Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration
Abstract Peripheral nerve regeneration after injury is still a clinical problem. The application of autologous nerve grafting, the gold standard treatment, is greatly restricted. Acellular nerve allografts (ANAs) are considered promising alternatives, but they are difficult to achieve satisfactory t...
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| Format: | Article |
| Language: | English |
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Wiley
2023-07-01
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| Series: | Bioengineering & Translational Medicine |
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| Online Access: | https://doi.org/10.1002/btm2.10435 |
| _version_ | 1797777270368108544 |
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| author | Tianhao Yu Qiang Ao Tianrang Ao Muhammad Arslan Ahmad Aijun Wang Yingxi Xu Zhongti Zhang Qing Zhou |
| author_facet | Tianhao Yu Qiang Ao Tianrang Ao Muhammad Arslan Ahmad Aijun Wang Yingxi Xu Zhongti Zhang Qing Zhou |
| author_sort | Tianhao Yu |
| collection | DOAJ |
| description | Abstract Peripheral nerve regeneration after injury is still a clinical problem. The application of autologous nerve grafting, the gold standard treatment, is greatly restricted. Acellular nerve allografts (ANAs) are considered promising alternatives, but they are difficult to achieve satisfactory therapeutic outcomes, which may be attributed to their compact inherent ultrastructure and substantial loss of extracellular matrix (ECM) components. Regarding these deficiencies, this study developed an optimized multichannel ANA by a modified decellularization method. These innovative ANAs were demonstrated to retain more ECM bioactive molecules and regenerative factors, with effective elimination of cellular antigens. The presence of microchannels with larger pore size allowed ANAs to gain higher porosity and better swelling performance, which improves their internal ultrastructure. Their mechanical properties were more similar to those of native nerves. Moreover, the optimized ANAs exhibited good biocompatibility and possessed significant advantages in supporting the proliferation and migration of Schwann cells in vitro. The in vivo results further confirmed their superior capacity to promote axon regrowth and myelination as well as restore innervation of target muscles, leading to better functional recovery than the conventional ANAs. Overall, this study demonstrates that the optimized multichannel ANAs have great potential for clinical application and offer new insight into the further improvement of ANAs. |
| first_indexed | 2024-03-12T23:01:46Z |
| format | Article |
| id | doaj.art-68272fba987f47a1968f629b575b7389 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-03-12T23:01:46Z |
| publishDate | 2023-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-68272fba987f47a1968f629b575b73892023-07-19T09:59:03ZengWileyBioengineering & Translational Medicine2380-67612023-07-0184n/an/a10.1002/btm2.10435Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regenerationTianhao Yu0Qiang Ao1Tianrang Ao2Muhammad Arslan Ahmad3Aijun Wang4Yingxi Xu5Zhongti Zhang6Qing Zhou7The VIP Department, School and Hospital of Stomatology China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases Shenyang ChinaDepartment of Developmental Cell Biology, Key Laboratory of Medical Cell Biology, Ministry of Education China Medical University Shenyang ChinaCancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaCollege of Life Sciences and Oceanography Shenzhen University Shenzhen ChinaDepartment of Neurological Surgery University of California Davis Sacramento California USADepartment of Clinical Nutrition Shengjing Hospital of China Medical University Shenyang ChinaThe VIP Department, School and Hospital of Stomatology China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases Shenyang ChinaDepartment of Oral and Maxillofacial Surgery, School and Hospital of Stomatology China Medical University, Liaoning Provincial Key Laboratory of Oral Diseases Shenyang ChinaAbstract Peripheral nerve regeneration after injury is still a clinical problem. The application of autologous nerve grafting, the gold standard treatment, is greatly restricted. Acellular nerve allografts (ANAs) are considered promising alternatives, but they are difficult to achieve satisfactory therapeutic outcomes, which may be attributed to their compact inherent ultrastructure and substantial loss of extracellular matrix (ECM) components. Regarding these deficiencies, this study developed an optimized multichannel ANA by a modified decellularization method. These innovative ANAs were demonstrated to retain more ECM bioactive molecules and regenerative factors, with effective elimination of cellular antigens. The presence of microchannels with larger pore size allowed ANAs to gain higher porosity and better swelling performance, which improves their internal ultrastructure. Their mechanical properties were more similar to those of native nerves. Moreover, the optimized ANAs exhibited good biocompatibility and possessed significant advantages in supporting the proliferation and migration of Schwann cells in vitro. The in vivo results further confirmed their superior capacity to promote axon regrowth and myelination as well as restore innervation of target muscles, leading to better functional recovery than the conventional ANAs. Overall, this study demonstrates that the optimized multichannel ANAs have great potential for clinical application and offer new insight into the further improvement of ANAs.https://doi.org/10.1002/btm2.10435acellular nerve allograftdecellularizationextracellular matrixmultichannelnerve regenerationperipheral nerve injury |
| spellingShingle | Tianhao Yu Qiang Ao Tianrang Ao Muhammad Arslan Ahmad Aijun Wang Yingxi Xu Zhongti Zhang Qing Zhou Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration Bioengineering & Translational Medicine acellular nerve allograft decellularization extracellular matrix multichannel nerve regeneration peripheral nerve injury |
| title | Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration |
| title_full | Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration |
| title_fullStr | Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration |
| title_full_unstemmed | Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration |
| title_short | Preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration |
| title_sort | preparation and assessment of an optimized multichannel acellular nerve allograft for peripheral nerve regeneration |
| topic | acellular nerve allograft decellularization extracellular matrix multichannel nerve regeneration peripheral nerve injury |
| url | https://doi.org/10.1002/btm2.10435 |
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