Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms
Hazardous impacts caused by harmful algal blooms (HABs) have facilitated the development of novel effective approaches for controlling the frequent HABs. Recently, bacteria with algicidal effects, as a promising alternative tool for eliminating the HABs-forming species have received wide concerns. I...
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| Format: | Article |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1287998/full |
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| author | Sha Wu Sha Wu Jing Tong Jiahuan Chen Minchun Chen Liyan Wang Shuangfei Li Zhangli Hu Huirong Chen |
| author_facet | Sha Wu Sha Wu Jing Tong Jiahuan Chen Minchun Chen Liyan Wang Shuangfei Li Zhangli Hu Huirong Chen |
| author_sort | Sha Wu |
| collection | DOAJ |
| description | Hazardous impacts caused by harmful algal blooms (HABs) have facilitated the development of novel effective approaches for controlling the frequent HABs. Recently, bacteria with algicidal effects, as a promising alternative tool for eliminating the HABs-forming species have received wide concerns. In the present study, a marine bacterial strain 6A1 with potent algicidal activity was successfully isolated from a bloom of Akashiwo sanguinea, and the characteristics related to its algicidal action were systematically investigated. Sequencing results of 16S rDNA suggested that this bacterium belongs to the genus Arenibacter in the family Flavobacteriaceae, while the phylogenetic analysis revealed that Arenibacter sp. strain 6A1 was the only algicidal bacterium in Arenibacter to date, according to the best of our knowledge. Arenibacter sp. strain 6A1 had an extraordinarily high algicidal performance when it was added to A. sanguinea culture with a volume fraction of 1.5% for 2 h. The algicidal performance of strain 6A1 was concentration-dependent and time-dependent. The best algicidal efficacy of strain 6A1 was seen during the stationary phase, while the resistance of A. sanguinea to strain 6A1 declined as the growth cycle progressed. Subsequently, the indirect algicidal mode of Arenibacter sp. strain 6A1 was verified and the algicidal compounds produced by strain 6A1 were proved to be heat-resistant. Further research showed that strain 6A1 caused a severe damage in photosynthesis, oxidative stress, and also damage in three HABs-forming algae to varying degrees. The greatest algicidal performance was found on unarmored dinoflagellate A. sanguinea, followed by raphidophyte Chattonella antiqua and armored dinoflagellate Alexandrium tamarense. The Arenibacter sp. strain 6A1 exhibited selective algicidal activity on a wide range of algal species, primarily targeting Pyrrophyta and Ochrophyta. The proposed bacteria (strain 6A1) can be a powerful and eco-friendly tool for emergency treatment of HABs caused by certain algal species in coastal water bodies. |
| first_indexed | 2024-03-08T09:29:57Z |
| format | Article |
| id | doaj.art-68263652240f44a69fddf974cb810981 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-08T09:29:57Z |
| publishDate | 2024-01-01 |
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| series | Frontiers in Marine Science |
| spelling | doaj.art-68263652240f44a69fddf974cb8109812024-01-31T04:42:56ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452024-01-011010.3389/fmars.2023.12879981287998Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal bloomsSha Wu0Sha Wu1Jing Tong2Jiahuan Chen3Minchun Chen4Liyan Wang5Shuangfei Li6Zhangli Hu7Huirong Chen8Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaCollege of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaGuangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, ChinaHazardous impacts caused by harmful algal blooms (HABs) have facilitated the development of novel effective approaches for controlling the frequent HABs. Recently, bacteria with algicidal effects, as a promising alternative tool for eliminating the HABs-forming species have received wide concerns. In the present study, a marine bacterial strain 6A1 with potent algicidal activity was successfully isolated from a bloom of Akashiwo sanguinea, and the characteristics related to its algicidal action were systematically investigated. Sequencing results of 16S rDNA suggested that this bacterium belongs to the genus Arenibacter in the family Flavobacteriaceae, while the phylogenetic analysis revealed that Arenibacter sp. strain 6A1 was the only algicidal bacterium in Arenibacter to date, according to the best of our knowledge. Arenibacter sp. strain 6A1 had an extraordinarily high algicidal performance when it was added to A. sanguinea culture with a volume fraction of 1.5% for 2 h. The algicidal performance of strain 6A1 was concentration-dependent and time-dependent. The best algicidal efficacy of strain 6A1 was seen during the stationary phase, while the resistance of A. sanguinea to strain 6A1 declined as the growth cycle progressed. Subsequently, the indirect algicidal mode of Arenibacter sp. strain 6A1 was verified and the algicidal compounds produced by strain 6A1 were proved to be heat-resistant. Further research showed that strain 6A1 caused a severe damage in photosynthesis, oxidative stress, and also damage in three HABs-forming algae to varying degrees. The greatest algicidal performance was found on unarmored dinoflagellate A. sanguinea, followed by raphidophyte Chattonella antiqua and armored dinoflagellate Alexandrium tamarense. The Arenibacter sp. strain 6A1 exhibited selective algicidal activity on a wide range of algal species, primarily targeting Pyrrophyta and Ochrophyta. The proposed bacteria (strain 6A1) can be a powerful and eco-friendly tool for emergency treatment of HABs caused by certain algal species in coastal water bodies.https://www.frontiersin.org/articles/10.3389/fmars.2023.1287998/fullArenibacter sp. strain 6A1Akashiwo sanguineaalgicidal bacteriaalgicidal activityharmful algal blooms (HABs) |
| spellingShingle | Sha Wu Sha Wu Jing Tong Jiahuan Chen Minchun Chen Liyan Wang Shuangfei Li Zhangli Hu Huirong Chen Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms Frontiers in Marine Science Arenibacter sp. strain 6A1 Akashiwo sanguinea algicidal bacteria algicidal activity harmful algal blooms (HABs) |
| title | Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms |
| title_full | Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms |
| title_fullStr | Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms |
| title_full_unstemmed | Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms |
| title_short | Characterization of a novel algicidal bacteria Arenibacter sp. strain 6A1 and its application to eliminate harmful algal blooms |
| title_sort | characterization of a novel algicidal bacteria arenibacter sp strain 6a1 and its application to eliminate harmful algal blooms |
| topic | Arenibacter sp. strain 6A1 Akashiwo sanguinea algicidal bacteria algicidal activity harmful algal blooms (HABs) |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1287998/full |
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