Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling
Saving space for sperm cryopreservation would aid mouse genetics research. We previously developed the ST (sperm freezing in ShorT STraw to reduce STorage space) method for cryopreserving mouse sperm in a smaller storage space than conventional methods. However, our ST method has two drawbacks: diff...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Society for Reproduction and Development
2021-04-01
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| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/67/3/67_2021-019/_pdf/-char/en |
| _version_ | 1827752941622132736 |
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| author | Ryosuke KANEKO Toshie KAKINUMA Sachiko SATO Atsushi JINNO-OUE |
| author_facet | Ryosuke KANEKO Toshie KAKINUMA Sachiko SATO Atsushi JINNO-OUE |
| author_sort | Ryosuke KANEKO |
| collection | DOAJ |
| description | Saving space for sperm cryopreservation would aid mouse genetics research. We previously developed the ST (sperm freezing in ShorT STraw to reduce STorage space) method for cryopreserving mouse sperm in a smaller storage space than conventional methods. However, our ST method has two drawbacks: difficulties during freeze-thaw procedures and the potential risk of sperm loss during storage. Here, we refine ST, terming the new method improved ST (iST). In iST, the straw has an air-permeable filter and the straw container (2-ml cryotube) is endowed with air vents. As in our ST method, iST frozen-thawed sperm showed good performance upon in vitro fertilization. Moreover, up to nine straws can be stored in one cryotube, occupying less storage space than conventional methods. This method provides an easy and space-saving cryopreservation method for mouse sperm, and thus will be valuable for mouse genetics researchers. |
| first_indexed | 2024-03-11T07:30:42Z |
| format | Article |
| id | doaj.art-6825d7d9cdac4bf688152bb51b233087 |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:30:42Z |
| publishDate | 2021-04-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-6825d7d9cdac4bf688152bb51b2330872023-11-17T07:20:44ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002021-04-0167323523910.1262/jrd.2021-019jrdImprovement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handlingRyosuke KANEKO0Toshie KAKINUMA1Sachiko SATO2Atsushi JINNO-OUE3KOKORO-Biology Group, Laboratories for Integrated Biology, Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, JapanBioresource Center, Gunma University Graduate School of Medicine, Gunma 371-8511, JapanBioresource Center, Gunma University Graduate School of Medicine, Gunma 371-8511, JapanBioresource Center, Gunma University Graduate School of Medicine, Gunma 371-8511, JapanSaving space for sperm cryopreservation would aid mouse genetics research. We previously developed the ST (sperm freezing in ShorT STraw to reduce STorage space) method for cryopreserving mouse sperm in a smaller storage space than conventional methods. However, our ST method has two drawbacks: difficulties during freeze-thaw procedures and the potential risk of sperm loss during storage. Here, we refine ST, terming the new method improved ST (iST). In iST, the straw has an air-permeable filter and the straw container (2-ml cryotube) is endowed with air vents. As in our ST method, iST frozen-thawed sperm showed good performance upon in vitro fertilization. Moreover, up to nine straws can be stored in one cryotube, occupying less storage space than conventional methods. This method provides an easy and space-saving cryopreservation method for mouse sperm, and thus will be valuable for mouse genetics researchers.https://www.jstage.jst.go.jp/article/jrd/67/3/67_2021-019/_pdf/-char/encryopreservationin vitro fertilizationmousespace-savingspermatozoa |
| spellingShingle | Ryosuke KANEKO Toshie KAKINUMA Sachiko SATO Atsushi JINNO-OUE Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling The Journal of Reproduction and Development cryopreservation in vitro fertilization mouse space-saving spermatozoa |
| title | Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling |
| title_full | Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling |
| title_fullStr | Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling |
| title_full_unstemmed | Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling |
| title_short | Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling |
| title_sort | improvement of short straws for sperm cryopreservation installing an air permeable filter facilitates handling |
| topic | cryopreservation in vitro fertilization mouse space-saving spermatozoa |
| url | https://www.jstage.jst.go.jp/article/jrd/67/3/67_2021-019/_pdf/-char/en |
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