Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City
Methicillin-resistant Staphylococcus aureus is an important nosocomial and community pathogen. The genetic determinant of resistance is the mecA gene. 11 types of SCCmec have been described, with types II, III frequently found in hospital infections, and types IV and V in community infections. The p...
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| Format: | Article |
| Language: | English |
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2018-06-01
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| Series: | Kasmera |
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| Online Access: | https://produccioncientificaluz.org/index.php/kasmera/article/view/24652 |
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| author | Sonia Coromoto Romero-Añez Maribel Josefina Castellano-González Armindo José Perozo Mena Gresleida Coromoto Rincón-Villalobos Diana Patricia Zabala-Romero |
| author_facet | Sonia Coromoto Romero-Añez Maribel Josefina Castellano-González Armindo José Perozo Mena Gresleida Coromoto Rincón-Villalobos Diana Patricia Zabala-Romero |
| author_sort | Sonia Coromoto Romero-Añez |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus is an important nosocomial and community pathogen. The genetic determinant of resistance is the mecA gene. 11 types of SCCmec have been described, with types II, III frequently found in hospital infections, and types IV and V in community infections. The present investigation was carried out to study the distribution of the SCCmec types and their relation with the Panton-Valentine Leucocidin, typified by the reaction in the Polymerase Chain. To this end, a total of 42 methicillin-resistant strains carrying the mecA gene were studied. Twenty-nine (29) strains showed the presence of type IV chromosomal cassette (69.05%); 30.95% presented SCCmec type I. A 61.95% (n= 13) of the strains were carriers of SCCmec IV, all of which were positive for the PVL gene. It is worth noting the dissemination of the type IV cassette in intrahospital strains carrying PVL, which is worrisome both for the therapeutic and for the aggravation of infections in patients. |
| first_indexed | 2024-03-12T10:34:36Z |
| format | Article |
| id | doaj.art-6824696f9979497385788108f1f4f5af |
| institution | Directory Open Access Journal |
| issn | 0075-5222 2477-9628 |
| language | English |
| last_indexed | 2024-03-12T10:34:36Z |
| publishDate | 2018-06-01 |
| publisher | Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
| record_format | Article |
| series | Kasmera |
| spelling | doaj.art-6824696f9979497385788108f1f4f5af2023-09-02T08:56:44ZengUniversidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y TropicalesKasmera0075-52222477-96282018-06-01461405124413Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo CitySonia Coromoto Romero-Añez0Maribel Josefina Castellano-González1Armindo José Perozo Mena2Gresleida Coromoto Rincón-Villalobos3Diana Patricia Zabala-Romero4Bacteriología General. Escuela de Bioanálisis. Universidad del Zulia-VenezuelaBacteriología General. Escuela de Bioanálisis. Universidad del Zulia-VenezuelaPráctica Profesional de Bacteriología. Departamento de Microbiología. Escuela de Bioanálisis. Facultad de Medicina. Universidad del Zulia. Centro de Referencia Bacteriológica Hospital Universitario de Maracaibo, Zulia. Venezuela.Bacteriología Clínica. Escuela de Bioanálisis. Universidad del Zulia-VenezuelaEscuela de Medicina. Universidad del Zulia-VenezuelaMethicillin-resistant Staphylococcus aureus is an important nosocomial and community pathogen. The genetic determinant of resistance is the mecA gene. 11 types of SCCmec have been described, with types II, III frequently found in hospital infections, and types IV and V in community infections. The present investigation was carried out to study the distribution of the SCCmec types and their relation with the Panton-Valentine Leucocidin, typified by the reaction in the Polymerase Chain. To this end, a total of 42 methicillin-resistant strains carrying the mecA gene were studied. Twenty-nine (29) strains showed the presence of type IV chromosomal cassette (69.05%); 30.95% presented SCCmec type I. A 61.95% (n= 13) of the strains were carriers of SCCmec IV, all of which were positive for the PVL gene. It is worth noting the dissemination of the type IV cassette in intrahospital strains carrying PVL, which is worrisome both for the therapeutic and for the aggravation of infections in patients.https://produccioncientificaluz.org/index.php/kasmera/article/view/24652Staphylococcus aureus resistente a meticilinatécnicas de tipificación bacterianafactores de virulencia |
| spellingShingle | Sonia Coromoto Romero-Añez Maribel Josefina Castellano-González Armindo José Perozo Mena Gresleida Coromoto Rincón-Villalobos Diana Patricia Zabala-Romero Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City Kasmera Staphylococcus aureus resistente a meticilina técnicas de tipificación bacteriana factores de virulencia |
| title | Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City |
| title_full | Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City |
| title_fullStr | Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City |
| title_full_unstemmed | Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City |
| title_short | Detection of chromosomal cassette in MRSA Staphylococcus aureus strains isolated in a University Hospital in Maracaibo City |
| title_sort | detection of chromosomal cassette in mrsa staphylococcus aureus strains isolated in a university hospital in maracaibo city |
| topic | Staphylococcus aureus resistente a meticilina técnicas de tipificación bacteriana factores de virulencia |
| url | https://produccioncientificaluz.org/index.php/kasmera/article/view/24652 |
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