A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach

A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach

The COVID-19 pandemic caused by the SARS-CoV-2 virus, which first emerged in December 2019, represents an ongoing global public health emergency. Here, we developed an improved and highly sensitive approach to SARS-CoV-2 detection via coupling bioluminescence in real-time (BART) and reverse-transcri...

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Bibliographic Details
Main Authors: Zhongjie Fei, Rongbin Wei, Chu Cheng, Pengfeng Xiao
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:International Journal of Molecular Sciences
Subjects:
SARS-CoV-2
accurate and rapid identification
RT-LAMP-BART
digital PCR system
digital RT-LAMP approach
Online Access:https://www.mdpi.com/1422-0067/22/3/1017

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