Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42
Granulosa cells (GCs) are essential for follicular development, and long non-coding RNAs (LncRNAs) are known to support the maintenance of this process and hormone synthesis in mammals. Nevertheless, the regulatory roles of these lncRNAs within sheep follicular GCs remain largely unexplored. This st...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1366759/full |
| _version_ | 1797279259693154304 |
|---|---|
| author | Jinglei Wang Hanying Chen Yongsheng Zhang Hong Shen Xiancun Zeng |
| author_facet | Jinglei Wang Hanying Chen Yongsheng Zhang Hong Shen Xiancun Zeng |
| author_sort | Jinglei Wang |
| collection | DOAJ |
| description | Granulosa cells (GCs) are essential for follicular development, and long non-coding RNAs (LncRNAs) are known to support the maintenance of this process and hormone synthesis in mammals. Nevertheless, the regulatory roles of these lncRNAs within sheep follicular GCs remain largely unexplored. This study delved into the influence of a Loc105611671, on the proliferation and steroid hormone synthesis of sheep ovarian GCs and the associated target genes in vitro. Cell Counting Kit-8 (CCK-8) gain-of-function experiments indicated that overexpression of Loc105611671 significantly boosted GCs proliferation, along with estrogen (E2) and progesterone (P4) levels. Further mechanistic scrutiny revealed that Loc105611671 is primarily localized within the cytoplasm of ovarian granulosa cells and engages in molecular interplay with CDC42. This interaction results in the upregulation of CDC42 protein expression. Moreover, it was discerned that increased CDC42 levels contribute to augmented proliferation of follicular granulosa cells and the secretion of E2 and P4. Experiments involving co-transfection elucidated that the concurrent overexpression of CDC42 and Loc105611671 acted synergistically to potentiate these effects. These findings provide insights into the molecular underpinnings of fecundity in ovine species and may inform future strategies for enhancing reproductive outcomes. |
| first_indexed | 2024-03-07T16:22:39Z |
| format | Article |
| id | doaj.art-6822c33003e74e24ad4cd80e1edeafb1 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-07T16:22:39Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-6822c33003e74e24ad4cd80e1edeafb12024-03-04T04:28:28ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-03-011110.3389/fvets.2024.13667591366759Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42Jinglei Wang0Hanying Chen1Yongsheng Zhang2Hong Shen3Xiancun Zeng4College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, ChinaSchool of Pharmacy, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, ChinaCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, ChinaGranulosa cells (GCs) are essential for follicular development, and long non-coding RNAs (LncRNAs) are known to support the maintenance of this process and hormone synthesis in mammals. Nevertheless, the regulatory roles of these lncRNAs within sheep follicular GCs remain largely unexplored. This study delved into the influence of a Loc105611671, on the proliferation and steroid hormone synthesis of sheep ovarian GCs and the associated target genes in vitro. Cell Counting Kit-8 (CCK-8) gain-of-function experiments indicated that overexpression of Loc105611671 significantly boosted GCs proliferation, along with estrogen (E2) and progesterone (P4) levels. Further mechanistic scrutiny revealed that Loc105611671 is primarily localized within the cytoplasm of ovarian granulosa cells and engages in molecular interplay with CDC42. This interaction results in the upregulation of CDC42 protein expression. Moreover, it was discerned that increased CDC42 levels contribute to augmented proliferation of follicular granulosa cells and the secretion of E2 and P4. Experiments involving co-transfection elucidated that the concurrent overexpression of CDC42 and Loc105611671 acted synergistically to potentiate these effects. These findings provide insights into the molecular underpinnings of fecundity in ovine species and may inform future strategies for enhancing reproductive outcomes.https://www.frontiersin.org/articles/10.3389/fvets.2024.1366759/fullsheepovariesLoc105611671RNA-RNA interactionCDC42 |
| spellingShingle | Jinglei Wang Hanying Chen Yongsheng Zhang Hong Shen Xiancun Zeng Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42 Frontiers in Veterinary Science sheep ovaries Loc105611671 RNA-RNA interaction CDC42 |
| title | Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42 |
| title_full | Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42 |
| title_fullStr | Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42 |
| title_full_unstemmed | Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42 |
| title_short | Long non-coding RNA Loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of CDC42 |
| title_sort | long non coding rna loc105611671 promotes the proliferation of ovarian granulosa cells and steroid hormone production upregulation of cdc42 |
| topic | sheep ovaries Loc105611671 RNA-RNA interaction CDC42 |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1366759/full |
| work_keys_str_mv | AT jingleiwang longnoncodingrnaloc105611671promotestheproliferationofovariangranulosacellsandsteroidhormoneproductionupregulationofcdc42 AT hanyingchen longnoncodingrnaloc105611671promotestheproliferationofovariangranulosacellsandsteroidhormoneproductionupregulationofcdc42 AT yongshengzhang longnoncodingrnaloc105611671promotestheproliferationofovariangranulosacellsandsteroidhormoneproductionupregulationofcdc42 AT hongshen longnoncodingrnaloc105611671promotestheproliferationofovariangranulosacellsandsteroidhormoneproductionupregulationofcdc42 AT xiancunzeng longnoncodingrnaloc105611671promotestheproliferationofovariangranulosacellsandsteroidhormoneproductionupregulationofcdc42 |