Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD)
A 32-year old Obsessive-Compulsive Disorder (OCD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochem...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506118302137 |
| _version_ | 1819173674855432192 |
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| author | Ying Wang Chuanyuan Kang Hongxiang Yu Jing Fen Xiquan Ma Ying Lei Jian Zhao |
| author_facet | Ying Wang Chuanyuan Kang Hongxiang Yu Jing Fen Xiquan Ma Ying Lei Jian Zhao |
| author_sort | Ying Wang |
| collection | DOAJ |
| description | A 32-year old Obsessive-Compulsive Disorder (OCD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in OCD.Resource Table.Unlabelled TableUnique stem cell line identifierTUSMi004-AAlternative name(s) of stem cell lineXWInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu@126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell SourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic ModificationNOType of ModificationN/AAssociated diseaseObsessive-Compulsive Disorder(OCD)Gene/locus5-HT1A/chr 5:63258565, rs6295 C-G,LHPP/chr 10:126244970, rs35936514 C-T,HTR1A/chr 63,255,991, rs878567 C-TMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi004-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG)0.016.01.1). |
| first_indexed | 2024-12-22T20:26:50Z |
| format | Article |
| id | doaj.art-681e00cacdb84b97aaad07cc66cc662b |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-22T20:26:50Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-681e00cacdb84b97aaad07cc66cc662b2022-12-21T18:13:43ZengElsevierStem Cell Research1873-50612018-10-01328386Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD)Ying Wang0Chuanyuan Kang1Hongxiang Yu2Jing Fen3Xiquan Ma4Ying Lei5Jian Zhao6Stem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Institutes of Biomedical Sciences & Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University,Shanghai 200032, ChinaDepartment of Psychosomatic Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaStem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaIxCell Biotechnology Co., Ltd, Shanghai, ChinaDepartment of Psychosomatic Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaStem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, ChinaStem Cell Core Facility, Translational Medical Center for Stem Cell Therapy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China; Corresponding author.A 32-year old Obsessive-Compulsive Disorder (OCD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in OCD.Resource Table.Unlabelled TableUnique stem cell line identifierTUSMi004-AAlternative name(s) of stem cell lineXWInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, wangying_csu@126.comType of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell SourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic ModificationNOType of ModificationN/AAssociated diseaseObsessive-Compulsive Disorder(OCD)Gene/locus5-HT1A/chr 5:63258565, rs6295 C-G,LHPP/chr 10:126244970, rs35936514 C-T,HTR1A/chr 63,255,991, rs878567 C-TMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock date08th July,2018Cell line repository/bankhPSCreg, https://hpscreg.eu/user/cellline/edit/TUSMi004-AEthical approvalParticipants informed consent obtained/Ethics Review Board-competent authority approval obtained (EC. D(BG)0.016.01.1).http://www.sciencedirect.com/science/article/pii/S1873506118302137 |
| spellingShingle | Ying Wang Chuanyuan Kang Hongxiang Yu Jing Fen Xiquan Ma Ying Lei Jian Zhao Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD) Stem Cell Research |
| title | Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD) |
| title_full | Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD) |
| title_fullStr | Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD) |
| title_full_unstemmed | Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD) |
| title_short | Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD) |
| title_sort | establishment of tusmi004 a an induced pluripotent stem cell ipsc line from a 32 year old chinese han patient with obsessive compulsive disorder ocd |
| url | http://www.sciencedirect.com/science/article/pii/S1873506118302137 |
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