu-Ilmu Peternakan |
spelling | doaj.art-681be2346b35442989e124671860569b2023-05-24T03:51:19ZengFakultas Peternakan Universitas BrawijayaJurnal Ilmu-Ilmu Peternakan0852-36812443-07652019-12-0129325926510.21776/ub.jiip.2019.029.03.08579Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storageSri Wahjuningsih0Desinta Wulandari1Faculty of Animal Science, University of Brawijaya, MalangFaculty of Animal Science, University of Brawijaya, MalangThe success of artificial insemination (AI) would be determined by the semen quality during storage. This research aims to evaluate the quality of Boer goat liquid semen stored with caudal epididymal plasma (CEP-3) supplemented with water clover (Marsilea crenata) extract or WCE during cold storage (3-5ºC). The semen was collected from Boer goat aged 2.5 to 3 years old by using an artificial vagina. The semen with >2+ mass motility and >70% individual motility were then selected for the study. A randomized block design which consisted of four treatments with ten replications as follow: P0 or control (CEP-3 + 10% egg yolk + 0.4% albumen); P1 (CEP-3 + 10% egg yolk + 0.4% albumen + 1% WCE; P2 (CEP-3 + 10% egg yolk + 0.4% albumen + 2% WCE); P3 (CEP-3 + 10% egg yolk + 0.4% albumen + 3% WCE) was used in this study. The data were analyzed with ANOVA and followed with Duncan’s multiple range test to determine significant differences. The results of this study showed that motility, viability, and membrane integrity in all treatments showed highly significant differences (P<0.01), while no significant difference (P>0.05) was shown on the semen abnormality. The result concludes that 1% WCE supplementation to the CEP-3 extender showed the best Boer semen quality during 96-hours cold storage.Âhttps://jiip.ub.ac.id/index.php/jiip/article/view/779cep-3antioxidantsemen qualitywater clover extract |
spellingShingle | Sri Wahjuningsih Desinta Wulandari Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage Jurnal Ilmu-Ilmu Peternakan cep-3 antioxidant semen quality water clover extract |
title | Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage |
title_full | Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage |
title_fullStr | Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage |
title_full_unstemmed | Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage |
title_short | Study on the Marsilea crenata extract supplementation on caudal epididymal plasma (CEP-3) to the Boer semen quality during cold storage |
title_sort | study on the marsilea crenata extract supplementation on caudal epididymal plasma cep 3 to the boer semen quality during cold storage |
topic | cep-3 antioxidant semen quality water clover extract |
url | https://jiip.ub.ac.id/index.php/jiip/article/view/779 |
work_keys_str_mv | AT sriwahjuningsih studyonthemarsileacrenataextractsupplementationoncaudalepididymalplasmacep3totheboersemenqualityduringcoldstorage AT desintawulandari studyonthemarsileacrenataextractsupplementationoncaudalepididymalplasmacep3totheboersemenqualityduringcoldstorage |